EMAGE mouse gene expression spatial database

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EMAGE:7267

Anln anillin, actin binding protein ( MGI:1920174)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004352. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004352_01	euxassay_004352_02	euxassay_004352_03	euxassay_004352_04

euxassay_004352_05	euxassay_004352_06	euxassay_004352_07	euxassay_004352_08	euxassay_004352_09

euxassay_004352_10	euxassay_004352_11	euxassay_004352_12	euxassay_004352_13	euxassay_004352_14

euxassay_004352_15	euxassay_004352_16	euxassay_004352_17	euxassay_004352_18	euxassay_004352_19

euxassay_004352_21	euxassay_004352_22	euxassay_004352_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7267_wholemount_strong.wlz

7267_wholemount_moderate.wlz

7267_wholemount_weak.wlz

7267_wholemount_possible.wlz

7267_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7267_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 16 17

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5168
Entity Detected:	Anln, anillin, actin binding protein ( MGI:1920174)
Sequence:	sense strand is shown >T5168 CCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGAAAATTCAAATTTTGAACGGCCGTGGTAGCCTACC GACTCCGTGGGTGCGGAGGGCAGAGCCGACTGGGTGTGAGAGCGCCCGCCGCCTCGACTGCAGTCCTCCT CCAGGAGCTGCGCCGAGCCTGCACTCACTTCTTTCCTCTTCCTGAGTTTGAACCGTCGGACCCACCGTCT AGCCGTCCACTCGTGAGGCCTGGGGCGATGGACCCGTTTACCGAGAAGTTGCTAGAACGAACTCGTGCCA GACGAGAGAATCTTCAGAGAAAAATGGCTGAGAGGCCTACGGCAGTAGCGAGATCTGCCCCGCATGCGAA GAGAGGCAGAGAGCCACTTTCAGAAGCAAGTAATCAGCAGCAGCCCCTACCAGGGGGCGAAGAAAAATCT TGTACAAAACCATCACCATCAAAAAAACGTTGTTCTGACAAAATTGAAGTGGGAGCTCCGGACTTAGAAA ATACAGAACCTATTGATGTTGCAAAGCCCTGTTCTCCGATGCCTGCACCCCGGCAGGCGAAGCCCCCAGC ACCAGCTGCCATCAGCGAGTCTGTGGCTGCCCCAGCAGCCCTGCTCAGCGCGGACAGAGGGCTGAACTCA GGATCCGAAGCATCTGCAACCTCCTCAGTTAAAACTCGAATGCAAAGGCTTGCTGAGCAGCGGCGCCATT GGGATAGTGATCTCACAGATGATGTATCAGAAAGTTCATACTTTGCACCAGTGCCAACTGAGGACAAGGC TGCCTCACCTTCTAAGCCACCCATTTCAAATGCCTCAGCTACTCCAGTTGGGAGAAGGGGCCGTCTGGCC AACCTTGCTGCAACGATTTGCTCCTGGGAAGATGATGTAAGCCACTCATCTGCAAAGCAAAATAGTGTGC