EMAGE mouse gene expression spatial database

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EMAGE:7303

Zfp7 zinc finger protein 7 ( MGI:99208)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004422. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004422_01	euxassay_004422_03	euxassay_004422_04	euxassay_004422_05

euxassay_004422_06	euxassay_004422_07	euxassay_004422_08	euxassay_004422_09	euxassay_004422_10

euxassay_004422_11	euxassay_004422_12	euxassay_004422_13	euxassay_004422_14	euxassay_004422_15

euxassay_004422_16	euxassay_004422_17	euxassay_004422_18	euxassay_004422_19	euxassay_004422_20

euxassay_004422_21	euxassay_004422_22	euxassay_004422_23	euxassay_004422_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7303_wholemount_strong.wlz

7303_wholemount_moderate.wlz

7303_wholemount_weak.wlz

7303_wholemount_possible.wlz

7303_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7303_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 11 12 14 15

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 17 18 19 20

thyroid gland

moderate

regional

moderate expression: see section 10 11 16

brain

moderate

regional

moderate expression: see section 01 07 09 10 11 12 13 14 15 16 17 18 19 20 21 weak expression: see section 02 03 04 05 06 08 22 23 24

adenohypophysis

moderate

regional

moderate expression: see section 10 11 12 13 14 15 weak expression: see section 16

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 18 19 20

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 16

cervical ganglion

moderate

regional

moderate expression: see section 08 09 17 18

thoracic ganglion

moderate

regional

moderate expression: see section 10 11

dorsal root ganglion

moderate

regional

moderate expression: see section 09 12 13 14 16 17 18 weak expression: see section 06 07 08 15

