EMAGE mouse gene expression spatial database

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EMAGE:7266

Phf19 PHD finger protein 19 ( MGI:1921266)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004365. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004365_01	euxassay_004365_02	euxassay_004365_03	euxassay_004365_04

euxassay_004365_05	euxassay_004365_06	euxassay_004365_07	euxassay_004365_08	euxassay_004365_09

euxassay_004365_10	euxassay_004365_11	euxassay_004365_12	euxassay_004365_13	euxassay_004365_14

euxassay_004365_15	euxassay_004365_16	euxassay_004365_18	euxassay_004365_19	euxassay_004365_20

euxassay_004365_21	euxassay_004365_22	euxassay_004365_23	euxassay_004365_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7266_wholemount_strong.wlz

7266_wholemount_moderate.wlz

7266_wholemount_weak.wlz

7266_wholemount_possible.wlz

7266_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7266_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

weak

regional

weak expression: see section 13 14 15

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 13 14

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 17 weak expression: see section 11 12 16 18

telencephalon ventricular layer

moderate

regional

moderate expression: see section 17 weak expression: see section 03 04 05 06 07 09 10 11 12 16 18 19 20 21 22 23 24

medulla oblongata basal plate ventricular layer

weak

regional

weak expression: see section 13 14

midbrain ventricular layer

weak

regional

weak expression: see section 12 13 14 15

renal cortex

moderate

regional

moderate expression: see section 06 07 08 09 17 18 19 20 21 22 weak expression: see section 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5188
Entity Detected:	Phf19, PHD finger protein 19 ( MGI:1921266)
Sequence:	sense strand is shown >T5188 CCACGCGTCCGGGCTGACGCGCTGCCTCGCGAAGCTAATGGAGACTCAAGCTCTGGAACCAGGGACTCTG GAAGCCTTTGGTGCCACCAGTCCTAACAAGGGGGGCCTGTCTAAGACCAAAAAGAACTTCAAAGACTTGA TGTCTAAGGTGACAGAGGGACAGTTCGTGCTATGCAGGTGGACAGACGGGCTATATTACCTTGGCAAGAT CAAGCGGGTCAGCAGTCCTAAGCAAAGCTGCCTTGTGACTTTTGAAGATAATTCCAAATACTGGGTCCTG TGGAAGGACATCCAGCATGCTGGTGTTCCGGGAGAGGAGCCCAAGTGTGACGTCTGCATGGGGAAGACTT CAGGGCCTATGAACGAGATCCTCATCTGTGGGAAGTGTGGCCTGGGTTACCACCAACAGTGCCACATCCC CATCGCAGTTGATGCCAACTGGCCCCTCCTCACTCATTGGTTCTGCCGACGCTGCATTTTCGCACTGGCT GTGAGGAAAGGTGGCGCTTTGAAGAAAGGCGCCATCGCCAAGACGCTGCAGGCAGTGAAAATGGTGCTGT CCTACCAGCCGGAGGAACTCGATTGGGACTCGCCCCATCGCACTAACCAGCAGCAATGCTACTGCTACTG CGGCGGGCCTGGAGAGTGGTACCTTCGGATGCTACAGTGCTACCGGTGTAGGCAGTGGTTCCATGAGGCT TGCACACAGTGCCTTAGTGAGCCTATGGTGTTTGGAGACCGCTTCTACCTGTTCTTCTGCTCCGTGTGTA ACCAAGGCCCAGAGTATATTGAGAGGCTGCCCTTGCGATGGGTGGATATAGTTCACCTGGCTCTCTATAA CTTGGGAGTACAGAGCAAGAAGCGGTACTTTGACTTTGAGGAGATCCTGGCCTTTGTCAACCATCACTGG GAGCTCCTGCAGCTTGGCAAGCTCACCAGCACCCCCATGACAGAACGAGGGCCACATCTCCTCAACGCTC TCAACAGTTACAAGAGCCGGTTCCTGTGTGGCAAGGAAATTAAGAAGAAGAAATGCATCTTCCGACTGCG CATCCGAGTCCCGCCTGCCCCTCCAGGAAAACTGCTTCCCGACAGGGCGTTGATGCCAAGTGACAAAGGG ACCTCCGAGCTGCTTCGTAAGAAAGGAAAGAGCAAGCCTGGTTTGTTGCCTCAGGAACCCCAGCAGCAGA