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EMAGE:7040

Mrpl51 mitochondrial ribosomal protein L51 ( MGI:1913743)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7040 EMAGE:7040 EMAGE:7040 EMAGE:7040 EMAGE:7040
"Pseudo-wholemount" of euxassay_002497. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002497_01 euxassay_002497_02 euxassay_002497_03 euxassay_002497_04
EMAGE:7040 EMAGE:7040 EMAGE:7040 EMAGE:7040 EMAGE:7040
euxassay_002497_05 euxassay_002497_06 euxassay_002497_07 euxassay_002497_08 euxassay_002497_09
EMAGE:7040 EMAGE:7040 EMAGE:7040 EMAGE:7040 EMAGE:7040
euxassay_002497_10 euxassay_002497_11 euxassay_002497_12 euxassay_002497_13 euxassay_002497_14
EMAGE:7040 EMAGE:7040 EMAGE:7040 EMAGE:7040 EMAGE:7040
euxassay_002497_15 euxassay_002497_16 euxassay_002497_17 euxassay_002497_18 euxassay_002497_19
EMAGE:7040 EMAGE:7040 EMAGE:7040 EMAGE:7040
euxassay_002497_20 euxassay_002497_21 euxassay_002497_22 euxassay_002497_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7040Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7040_wholemount_strong.wlz
7040_wholemount_moderate.wlz
7040_wholemount_weak.wlz
7040_wholemount_possible.wlz
7040_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7040_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5149
Entity Detected:Mrpl51, mitochondrial ribosomal protein L51 ( MGI:1913743)
Sequence:sense strand is shown

>T5149
CCACGCGTCCGGGGTAAGGCCCAAAATGGCTCCTCTTACTGTCACTACCGTGTGAGTCCCGCCTGGGACT
GTAATCTGTGTCTTGGCTTTGGAATTCACACCCGCTCCCCATTTTTCCGGCTGGTTTGCTGCCCCTACTG
AGGCGGACAGGCTAGAGTAATGGCAGGGAGTGTCCCTTGGGCGGCAAGTAGGAGACTGTGGGGCTGGGTG
CCCTCGGCATGCCGAAGCTTCTCTCTGGGCGTCCCACGGTTGGCCTTCGTAAGGCTCACTCTTCCACCTC
CCAAAGTGGTTGATCGCTGGAACGAGAAGAGGGCTCTGTTTGGCGTCTACGACAACATTGGAATCCTGGG
GAATTTCGAGAAGCACCCAAAAGAACTGATCAAGGGCCCAGTATGGCTTCGAGGCTGGAGAGGAAATGAA
TTGCAGCGTTGTGTCCGAAAGAAGAAATTTGTGGGAAACAGGATGTTCATTGAGGACCTGCATAACCTTA
ACAAACGGATCAGCTATCTCTACAAACACTTTAACCGACATGGGAAGTACAGATAGGACGGGAAATGTGT
CACCCTGGAGAAGGGGAACAGGACTTATCCCAGCAAGGAGAGAGCTTTGTGTGCTCGATGTAATAAAAAA
ATGGGGCTCCTTTGGAATCTGATATGCCCAGTGCTACATTTACTCAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5344672 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5344672 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7039 same embryo
 EMAGE:7041 same embryo
 EMAGE:7038 same embryo
 EMAGE:7037 same embryo
 EMAGE:7036 same embryo
 EurExpress:euxassay_002497 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS