EMAGE mouse gene expression spatial database

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EMAGE:7300

St3gal3 ST3 beta-galactoside alpha-2,3-sialyltransferase 3 ( MGI:1316659)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004417. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004417_01	euxassay_004417_02	euxassay_004417_03	euxassay_004417_04

euxassay_004417_05	euxassay_004417_06	euxassay_004417_07	euxassay_004417_08	euxassay_004417_09

euxassay_004417_10	euxassay_004417_11	euxassay_004417_12	euxassay_004417_14	euxassay_004417_15

euxassay_004417_16	euxassay_004417_17	euxassay_004417_18	euxassay_004417_19	euxassay_004417_20

euxassay_004417_21	euxassay_004417_22	euxassay_004417_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7300_wholemount_strong.wlz

7300_wholemount_moderate.wlz

7300_wholemount_weak.wlz

7300_wholemount_possible.wlz

7300_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7300_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6422
Entity Detected:	St3gal3, ST3 beta-galactoside alpha-2,3-sialyltransferase 3 ( MGI:1316659)
Sequence:	sense strand is shown >T6422 GCGGACCGCAGGATTGGTGGTCCCGCCGCTGCGTCCCCACCATGGCGGCGCCCGTGCAGCTTGCCGCGTC CTGGGCTCCCTCTGGGGTCCCCTCGGCTGTCCCCGCTATTCGCGCTGCAATTGCCTCCGCGCTCTGCGTG CGCCCTGGCGCCACCCGCCCCCGGCCTCCCTCTCTGCTGCGGTCATGTAGGAAATTGTGAGTCATGTGAA GATGGGACTCTTGGTATTTGTGCGCAATCTGCTGCTAGCCCTCTGCCTCTTCCTGGTCCTGGGATTTTTG TATTATTCTGCCTGGAAGCTACACTTACTCCAATGGGAAGACTCCAATTCATTGCTTCTTTCCCTTGACT CCGCTGGACAAACCCTAGGCACAGAGTATGATAGGCTGGGCTTCCTCCTGAAGCTGGACTCTAAACTGCC TGCCGAGCTAGCCACCAAGTACGCAAACTTTTCCGAGGGAGCTTGCAAACCCGGCTACGCTTCAGCTATG ATGACTGCCATCTTCCCCAGGTTCTCCAAGCCAGCACCCATGTTCCTGGATGACTCCTTTCGCAAGTGGG CTAGGATCCGGGAGTTTGTGCCACCTTTTGGGATCAAAGGTCAAGACAATCTGATCAAAGCCATCTTGTC AGTCACCAAAGAATACCGCCTGACCCCTGCCTTGGACAGCCTCCACTGCCGCCGCTGCATCATTGTAGGC AATGGAGGGGTCCTCGCCAACAAGTCTCTGGGGTCACGGATTGACGACTATGACATTGTGATCAGACTGA ACTCAGCACCTGTGAAGGGCTTTGAGAGGGACGTGGGCAGCAAGACCACCCTACGCATCACCTACCCTGA GGGCGCCATGCAGCGACCTGAGCAATATGAACGAGACTCTCTCTTTGTCCTCGCTGGCTTCAAGTGGCAG GACTTCAAGTGGCTGAAGTACATCGTCTACAAGGAGAGAGTGAGTGCATCTGATGGCTTCTGGAAGTCCG TGGCCACCCGAGTGCCCAAGGAGCCCCCTGAGATCCGCATCCTCAACCCATACTTCATCCAGGAGGCTGC CTTCACTCTCATTGGACTGCCCTTCAACAACGGCCTCATGGGCAGAGGGAACATCCCAACCCTTGGCAGT GTGGCAGTGACCATGGCACTACACGGCTGTGATGAAGTGGCAGTCGCGGGCTTTGGCTATGACATGAACA CACCCAATGCACCCCTGCACTACTATGAAACTGTGCGCATGGCAGCCATCAAAGAGTCCTGGACACACAA CATCCAGCGAGAGAAAGAGTTTCTGCGGAAGCTAGTGAAGGCACGTGTCATCACTGACTTAAGCAGCGGT ATCTGAGTGGGCCGGACACCGGGCCAGAGAGGCTCACGCCAGGACCAAGCAGCTGCCACCTGCACAGGAG CCTTCCCACCCAGAGAGGGACAGTGCCAAGAGACCCGAGGGCCAGTGAGGCCTTGGCAAGGCAACCAGAG CTGTGCCCACTGCAGGCCAGCTTCAGAGACCAGCACCCTGCCTTGCTGCCTGGGTCCATGAAGCCAGAGG GCCAGGAGGCCACTGGCTGTGGCCAGCAGGCCCAGCATGCAGGAGGTGGGACATTTGGTAGCAAGAATGC TACCAGAATCATTTCTCTAATCAGTGTTTGGTGTATTATCATTTTGTGAATTTGGGTATGGGGAGGGGAG GGATAATTTATTTTTAAATTAGATGTCACATTTGGTCCACTTGTTGTGCAGAAAGAGGCCCACTAGAGGG CCCATCAGACAGTGGTACCAGTGAACTCCCTGAAGGATGGAGGTGTGGTGACCATGCTTCAGGGACCGGG AGGTGGATAGCATCTCATGTCAGCCATCTCCAGCTCACGACACTCTTTGGCCTTCCTTGGGGAGAAGATG AAGTACTCCCTCTCACCAGCCACCAGTGTCCCACAGGGGAACCCCAGAGCCATCCCTTTAAAGCCAACAG GACAGCTCTTGGACAAGAGCAGAGAGAACTCAGGAGGCAAGGCCCAGCCCAGCCTGACGGGAATGAATGG CTCCGTCCATAGCTGTCCCTAATCCATGGGGCAGTGATCCTTCCTGAACTGTCAGATGGTCTGGGTTTCT TGCTGGGACTGCCAGCTCCATGGGTCCTACCCAGGACCAGAGGGAGGTGAGGCTCCAGGACCTCACCCAG GCTGCCGCAGCCAGCCGGGGCAGCTCTCACGGAAGCAATAAAGCTCTCCTCTGAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3584323 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3584323 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7302 same embryo
	EMAGE:7299 same embryo
	EMAGE:7301 same embryo
	EMAGE:7298 same embryo
	EMAGE:7303 same embryo
	EurExpress:euxassay_004417 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source