EMAGE mouse gene expression spatial database

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EMAGE:7074

E2f6 E2F transcription factor 6 ( MGI:1354159)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002536. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002536_01	euxassay_002536_02	euxassay_002536_03	euxassay_002536_04

euxassay_002536_05	euxassay_002536_06	euxassay_002536_07	euxassay_002536_08	euxassay_002536_09

euxassay_002536_10	euxassay_002536_11	euxassay_002536_12	euxassay_002536_13	euxassay_002536_14

euxassay_002536_15	euxassay_002536_16	euxassay_002536_17	euxassay_002536_18	euxassay_002536_19

euxassay_002536_20	euxassay_002536_21	euxassay_002536_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7074_wholemount_strong.wlz

7074_wholemount_moderate.wlz

7074_wholemount_weak.wlz

7074_wholemount_possible.wlz

7074_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7074_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5037
Entity Detected:	E2f6, E2F transcription factor 6 ( MGI:1354159)
Sequence:	sense strand is shown >T5037 GGCCGGCCAGGGAACCTGAGCGCCTCCGCGGTCACCCTGGTCCCCTCCGAGCCTACCCTGACCCCCTCTG CGCCTGGGCTCATCGCTGCGCGAGTGGGACCGCGGCGCTTCGTGGGTGGAGCCGCAGTGCTCCGTGGGTG GAGCGGGCGGCGCTGAAGGGGCCGGGAACGCGCGCGCCGAGCCTCCGGGGAAGGCGGGAGGGCGCGTGGG GCGCGAGGGGCGCGGGACAGCATGAGTCAGCAACGGACGGCGCGGAGACTGCCCAGCCTGCTGGTGGACC CGGCGCAGGAAACGGTGCGCCGGCGTTGCCGGGACCCCATCAACGTGGAAAACCTACTGCCATCAAAAAT AAGGATTAATCTAGAAGAAAATGTACAGTATGTGTCCATGAGAAAAGCTCTGAAAGTGAAGAGGCCCCGG TTTGATGTGTCACTGGTATACTTAACTCGGAAGTTTATGGATCTCGTCAGATCTGCCCCTGGGGGCATTC TTGACTTAAACAAAGTTGCCACAAAACTGGGTGTTCGGAAGAGGCGAGTGTATGACATCACCAATGTCTT GGATGGCATCGAACTGGTGGAAAAGAAATCTAAGAACCACATTCGGTGGATAGGATCTGACCTGAACAAC TTTGGGGCCGCACCCCAGCAGAAGAAGCTGCAGGCAGAGCTCTCCGACCTGTCGGCCATGGAAGACGCCT TGGACGAGTTGATTAAAGATTGTGCTCAGCAACTGTTGGAGTTAACAGATGACAAGGAAAATGAAAGACT AGCGTATGTAACCTATCAGGATATTCACGGCATTCAAGCTTTCCATGAACAGATTGTCATTGCAGTGAAG GCTCCAGAGGAAACCAGACTGGATGTTCCAGCTCCCAGAGAAGATTCTATCACAGTACATATTAGGAGCA CCAAAGGACCCATTGATGTATATTTGTGTGAAGTAGAACAGAACCATTCAAATGGTAAAACCAATGATGG AATAGGAGCCTCTCCATCTAAAAGCAGCCATCCACAATGCCCAGAGAAAGAAGACGAGCCTCCTCAGTGA GTGAAGAACTCAGAGTGAGCGGCTGGTAGCAGTGCAGAGTGACGAAGGTTTTTGGAGTATCCCGAGTGGG TGAATCATGTGCCCCATCCAGTGTGGGTGAATCATGTGCCCATCGTTTGATTTTCAGCAATAAGTCTCCT CGGAGTGCTCTTGTGCTCTATTGTAGACTGGTAGCCGCACCGAAGGCCAGTAGTGTTCTCCCGAGTCGCC TCCAGCACGGGGTCCTGATGGGTTCCCCATCCAGAATCAGCTCTCGAGATAACTCTTCGCTGCCCTTGGT CAGTGAAATAGACCTCTTGCCTCCTGAAATCATGTTATCACAAAGACTAGCATAAGGACAGACAGCTGGA CAGCTGGTCGTGGACTGTGCCCTGCCCTGATGGATAGTGTTTGCCTCACAGAAGAATTGCCTTACACTGG TTCACATTTGCAGTTAGGTGTTGTGCACTGGTGAGGTGCACACACGGGGCCAAATGTGGCACCTTTGTAT ATATCTGTATCATGTTTAGAGGACTTTCAAAATATGTCTGAGTGTCACTTATACCCTAGAACACCCAAAA TAATGTATGTAGGGAAAATGACAGACAGATCTCTAGTTTCTCAGTTCCTATAACTTATTAGAACCCTCTG GATGGGGATGAGAGAGTTTTCTAAAGTTTTACATGTAGAAGTTTCACAGATGCCTGGACATTTAGGTTCA TATACTTAAAGTATTTTAATACGCTCCCCAGTGCTACAATTGGAACCACAAACTTCTTGATTTTTAAGCT GCCTACAGAGTCATCATGTCCCAGAAGATTGGTGGCATGCCTCAAAGCATTGTGGGAAAGTACAGAATTT CATCTGGCAACAGATCAGTTCACTACAATGTGATTTGTTCCTGGTGAAGTTAAAAGCATTATTCCGCTTT TTGTGAGCAGATTTTATAATGTGTTTCTGTGTGCAGCCTTGCTCTTCAGTCAGAGGGCCTTACCTCCTGT GCATTGTACCCCGCCTCAGCTCTCAGCCCGCCTTCCCAATCACCTAGCGCTCTCTACCTCAGTGGGTAAA TCACTTACCTCTCTGTGCCTCAATTGCCTCAGTCATTTACCACTAGACTGTGAGCTCCGTGAGAGACAAT TGTTCATAGTGGCTCCCAGCGCCTTGTACCACGCCTAGTCCAAGACAAGTGCCCAGTGAATGTCTAGCAA ACTCGTGAGTGAACGAGTGCAATGTGGGGCTGATGGAACCTGGATTGTTGGTATACAGGAGTCTGGGAAG CATCGTTAGTTTGGTAAACCTCTCCTGCTTTACAAAAGATTTGAAGCAGCTTGAAATAACAATCATGTAT ACAAAGTAGAGATTCAAAATTAAAGTATTAAAAAAATAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5289475 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5289475 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7072 same embryo
	EMAGE:7077 same embryo
	EMAGE:7076 same embryo
	EMAGE:7075 same embryo
	EMAGE:7073 same embryo
	EurExpress:euxassay_002536 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source