EMAGE mouse gene expression spatial database

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EMAGE:7076

C87436 expressed sequence C87436 ( MGI:2141787)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002484. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002484_01	euxassay_002484_02	euxassay_002484_03	euxassay_002484_04

euxassay_002484_05	euxassay_002484_06	euxassay_002484_07	euxassay_002484_08	euxassay_002484_09

euxassay_002484_10	euxassay_002484_11	euxassay_002484_12	euxassay_002484_13	euxassay_002484_14

euxassay_002484_15	euxassay_002484_16	euxassay_002484_17	euxassay_002484_18	euxassay_002484_19

euxassay_002484_20	euxassay_002484_21	euxassay_002484_22	euxassay_002484_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7076_wholemount_strong.wlz

7076_wholemount_moderate.wlz

7076_wholemount_weak.wlz

7076_wholemount_possible.wlz

7076_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7076_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5070
Entity Detected:	C87436, expressed sequence C87436 ( MGI:2141787)
Sequence:	sense strand is shown >T5070 CCACGCGTCCGCGCGGCGAGGGTCGTCACCAACGAGATTCCTAGAGTTGCGAGGCAGGCTGCCAAGAGCT CTACCTAGAGGAGTTGGAATCTGGAACCCTGAGCAAGAGCTTTGCTAGAAGGGGATGGAATGTCTGCAAT GTTACATCAGAACTCAGCCTTCAGAGGACTTAGGCCAAGTGGCACACCTCACTGCAAATGAGCATGCCTG GGTTTGTGCTGTCTGATCATCACAAGCTAATGAACCGCGTTAGGTCTCACAGACACAGCTTCCACTGTTC TCCGGGTGCCTTGGCAGCTGGTACAGGGGCTGTGAACAATGGAACCAAACTCTCAGAGGACTAAAGTCCC AGCTTTCTTATCTGACTTGGGGAAGGCCACACTGAGGGGAATCAGGAAGTGTCCCCGATGTGGTACGTTC AATGGAACCCGTGGGTTGAGCTGTAAGAACAAGACATGTGGAACCATATTTCGCTATGGTGCCCGAAAGC AGCCTAGCATTGAAGCTGTCAAAATCATCACCGGCTCTGATCTACAGGTCTACTCTGTGCGACAGAGAGA CCGAGGCCCCGACTACCGATGCTTCGTGGAGCTCGGTGTTTCGGAGACTGCAATCCAGACCGTGGATGGT ACAATCATCACTCAGCTGAGCTCCGGCCGATGTTACGTCCCCTCGTGTTTGAAAGCTGCCACTCAAGGCA TTGTGGAAAACCAGTGTCAACACATCAAGCTAGCGGTGACCTGCCAGGCGGAGGCCACCCCTCTGACCCT GAAGAGCTCAGTCCTGAATGCTCTGCAGGCTGCCCCAGAAACCAAGCAGAGCCTCTGGCAGCTGGCCACC GAGCCCACGGGGCCCCTGGTACAGAGAGTCACTAAAAACATTATGGTGGTGAAATGCAAAGCCAGCCAGA AGCACAACTTGGGCTACTTGCACGCGTCCTTCATGCAGAAGATCAGCTCCAGAAGCTTGCCGGAGCGCCG CTTCTTCTGCTCCTGCCAGACTCTGAGGCCACACAAGTCCAGCGTCCCCAAGGCTGAGGCAGCCCCAAAG TGCATCCATTTCTTTGCTTGTCTCTGTGCCTTTGCCAGCGATGAGACATTGGCTCAGGAGTTCTCGGACT TTCTGAACTTCGATGCCAGTGGTCTTAAGGAGATAATTGTGCCCCATTTAGGCTGCCATGCAGAGTCATC TGTCTCAGCTTGTGAGTCTGCTGCCTCTAAGCCCCGGAAGAGGAAGAAGGATGAAGTGTCTGGTGCACAG GTGAACAGCTCACTGATGCCGCAAGATGCAGTAAACAGCAATCTAAGGAAGAGTGGCCTGAAAAGGCCTG TGGTTACGTCATCATTGAAAAGGCACGTCTGTGGTCAGCTGTTAGATGAGGCACAAGTGACCCTGTCCTT CCAAGACTGGCTGGCCAGTGTCACAGAGCGCATCCATCAAACCATGCACTATCAGTTTGATGGCAAACCA GAACCCCTGGTGTTCCACATTCCTCAGTCCTTTTTTGATGCCTTGCAACAGAGAATATCCATAGGAAGTG CAAAGAAGCGGCTCCCCAACTCCACTACAGCTTTTGTTCGGAAAGATGCCTTGCCACTGGGAACCTTTTC CAAGTACACCTGGCATATCACCAATATTCTGCAAGTCAAACAAATCTTAGACACCCCAGAGATGCCCCTG GAAATCACACGTAGCTTTATCCAGAACCGGGATGGAACTTACGAGCTGTTTAAATGCCCCAAAGTGGAAG TAGAGAACATAGCGGAAAGCTACGGCCGCATAGAGAAGCAGCCTGTGCTGAGACCTTTGGAGCTGAAAAC CTTCCTCAAAGTTGGTAACACTTCCCCAGATCAAAAGGAGCCAACGCCCTTTATCATTGAGTGGATCCCA GATATCCTCCCACAGTCCAAGATTGGTGAGCTTCGAATCAAGTTTGAGTATGGTCACCACCGGAATGGAC ATGTGGCTGACTACCAGGACCCAAGGCCACCCATGGACCAACCTTTGGAGCTGGCCCCTCTGACTACTAT CACTTTCCCTTGAGGCAAATCAAGAGTATTTTGCAGAGTTTTGCACATCCCAATCCCTGAGGACTATAAA TCCTAGTAGCACTTATACGATCCCTGCTAACGAACCACACTTGGCTAAGATGCAGTTACTCAGCCCATCC ATGTGTGTTCTGTAGAAGAAACTCTTGTAAATAGCCTTGTGTGTACAGTCTTCATACAAGTCGGGCACTT ATTTCTGACCAGAGTTTTAGAAGAAACAACAAAAATCCAGCTCACTGGCTTCTCATGGATTCACCTGCAG CTTGTTTTTCTGTAATTCTCCAAAAGTCACAGCACTGGATCTTAGCCAACAGTGTTGGAAAGTTAACGAT GCCCTGGGCCGAGCAGGGTCCTCTCTCTCTGCCCAAGAGCTCCAATCACTCTTTATTCTGATAACAAAAT AAAGTACAGTGTCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5321669 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5321669 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7072 same embryo
	EMAGE:7077 same embryo
	EMAGE:7075 same embryo
	EMAGE:7074 same embryo
	EMAGE:7073 same embryo
	EurExpress:euxassay_002484 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source