AAGAACAGCCTGGTACCGCTTGTTTATCCAAATCTTCCTCTGCAAGTGGAGCATCTGCTAGCATCAATAG CAGCAGTGTTCAGCAGGAAGCTACATGCTGTTCCCCAAGGGACGGCAATGCCTCTGTCAGGAAAGACCCA TCTTCAAATGCTGCCCATGGACCTTTGCTTAGCGCCTCAGTGTCCAGCTCTGTGAAAGCGTCTTCCCCTG TGACAGCTGCTACCTTCATCACTGAAAACCGTGAGGCACAAAATCCTGAGCTACTTCACAAAACTGCTAG TCCTCTGAAAACAGAGGCGCGGAAACCATGTGAGAAGCCAACTTTGTCCCAGGGAGCTCAGCCCAAAGAG GAGGCTAACAGAGAAGTTTGTCTACAGTCACAATCCAAGGACAAACTTGCAACACCAGGAGGAAGAGGAA TTAACCCTTTCCTGGAACGCTTTGGAGAGCGTTGTCAAGAACACAGTAAAGAAAGTCCGTCTTATAGAGC ATCTCATAAAACCCCAAATATCACTCCAAATACAAAAGCCATCCAGGAAAGATTATTCAAACAAAACACA TGCTCGTCTACTACCCATTTAGCACAGCAGCTCAAACAGGAACGTGAAAAAGAACTGGCATGTCTTCGTG GTCGACTTGACAAGGGCAATTTATGGAGTGCAGAAAAGAATGAAAAGTCAAGAAGCAAGCATCTAGAAAC CAAACAGGAAGTTCACTGTCAGAACACTCCACTCAAGAAACATCAAACTGTCGCAAGCACCCCATTGACT TCTGTAACAGATAAGGTGGCTGAAAATGAACCAGCAGTGAAGCTTTCTAGCACAGAGCCTGCAGGTTCCA CTGAAAGCGAAATGACAAAGTCCAGCCCTTTGAAAATCACGTTGTTTTTAGAAGAAGAAAAATCCTTAAA AGTAGCATCAGACCTGGAGGTTGAGCAGAACACTGAAGCAGTGCGTGAGGTTGAGATGAGTGTGGACGAT GAGGACATCAATAGCTCCAGAGTCATTAACGACATCTTCAGTGACGTCCTAGAGGAAGGGGAGCTGGATG TGGAAAAGAGCCAAGAGGAGATGGACCAAGTGGGAGCAGAAAACAGTGAGGAGCAGGAAGATGCGCTCAA TATCTCTTCAATGTCTTTACTTGCTCCGCTAGCTCAGACGGTCGGTGTGGTGAGCCTAGAGAATGTAATT TCTTCACCTCCGTCGGAATTGAGAGACTCTAACCTAAGCGCTGCAAGTCCTAAGCCCGGGAAATTCCAGA GAACCCGCGTCCCTCGCGCCGAATCTGCCGATAGCCTCGGTTCTGAGGACCGGGACCTTCTCTATAGCAT TGATGCATATAGGTCTCAAAGATTCAAAGAAACAGAACGCCCTTCCATAAAGCAAGTGATTGTTCGAAAG GAAGATGTTACTTCAAAGTTGGGTGAAAAGAAAAACGTATTTTCTGGTCAAGTTAATATCAAACAAAAAA TGCAGGAGCTCAATAATGACATAAATTTGCAGCAGACAGTGATCTATCAGGCCAGTCAGGCTCTCAACTG CTGTGTGGATGAAGAACACGGGAAAGGATCCTTGGAAGAAGCTGAAGCAGAAAGACTTCTTCTGATTGCA ACTGAGAAAAGAGCACTTCTGATTGATGAGCTGAATAAGCTGAAGAGTGAAGGACCTCAGAGGAGAAACA AGACCAGTGTCATATCCCAGAGTGAATTTGCTCCATCTAAAGGGTCAGTCACTCTGTCAGAAATCTGCTT GCCTCTGAAGGCAGATTTTGTCTGCAGCACTGCGCAAAAAACAGATGCATCAAATTATTACTACTTAATT ATGCTAAAAGCTGGGGCTGAGCAGATGGTCGCCACTCCATTAGCAAGTACTGCAAACTCTCTCAGTGGTG ACGCTCTGACATTTCCTACTACATTTACTCTGCATGATGTTTCCAATGACTTTGAAATAAACATTGAAGT TTACAGCCTGGTACAAAAGAAAGATTCCTTGGGCCCCGATAAGAAGAAGAAAGCCTCCAAGTCCAAGGCT