EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7075

Slc44a3 solute carrier family 44, member 3 ( MGI:2384860)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002530. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002530_01	euxassay_002530_02	euxassay_002530_03	euxassay_002530_04

euxassay_002530_05	euxassay_002530_06	euxassay_002530_07	euxassay_002530_08	euxassay_002530_09

euxassay_002530_10	euxassay_002530_11	euxassay_002530_12	euxassay_002530_13	euxassay_002530_14

euxassay_002530_15	euxassay_002530_16	euxassay_002530_17	euxassay_002530_18	euxassay_002530_19

euxassay_002530_20	euxassay_002530_21	euxassay_002530_22	euxassay_002530_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7075_wholemount_strong.wlz

7075_wholemount_moderate.wlz

7075_wholemount_weak.wlz

7075_wholemount_possible.wlz

7075_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7075_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

moderate

regional

moderate expression: see section 11 12 13

pancreas

strong

regional

strong expression: see section 07 08 11 12 13 moderate expression: see section 09 14

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 05 06 07 08 13 14 15

stomach

strong

regional

strong expression: see section 03 04 05 06 07 moderate expression: see section 08

hindgut

moderate

regional

moderate expression: see section 11 12 13

midgut

moderate

regional

moderate expression: see section 11 12 13 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5058
Entity Detected:	Slc44a3, solute carrier family 44, member 3 ( MGI:2384860)
Sequence:	sense strand is shown >T5058 CCACGCGTCCGTAGGGGACCAGCGCCCTGGACCTCTAGGAGGCGCTGAGCCCTGCTCGCCCTGCCGCCCC TCCTGGGCCACTTGGCCCGGTCGCCGCACCTCTGTCCCGTGTGCAAGGCGACCACCGCCGCCGCCAATCC CAGATGCCGGGTACCATGCAGTGCTTGGGCGCCGAGTACCTGGTTTCTGCAGAGAAAACAAGGCAAAGAG AATGGCGACCCCAAATTTATAGAAAATGCACCGATACACCGTGGCTAGTCTTGTTCTTTCTCTTTTGGAC TGGTTTGGTGTTCATCATGGGTTATTCGGTGGTGGCCGGGGCAGCAGGAAGACTCCTCTTTGGATATGAC AGCTTTGGCAACGTGTGTGGCAAGAAGAACTCCCCAGTGGAAGGAGCCCCCCTCTCCGGGCAGGACATGA CTCTAAAAAAACACGTGTTCTTTATGAATGCCTGCAATCTTGAAGTCAAAGACAGGGGTCTTGGTCCCAC AGCCCTCTGTGTATCCAGCTGTCCTGAGAAGCAGCTGGACACTCTGGAAGAGGTCCAGCTTTTTGCCAAC ATCAACGGCTCTTTCCTGTGTGTGTATAGTTTGAATTCCTTCAACTACACTCAGAGTTCAAGTGCAGACA CACTGTGTCCCAGGCTCCCGGTTCCTCCAAGCAAGCCCTTTCCCTTATTTAACCGGTGCATCCCTCAGAC ACCCGAGTGCTATTCCTTGTTTGCATCAGTCTTGATAAATGATGCGGACGCTCTCCATCGGATTCTAAGT GGAATAATGGCGGGAAGAGACACCATCCTCGGCCTGTGTGTCTTCACATTCGCCCTGTCTCTGGCCATGT TGTTCGCCTTCCGGTTCATCTCGACCCTTCTGATCCACATTATCATCTCCTTGGTCATTTTGGGATTGCT GTTCGTCTGCGGTGTCTTATGGTGGCTGTATTATGACTACACCAACGACCTCAGTACAGAACTGGACACA GAAAAGGAGAATATGAATTGTATGCTAGCCTTTGCCATCATCTCTACAGTCGTGACAGTCCTTCTCCTGG CCTTGATTTTTACCCTCAGGAAGAGGATAAAATTGACAGTTGAGCTTCTCCACGTCACAAACAAAGCCAT CAGCAGCTGTCCTTTCCTGCTGTTCCAGCCACTGTGGACGTGTGCCATCCTCATTTTCTTCTGGGTCCTC TGGGTGGCTGTGCTGCTGAGCCTGGGCACTGCTGGTACTGCCCAGGTGATGGAAGGTGGCCAAGTGGAAT ATAAGCCTCTTTCCGGCATTCGGTATATGTGGTGGTACCATTTAATTGGCCTCATCTGGACTAGCGAATT CATCCTTGCATGCCAGCGGATGACTGTAGCAGGAGCAATGGTTGCTTGTTATTTCAACAGGAATCAAAAT GACCCTCCTGCCCGTCCAATCCTTTCGTCTCTCTTCGTACTTTTCTGCTACCATCAAGGAACTGCGGTGA AAGGGTCATTTTTACTCACTGTGACAAGGATCCCAAGAGTCATTTTCATGTGTATTTATAGCACTCTGAA AGAGCCGCAGCGCAGTGCTTGGTCAAGAGGTGAATTCAGATGCAGCCACTGTGGGCTTTGGTGTCTCCTT AAATATCTGTACCATCTCAACCAGGATGCGTACACTGCAACTGCCATTAACGGGACAGACTTTTGTACAT CCGCAAAAGATGCGCACACAATCATAGCCAAGAATTCAAGTCATCTCACGTCTGTTAACTGCTTTGGAAA CTTTGTCATTTTCCTGGGAAAGGTGCTAGTGGTGTGCTTCAGCATTTTCGGGGGTCTGATGGCGTTTAAC TACAGCCGTGCGCTCCAGGTGTGGGCCATCCCCCTGCTGCTGGTTGCCTTTTTTGCCTGTGTGGTGGCTC ACAGTTTCCTATCCGTGTTTGAAACTGCGCTGGATATCCTTTTCCTGTGCTTTGCTGTGGACCTGGAAAC AAATGACGGATCGTCGGAGAAACCGTACTTTATGGACCCAGGGTTTTTGAGTGTTATAAAACGGACCAAC AACTTGAACAATGCCAAGTCTCAAGGACAAAAGGATGCTTTACCAAATGAAGAGGGGACAGAACTCCAGC CCATTGTGAGATAGGGACCAAGAGAACTTTGACTACCTGTGACACCGAGACAAAGTCTACAGCTGTTTTC ACGTGAACTAGAGCACGGAAGATGTTAAAACTATGTCTATATGCATCAATTTAAAAGGCACAATATTGGC TGGGCACAGGGACACACATCTGTAATTCAAAACTGGAAGAGGCTATAAGGCTAGCCTGAGCTACATACTC AGATGTTAAATGCTAGCCTGAATGCTACATAGAGACCTCATATAAACAAACAAACAAATAAGGTTGGCTC ACTGGATAAAGGTGCCAGTTGCCAACCCGAGGAACTGAATACCCCAGGACCCACATGGTAAAAGGAGAGA ATCAGTTCCTCTAAGTTGTCCTCAGAGCTCCACATGTGAACTGCCCCGAGTGCACTCACTCAGCCCCACC ACCACCACCAAAAAATAAATAAAAATGTTAACACCTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5320841 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5320841 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7072 same embryo
	EMAGE:7077 same embryo
	EMAGE:7076 same embryo
	EMAGE:7074 same embryo
	EMAGE:7073 same embryo
	EurExpress:euxassay_002530 same experiment
	MGI:4828234 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source