AAAGGCGAGTTTATAGAAGAAAAAGATCAAAGTTTTTGCTGGAAGATGCTATTCCCAGTAGTGACTTCAC CTCAGCCTGGAGCACAGACCACCACCTAGCCAGTATATTCGACTTCACACTGGATGAAATTCAGAGTTTA AAAAGTGGCAGCTCAGGCCAGACCTTCTTCTCAGATGTGGATTCTACCGACGCAGCCAGCACCTCGGGGT CTGCCTCCACCAGCCTCTCCTACGACTCCAGATGGACGGTAGGCAGCCGCAAGAGGAAGCTGACAGCCAA AGTGCACAGGCCCCTGCGAGCAAAGCAAAGGGCGGCGGAGCTGGAGGGGCGCTGCGCCTCAGACAGCAAT GCAGAGGGAGCTGTGGGTCCTGAGCAGCCGGATGAAGGCATCGACAGCCACACACTTGAAAGCATCAGTG GAGACGACTCGTCCCTGTCCCACCTCAAGTCCTCTATCACCAACTACTTTGGTGCAGCTGGGCGGTTGGC CTGCGGGGAAAAATATCGGGTGTTGGCGCGGAGGGTCACTCCAGAAGGCAAGGTTCAGTACCTGTTGGAA TGGGAGGGGACCACCCCTTACTGATTACCCTCAGAGGCTGCCCAGATCCCTGCAAACCAAAGGAGGGGCA GAAGAGGCCACTGGGTCCCTGTTTTCGCCAGGCTTGGGGTGGTGGTGGTGAAACAGGACATCCTGCCTGT GGCCCCTGCCTGCTGCCTGCCAGGCCAAGGCCTGTGTGCTGCTGTACCTGAGTCTCTGAGACCCCATTGG TATTCTCTCGTTGTCAATACTACACTCCACACCCCTCAAACTTTTTATTGGTTCCTCTGACAGTATCCTG GCTCCAACCCCTGGATCTGTTTTTTGTTGTCCCCAATTCTCCATCTTCTGCCCTCTCACCCCTTTCAGGT GTTTTGTGTGGACACACATCTTCCACGGGCATACACCCATCTGTCCCTCACTGGGCATGCTTCACTGGGT CCTCACTAGGACCACAGTGAGGGAAGGGAACCTTGGGGGTCTTCTGCTTTCTCACTAGTACCCCTCACCC CAGGAAGGTAGGGGGTGCCTGAGAAAGTTTAGGATGCCTAGGACCCAGAGTCTGGGGAGTGGTGAGCTGG GTCTCTGCAGAAGGGACAAGAATCAGGGTCTTTTATTGAGGAGGGGAGACTAAGGAAGTCAGGCAGGGCC AAGGTTTCCAGAAGTTTGCCTGTCCCAGCAACAACCCTTTACTGTTGTCAAGGATTGGGACCTGGCCCTG CTGCATAGACGTGTCTTAAACACACAGATCTGGGAGGAAAGGATGGGGAAGAAGACTAAGCCAGATTAAA TCCCATCCCAAACACTGTGTGTACAGCCACCACCTACTCCTGCCAGGGCCCCCTGGCCCCCCGTCCTTGG CCTCTTCCAGGGATACATGCTGCAGTTAAATGTGAGAACCAAACCCCTGCCTCCTCTGGCCTGTGAAGCA GCCTTGGCCCCACAGAGAAGCACAAAATATACCCCTCTCCTGCATGGCTCCTGCCTTGCCCTGGAGCTGA CAATCACTTTGGGGACAGGTTTGGAGTTTACAAGGGCCTGAATCGTTGGTTCCCTTCAGAAAGGAAGGCT TTGTCATCAATGGTACAAATAGAATTCTTAGAATGGTCAAGCTTAGCCGTGTCTGGGGAGAAACCAGGAG GTGGGCAGGGAGCCCTTACTTTGCCTACAGAAACAGAAATTGACTCACTCAGTTGGAGAAATGGAAAGAA CTGCCATTGAAGTGGGAGGCAAGGGCACCAGACTGAGACTAGCCTCCCTGACTGCTGCTGTAGCTTCCAG CTGGGGGTGGGAAGATGGACCACATGGTGACATCAGACTATACCAAAGGTGCTGATATTCCCATGACTAG GTCTGACCCCTTCACACCTTCCCGTGAGCATTAAGGAGGGTCCCTGCAGTAGGTTAGATGTCCCCTGCCT TAAACCTCAGCCACCATGGTGCCTTGCAAAGAAACCTCGCCTCTCATAGACACAGCTACCTACTTCCCTC TGCCTTCCTGTCTGGGCTCAGGCAGGGATAAGCAGATGGACACTGGGCCCAGTTGTCAGTCCCACAGCTG CCGCCACTGAGGTCCCAAAGTGGGGCCTGGTGGAAGGAGAGTTGGGAAGCCAACTCCTAGGCTTGTGGGA ACTGGGGTCAAATTTGTGGCTTCCTGTTGGCTTCGGTGAACATGGCAACCTCCAACACGTGTTTTCCAGG CCACTGGGGAAGCCAGAGTTAGGATGGCTATCTGTACATCTGCCCCTCCTCTGCTGTGCCCCAGAAGCTC TGGGCCTGAGGAGCAGGAAGGCAGGAGGGGTGCCCGTCCCGTTGGGGATCCTAGCACAAGGCCTGACCAG GTCCCTGGACTTCCCATGGCAGCATTTCACTCCTACAAAATAATAGCTGCATTACTGCCAACTGATGGTA TAACCCTGTGCACCTTTGAAAAGATTTTGGTTTTAAATTGCTGCTTTTAATAATATTTGTTATATTAAAG TTATAATTGATGTGTCTGGTTTACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5352251 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5352251 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7267 same embryo
	EMAGE:7268 same embryo
	EMAGE:7263 same embryo
	EMAGE:7265 same embryo
	EMAGE:7264 same embryo
	EurExpress:euxassay_004365 same experiment
	MGI:4827185 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source