ATTACTCCAAAGAGACTCCTCACATCTATAACTTCAAAAAGCAGCCTTCATTCTTCAGTTATGGCCAGTC CCGGAGGTCTCGGTGCTGTGCGTACCAGCAACTTTACCCTAGTTGGATCTCACACACTCTCCTTATCTTC TGTTGGAGACACTAAGTTTGCTTTGGACAAGGTACCTTTTTTGTCTCCGTTGGAAGGTCACATCTGTTTA AAAATAAGCTGTCAAGTGAATTCAGCTGTTGAGGAAAAGGGTTTCCTTACCATATTTGAAGATGTTAGTG GCTTTGGTGCCTGGCACCGAAGATGGTGTGTTCTCTCTGGCAACTGTATCTCTTACTGGACTTACCCAGA TGATGAGAGGCGAAAGAATCCCATAGGAAGGATAAATCTGGCCAATTGTATCAGTCATCAGATAGAACCA GCCAACAGAGAATTTTGTGCAAGACGCAACACTCTGGAATTGATTACTGTCCGACCACAAAGAGAAGACG ATCGAGAAACTCTTGTCAGCCAATGTAGAGACACACTCTGTGTCACCAAGAACTGGCTCTCTGCAGATAC TAAAGAAGAGCGGGATCTCTGGATGCAGAAACTCAACCAGGTCATTGTTGATATTCGCCTCTGGCAGCCT GATGCATGCTACAAGCCTGTTGGGAAGCCTTAAGCCGAGGAGCTTCTGCACCGTGAGAGACTTTGCTAGC TGTGTCTTCTTAAGAAGACAGTTAGAAGCAGCAGATTTGCAGGTTGTATTCTATGCTTTAAATATAAAAG GGTATGTGCAAATATTCACTACATATTGTGCAGTATTTATATCTTTTCTATGTAAAACTTCACCCAGTTT GTCTTGCATTCGTACATGTTTGACAGTCAAATACTAACAATATTCATGAGAATTGATATCCATGCTAAAT ATAACATTAAGAGTCTTGTTTTATAGAAACCTCACTAGCCAGTTATTCATGACAAAAACTATTATAATCA AGTTCTGATTTGTCCTTTGGAGCTGTGGGTTTGAAGGTATTAAGGTCTCAAACAGAAACATTTCAGGACA TGTTTAGTAAAGAGATGAGAAAAGGCAGCAAACACTAGTTTAAGCTGCTCAGAGCTGCTTTCCGCAGAGC TGTGGGCAGGACACCGTAACATTTGGGCCTGCATAGTCTATGCTGAAGGGTTAAGAGTCACACAGCTAGT GCTCACTCTGACCCTACGTGTGCAGTGTGGGGCACCTTCTCACAGTGCTCAGGCTTTACTAAAGAGTTCT TTCCATATGGCTTAGAGTCCTCATATTAACATTAGCTTTTCTCTTACAATAAGGACAAATGTAAGTTGAA GAGCACGTGCATAAGAGCCTGTACCAAGCTGCTGGGGCTACTCCTTTGCTTCCCAGAGTTCCTGTCTGAG GTCCTTTCATGTTTATCTATGACACTTTCTACAACTCTGTTCTGAGATCACTGAAAAATACAGTAAGCTA CGTGTTACCTTTAAAAGCCCAATGCCTCCTTGAAATTTAAAGATATTGAATGCATGGCTTCAGTTTTTAA ATAGTTTGTATTTCTTCTCTGACTTAATTATTGATGGTTTCATTTATAAACACTAAGTCTATCACTTGCC ATTATATTTCTTACTCATTTAAATGTGGATTTTCTTATGTATATTATAAAAGTATTTTATGACATAAATA AATAATTGTAAATTGTCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5346703 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5346703 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7268 same embryo
	EMAGE:7263 same embryo
	EMAGE:7266 same embryo
	EMAGE:7265 same embryo
	EMAGE:7264 same embryo
	EurExpress:euxassay_004352 same experiment
	MGI:4823140 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source