EMAGE mouse gene expression spatial database

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EMAGE:7265

Suv39h1 suppressor of variegation 3-9 homolog 1 (Drosophila) ( MGI:1099440)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004374. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004374_01	euxassay_004374_02	euxassay_004374_03	euxassay_004374_04

euxassay_004374_05	euxassay_004374_06	euxassay_004374_08	euxassay_004374_09	euxassay_004374_10

euxassay_004374_11	euxassay_004374_12	euxassay_004374_13	euxassay_004374_14	euxassay_004374_15

euxassay_004374_16	euxassay_004374_17	euxassay_004374_18	euxassay_004374_19	euxassay_004374_20

euxassay_004374_21	euxassay_004374_22	euxassay_004374_23	euxassay_004374_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7265_wholemount_strong.wlz

7265_wholemount_moderate.wlz

7265_wholemount_weak.wlz

7265_wholemount_possible.wlz

7265_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7265_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5191
Entity Detected:	Suv39h1, suppressor of variegation 3-9 homolog 1 (Drosophila) ( MGI:1099440)
Sequence:	sense strand is shown >T5191 CCACGCGTCCGCGCCCGCGCGCGCTCTCTTCTCGCGAGGCCGGTTAGGCCCGAATGTTGTTAGCTGTGGA GAAAGATGGCGGAAAATTTAAAAGGTTGCAGTGTGTGCTGTAAATCTTCTTGGAATCAACTGCAGGACCT GTGCCGACTAGCCAAGCTTTCTTGTCCTGCCCTTGGTGTTTCTAAGAAGAATCTGTATGACTTTGAAGTT GAATACCTGTGTGATTATAAGAAGATCCGTGAGCAGGAGTATTACCTGGTTAAGTGGCGTGGGTATCCCG ACTCAGAAAACACCTGGGAGCCACGGCAGAATCTAAAATGTATACGAGTTCTTAAGCAGTTCCACAAGGA CTTAGAAAGAGAGCTTGTCCGACGACACCGCCGGTCAAAGCCACCCAGGCATCTGGACCCAAACCTAGCC AATTACCTGGTGCAGAAGGCCAAGCAGAGGCGGGCACTTCAGCGTTGGGAACAAGAGCTCAATGCCAAGC GCAGCCACCTGGGGCGGATCACCGTGGAGAATGAGGTAGACCTGGATGGCCCTCCAAGGTCCTTTGTCTA TATCAATGAGTATCGAGTTGGTGAGGGCATCACCCTCAACCAGGTAGCTGTTGGCTGTGAGTGCCAGGAC TGTCTGTTGGCACCCACTGGAGGCTGTTGCCCTGGAGCATCCCTGCACAAGTTTGCCTACAATGACCAAG GCCAGGTGCGACTGAAAGCTGGGCAGCCCATCTACGAGTGCAACTCCCGCTGTTGCTGTGGCTATGACTG CCCAAACCGTGTAGTCCAGAAAGGCATCCGCTACGATCTCTGCATCTTCCGCACTAATGATGGCCGAGGC TGGGGTGTCCGCACGCTGGAAAAGATCCGAAAAAATAGCTTTGTTATGGAGTATGTGGGAGAGATTATTA CCTCAGAGGAGGCAGAGCGGAGGGGCCAGATCTACGACCGCCAGGGCGCCACCTACCTCTTTGACCTGGA CTACGTGGAAGACGTATATACCGTGGATGCCGCTTATTATGGCAACATCTCTCATTTTGTCAACCATAGT TGTGATCCCAACCTGCAGGTGTACAACGTATTCATAGACAACCTTGATGAGCGACTACCCCGCATCGCAT TCTTTGCCACAAGAACCATCTGGGCGGGCGAGGAGCTCACCTTTGATTACAACATGCAAGTGGACCCCGT GGACATGGAGAGTACCCGAATGGACTCCAACTTTGGCCTGGCTGGGCTCCCCGGCTCCCCCAAGAAACGA GTCCGTATTGAATGCAAATGTGGGACAACGGCTTGCCGAAAATACCTCTTCTAGCCCTGAGAAGTCTGAG GCCAGACTAACTGAAGGGGCCTGAAGCCACCTTCCTCTCCTACAGCTACCCTCTTGTCAAGGATGACCAT CAATCAGAGCCTTGTCTGCCTCCACTTGTCCTCACCTACCCTAACCTGCTCTAGGGTCAGGGCTGTTGTG AGGACTAACTCCGGGTACCCTTTTCCTGTTCCTTTCCCCCTGTTCCAGGCCCATCAGGCATTGCACTTAA AACTCCCAGCCCCATTTTCAGAAACATATTTTTCACATCATGATTCCCTAGAGTTGGAATTCATGTCATA TAATGGAGGTCCAGATTGAGGAACTCGGCTGTAAAACAGATTCTTTGTTTTGGACAGCATCTCTGCAGCT CTATGTAGTAAGTCTGGTGTTTGGACCGTTAATCTTCCTGTCTCAGCCTTCCTCATGATGAGATTGTAGG TGTAAACCCAGCTAAGATTTTTGTTCTAATTGCATCTGCTTCTGCTTGGAGCTTGTGTGTGACCTGTTGC AGGCCTCTTCATTACTCTAATGGTATGAGGAAGCAACCCCCTGGCAGACAGACATCAGAGCTGAGATACC AGCCTAACATCAAGCTGGATCAGCAACCCCAGAGCCTTTGTACTCAGGAAAGAAAAGGCAATCTTCAGAG CTGGGAGATAAGGCTGGTTCCGCTCTTTGTGCTTTTGATGCTGGCTGGTATTAACCTTAAGACCTATAGG GTCTCAACAGTTGCAAGTCTGAAAAGTAGTTGCCCAAATGCCATCAGAATGGGGATGGAGTAAATACCTC TTTGAAAGCCCCACAGAAAGGTTAGAACTAAGTTTTACCATCAGGAAGTACAGTGCTGGACTTGCTGGAA ACCCAGCCTTGGCATTTGATGGCCACTAGAACTACTAGAAGCTGGAACCAAGATCTAGGTATTCTTTAGA TAGCACTTAAGACAGTAATGTGCATCGACTAGAAGGCGATGTGATGCCAGGCACTTGGTAGAGCACCTGG TCCATACAGATTGTCTCAGGGAAGCCTTGAAAACCACAAAGGTGGAGCCCAGAAAAAAGCCCATGTGACA GAAGGCAATGTCTAGGCCAAAAATACTTGTCAGCTCCAAGTATTCACCTGGGTCCACTTGTCTCAGTTAA CTGCCTAGAAATGTACAAAAGGCAAAGATTCTGATGGCTGCCTTGCCCCCTGCTTCCCCACCTCCAGGAA GCCTTTCCTGACTTCCTGTGCCCAGAGTGCCCTATGTGAAACTCTGTACCCTGCTACCAGATGCCAGGTC TGTGTGTGTATTTTGTATATATGTTTCCTGCCCATACTTCCCAGGCTGACCTTCAGGCATGGACTGAATC TGGTTCTCTCTGTACCCCTCAGCCCTCCCTAGCCTGGAGTGCACACCAATAAACTGTGTTGTTGAGTTAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5352230 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5352230 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7267 same embryo
	EMAGE:7268 same embryo
	EMAGE:7263 same embryo
	EMAGE:7266 same embryo
	EMAGE:7264 same embryo
	EurExpress:euxassay_004374 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source