EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7107

Abhd2 abhydrolase domain containing 2 ( MGI:1914344)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002585. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002585_01	euxassay_002585_02	euxassay_002585_03	euxassay_002585_04

euxassay_002585_05	euxassay_002585_06	euxassay_002585_07	euxassay_002585_08	euxassay_002585_09

euxassay_002585_10	euxassay_002585_11	euxassay_002585_12	euxassay_002585_13	euxassay_002585_14

euxassay_002585_15	euxassay_002585_16	euxassay_002585_17	euxassay_002585_18	euxassay_002585_19

euxassay_002585_20	euxassay_002585_21	euxassay_002585_22	euxassay_002585_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7107_wholemount_strong.wlz

7107_wholemount_moderate.wlz

7107_wholemount_weak.wlz

7107_wholemount_possible.wlz

7107_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7107_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

homogeneous

strong expression: see section 07 08 09 15 16 17

submandibular gland primordium

moderate

regional

moderate expression: see section 05 06 07 08 16 17 18 19

lateral ventricle choroid plexus

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

4th ventricle lateral recess

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 16 weak expression: see section 03 04 05 06 07 17 18 19 20 21

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 05 06 07 16 weak expression: see section 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 15 16 weak expression: see section 07

cochlea

strong

regional

strong expression: see section 03 18 19 20 moderate expression: see section 01 04 22

not examined

regional

not examined expression: see section 01 02 21 22 23

neural retina

weak

regional

weak expression: see section 01 02 21 22 23

naris

moderate

regional

moderate expression: see section 10 11

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 13 14 weak expression: see section 08 15 16

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 10 11 13

stomach

moderate

regional

moderate expression: see section 01 02 03 weak expression: see section 04

genital tubercle of male

moderate

regional

moderate expression: see section 15 weak expression: see section 14

lung

strong

spotted

strong expression: see section 01 03 05 06 07 08 09 10 11 13 14 15 16 17 18 19 21 22 23 moderate expression: see section 20 weak expression: see section 04

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5227
Entity Detected:	Abhd2, abhydrolase domain containing 2 ( MGI:1914344)
Sequence:	sense strand is shown >T5227 ACCGGACTCCGGGCGGGTTGGACTGCTGGAGTCAATGAGAGAAGCTTTTAGGTTAATTTGAATATAAGAT CAGATCTGATTGGCCCACCTGGACACCCCTTCAAGGAAAGCCCGTATTCTCCGAGAGAGGAATAAGCGAC ATAGATCAAGATGAATGCCATGCTAGAGACCCCCGAGCTCCCCGCCGTGTTTGATGGGGTGAAGCTGGCT GCCGTAGCTGCCGTTCTCTACGTCATCGTGCGGTGTTTGAACCTGAAGAGCCCTACTGCCCCTCCTGACC TCTACTTCCAGGACTCCGGGCTCTCACGTTTCCTGCTCAAATCCTGTCCTCTTCTGACCAAAGAATACAT TCCACCACTGATCTGGGGGAAAAGCGGACATATCCAGACAGCCTTGTATGGGAAGATGGGGAGGGTGAGG TCACCACACCCTTACGGGCACCGCAAGTTCATCACCATGTCGGATGGTGCCACTTCTACCTTCGACCTCT TCGAGCCCCTGGCTGAGCACTGTGTTGGAGATGACATCACCATGGTCATCTGTCCTGGAATTGCCAACCA CAGCGAGAAGCAGTATATCCGAACCTTCGTTGACTATGCCCAGAAAAATGGCTACCGGTGCGCAGTGCTA AACCACCTGGGAGCCCTCCCCAACATTGAGCTGACCTCCCCACGAATGTTCACCTATGGCTGCACGTGGG AATTTGGAGCCATGGTGAACTACATCAAGAGGACATATCCCCAGACCCAGCTGGTCGTCGTGGGCTTCAG CCTGGGTGGTAACATCGTGTGCAAATACTTGGGGGAGACGCAGGCAAACCAGGAAAAGGTCCTGTGCTGT GTCAGTGTGTGCCAGGGGTACAGCGCACTGAGGGCCCAGGAGACCTTCATGCAGTGGGACCAGTGCCGCA GGTTCTACAACTTCCTCATGGCCGACAACATGAAGAAGATCATCCTGTCTCACAGACAAGCTCTCTTTGG AGACCACGTTAAGAAACCCCAGAGCCTGGAGGACACGGACTTGAGCCGGCTGTACACAGCAACATCCCTG ATGCAGATTGATGACAATGTGATGAGAAAGTTCCATGGCTATAATTCCCTGAAGGAATACTATGAGGAAG AGAGCTGCATGAGGTACCTGCACAGGATATATGTGCCTCTCATGCTGGTTAATGCAGCTGACGACCCCTT GGTGCACGAAAGCCTTCTAACCATTCCAAAGTCTCTCTCAGAGAAACGGGAGAATGTCATGTTCGTGCTG CCTCTGCATGGGGGCCACCTGGGCTTCTTCGAGGGCTCCGTGCTGTTCCCCGAGCCGCTGACATGGATGG ATAAGCTGGTAGTGGAGTATGCCAATGCCATTTGCCAATGGGAAAGGAATAAGTCCCAGTGCTCAGACAC GGAGCAGATGGAGGCCGAGTTGGAATGAGGTCTCTGGACTCGGCGTGCTCCAGCGGCCCTCCTCTGGAAC CCACGTCCCTTTGACGGCTGTTTCAGGTCTCCCAGTGACCTGGATCTGACCTCATACCATCAGTGGGGGG TTACCCATCATGCAACCTGTCTCAAGTAGGCGGCTCTCCCTGGGAGCTCCAGGCTATTTTTGTGCTTAGT TACGGGTTTTCTCCATTGCATCGTTAGCCATGGTGACAAGCTACAAGATTCTCACCCTTCTGTCCAGTTT CAGTATCTGATTGCTTTCACGCTGGTTAACATCTAGTTTTCCTAGTAAGGAGCGAGTCTGAACTATAGTT TTGCTTTGCCAATCAAAGGCCTTTTCCTGAGAACAGTGAAGGATATACGTCACTCTGTGATGGATGTATG CGCCGTACTAGAGTCCCCACCAAGAAGTCAGCATCCCTCGTGCTGAAGAAATTACTCAGCTTGGGAGTCT GAGAACCTGGGCCTTCCTGGGCCTGAGTTTTGCCTGTGAAACAAAGGAAATTCTCTGAATCAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5356667 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5356667 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7104 same embryo
	EMAGE:7108 same embryo
	EMAGE:7105 same embryo
	EMAGE:7103 same embryo
	EMAGE:7106 same embryo
	EurExpress:euxassay_002585 same experiment
	MGI:4822878 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source