EMAGE mouse gene expression spatial database

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EMAGE:7105

Mbd4 methyl-CpG binding domain protein 4 ( MGI:1333850)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002606. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002606_01	euxassay_002606_02	euxassay_002606_03	euxassay_002606_04

euxassay_002606_05	euxassay_002606_06	euxassay_002606_07	euxassay_002606_08	euxassay_002606_09

euxassay_002606_10	euxassay_002606_11	euxassay_002606_12	euxassay_002606_13	euxassay_002606_14

euxassay_002606_15	euxassay_002606_16	euxassay_002606_17	euxassay_002606_18	euxassay_002606_19

euxassay_002606_20	euxassay_002606_21	euxassay_002606_22	euxassay_002606_23	euxassay_002606_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7105_wholemount_strong.wlz

7105_wholemount_moderate.wlz

7105_wholemount_weak.wlz

7105_wholemount_possible.wlz

7105_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7105_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5222
Entity Detected:	Mbd4, methyl-CpG binding domain protein 4 ( MGI:1333850)
Sequence:	sense strand is shown >T5222 GTTGATTGACTTTCACGTCGGTGTTTGGATTCGGCTCAGTGGTTTGAGTTGTGTTTTACTCCACCTCTCG GCCTGGAGCTGCAGCTGCGCCCCAGTGGACCAAAGCAGCAGGGATGGAGAGCCCAAACCTTGGGGACAAC AGAGTCCGTGGAGAGAGCCTAGTTCCAGACCCGCCTTGGGATCGCTGCAAGGAAGATATTGCTGTTGGAC TGGGAGGAGTGGGAGAAGATGGAAAGGACCTGGTGATAAGCAGTGAGCGCAGCTCCCTTCTCCAAGAGCC CACTGCTTCTACTCTGTCTAGTACTACAGCGACAGAAGGCCACAAGCCTGTCCCGTGTGGATGGGAAAGA GTTGTGAAGCAAAGGTTATCTGGGAAAACTGCAGGAAAATTTGATGTATACTTTATCAGCCCACAAGGAT TGAAGTTCAGATCAAAACGTTCACTTGCTAATTATCTTCTCAAAAATGGGGAGACTTTTCTTAAGCCTGA AGATTTTGATTTTACTGTACTGCCGAAAGGGAGCATCAATCCCGGTTATAAACACCAAAGTTTGGCAGCT CTGACTTCCCTGCAGCCAAATGAAACTGACGTTTCAAAGCAGAACCTCAAGACACGAAGCAAGTGGAAAA CAGATGTGTTGCCTCTGCCCAGTGGTACTTCAGAGTCGCCAGAAAGCAGCGGACTGTCTAACTCTAACTC GGCTTGCTTGCTATTGAGAGAACATAGGGACATTCAGGATGTTGACTCTGAGAAGAGGAGAAAGTCCAAA AGAAAGGTGACTGTTTTGAAAGGAACTGCAAGTCAGAAAACCAAACAAAAGTGCAGGAAGAGTCTCTTAG AGTCTACTCAAAGAAACAGAAAAAGAGCATCTGTGGTTCAGAAGGTGGGTGCTGATCGCGAGCTGGTGCC ACAGGAAAGTCAACTCAACAGAACCCTCTGCCCTGCAGATGCCTGTGCAAGGGAGACTGTTGGCCTGGCT GGGGAAGAAAAATCACCAAGCCCAGGACTGGATCTTTGTTTCATACAAGTAACTTCTGGCACCACAAACA AATTCCATTCAACTGAAGCAGCAGGTGAAGCAAATCGTGAGCAGACTTTTTTAGAATCAGAGGAAATCAG ATCGAAGGGAGACAGAAAGGGGGAGGCACATTTGCATACCGGTGTTTTACAGGATGGCTCTGAAATGCCC AGCTGCTCACAAGCCAAGAAACACTTTACTTCTGAGACATTTCAAGAAGACAGCATCCCACGGACACAAG TAGAAAAAAGGAAAACAAGCCTGTATTTTTCCAGCAAGTACAACAAAGAAGCTCTTAGCCCCCCAAGACG CAAATCCTTCAAGAAATGGACCCCTCCTCGGTCACCTTTTAATCTTGTTCAAGAAATACTTTTCCATGAC CCATGGAAGCTCCTCATCGCGACTATATTTCTCAATCGGACCTCAGGCAAGATGGCCATCCCTGTGCTGT GGGAGTTTCTAGAGAAGTACCCTTCAGCTGAAGTGGCCCGAGCTGCCGACTGGAGGGACGTGTCGGAGCT TCTCAAGCCTCTTGGTCTCTACGATCTCCGTGCAAAAACCATTATCAAGTTCTCAGATGAATATCTGACA AAGCAGTGGAGGTATCCGATTGAGCTTCATGGCATTGGTAAATATGGCAACGACTCCTACCGGATCTTTT GTGTCAATGAATGGAAGCAGGTGCACCCTGAAGACCACAAGTTAAATAAATACCATGACTGGCTTTGGGA GAATCATGAAAAATTAAGTCTATCTTGAAGCTGCCTGTTTGAAGTTTCCCATGCGTCGCCTTGTGCTTCG GTTTCTAAGGGCCCTGTGGTGTGCAGTGAGCTCCCTTCCCGGCACGTGGATTTTTTTTTTCAGTTTGTTA GAAGCCAGTGTATGTCTTAAGCCTCTGCCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5356016 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5356016 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7107 same embryo
	EMAGE:7104 same embryo
	EMAGE:7108 same embryo
	EMAGE:7103 same embryo
	EMAGE:7106 same embryo
	EurExpress:euxassay_002606 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source