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 12 13 14 15 16 17

not examined

regional

not examined expression: see section 15

pharynx epithelium

moderate

regional

moderate expression: see section 13 14 15

tongue epithelium

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09

rectum

moderate

regional

moderate expression: see section 11

midgut

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20 21

lower jaw molar

moderate

regional

moderate expression: see section 08 09 19 20

palatal shelf epithelium

moderate

regional

moderate expression: see section 12 13 15 16

upper jaw molar

moderate

regional

moderate expression: see section 08 19 20

metanephros

moderate

spotted

moderate expression: see section 05 06 07 08 14 15 16 17 18

lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

trachea

moderate

regional

moderate expression: see section 12 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6428
Entity Detected:	Zfp7, zinc finger protein 7 ( MGI:99208)
Sequence:	sense strand is shown >T6428 GGCGAGCGGAAGTCGCCCGGGTGGGGCACAGTGGGATAAGCTTGCAAAGCCCTTTCCGGTGGCTCTCTTG TTGGGATCCACCACGCGCCGGGGCAGGTGTCTGACTCAGGACAAGCGGATCCCACCCTCCAGATCTCATG GAGGCGGTGACCTTTGGCGATGTGGCTGTGCACTTCTCTCGAGAGGAGTGGCAGTGTCTGGATTCTGGTC AGAGGGCCCTCTACAAGGAGGTGATGCTGGAGAACCACAGCAGCGTGGCAGGACTAGCAGGATTCCTGGT CTTTAAGCCGGAATTGATCTCCCGGCTGGAGCAAGGACAAGAGCCGTGGGTCCTTGATCTACAGGGAGCA GATGGGACAGAGGCACCAAGGATTTGCCAGACAGACTCCGCTATTAGAACTGATCGCAAGCAGACCTGTG AGTACACGAGCCTCCTCCAAAGGCAGATTCCTGGCTTTGGAGACAATTTGGATTCTAAGGTCTGGTCAGA GAATTGCCCAAGAAGCCTTGGGCTAAGCGTGAGTGGCTCACTTTTCCAGAAGCACCGTTTGAATAGCGAG GCCGTGATGCCCAAGAACTCCACAAAGGATGCTGTCCAAGAACGTAAGGAGCTGCAGGCCACCGATGTGG GTTATCGGCCTGATGACCAGAGAGACCACCTGAGCTCAAAACTGATTCGGCGTCAAAGCGTTCCTACCGG AGAGAACCGTTACCCATGTGAAGAGTGTGGCAAAGCTTTCAGGTGGCGCTCTCGCCTGAATCAGCACAAA CTGAGCCACACTGGAGAGAAATCTTATCAGTGCAATAAGTGTACAAAAGTCTTCGCTTCTAGCTCGCGAC TGATTCGCCATCAGAGAGCTCACACCGGAGAAAAGCCGTTTAAATGTGACCAGTGTGGGAAGCGCTTCGT CCTGGCCTCGGTACTCACTCAGCATCAGAGGATCCACACCGGAGAGAGGCCATTTAAATGTGCCGAGTGC GGGAAAGGTTTCCACCTGAGCGCAAAGCTGGTTCAGCATCAAAGAATTCATACTGGAGAGAAGCCTTACC GATGTGAGGAGTGTGGGAAAACCTTTGGTCAGAGCTCAAGCCTCGTCCATCATCAGAGAATCCACACCGG AGAGAGGCCCTTCATTTGTCAAGAGTGCGGGAAAGCCTTCTGCCAGCGCTCACAGCTCAGCAGACACCGG AGGACGCACACTGGAGAGAGGCCCTACTCCTGCCAGGAGTGTGGCAAGGCCTTCTGCCAGAGAGCAACTC TAGCCCAGCATCAGAAGATGATGCATACAGCGGAGAAATCTCAAATGCCGAGAGCCTCAGAGAGCCCAAG CCTTATTGCCTGTCAGGGAAATACCACTGAAGAGAAGCCATTTAAGTGTGAGCAGTGTGGCAAAGCTTTC AGGTGGCTCTCTCGCCTGAATCAGCACCAGGTGGTCCATTCTGGAGAGAAACCTTACCAGTGCAACAAGT GCTCGAAAGCCTTTGGCTGTACCTCACGACTGATTCGCCACCAGAGAACTCACACCGGAGAAAAGCCCTT TAAATGTGACGAATGTGGCAAGCGCTTCGTCCAGAGCTCACACCTTATTCAGCATCAGCGAATCCACACG GGAGAGAAGCCTTACGTGTGTGATGACTGTGGGAGAGCTTTTACCCAGAGCTCCAGCCTCATTTACCACC AGAGGATCCATAAGGGAGAGAAGCCATATAAATGCAGCCAGTGTGGCAAAGCCTTCAGCATGAGCACACA GCTCACCAGCCATCAGAGGACTCACACCGGGGAGAGACCTTATACGTGTAACGAGTGTGGGAAGACCTTC CGTCAAAACTCAACCCTTTTCCAACACCAGATAATTCATGTTAGAGTAAAGCCCTATGAGTGTAATGAGT GTGGAAAGGCCTTCAGCCGGAGCTCATACCTTATTGAACACCAGAGAATACACACTAGAGCCCAGGGGGG CCATGAATTTGGGGACACCCCTGAAAGTCCCACCGTTTTGAACCATAAAAAAGTGCACACTGTAAAAAAG TTGTACCAGTGTGACGACTGCGATAAGGTCTTCAGGTGGCGTTCGTACCTGATTATCCACCAGCGGATTC ACACAGGAGAGAGGCCTTATAAATGTAATGCCTGTGGCAAGGCTTTTCATCAGATCGCAAAGCTCACTCA GCACCAAAAACTTCACATGAGATAAATCATTTCCCTAAATGTCTTGGTTACTTCTCTCTTGGCTGTGATG GCACCATGAGTGACCAAGGCAGCTTAGACAAGAGTTCTCGTGGGGCTTACAGTGGAGCGTTAGTTTCAGA GATTGTTCACGGCAGTCTTGGCAGGGAGCAGGGCGGCAGGAAGGCCTGATGTTAGAGCAGTCGCTTAGAG CTCACATCGTGAGACAGCCAGTTACTAGGCAGAGAGATCTAACTGGAAATGGTGAGAGGTTTTAAAAAAC CTCAAAGCTGGCCTCCCAGTTACCTCCTCTAAAGAGGGCATACCTCCTAATAATTGCCAAACAGTTCCAC CAACTGAGGACCACATGTTGGAACATATGAGCCCCTGGAATTTGTTCTCATCCAAACCACCACACTAAAA GTAATGTAACTATGAATAAGCCAGAAGCCTTATTTAATTTGGGTAATTTATGCTATCAGAGACTTGAGGA TGCTAGGAAGATTCAGATTTGTTCTTAAAGAATAGTCTACTTAGGAAATGTTTTATTCGTTTCTGTAATA TTTTACCATTTACCCATTAAATAAAAGTGTGTGTCACACTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3584546 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3584546 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7302 same embryo
	EMAGE:7299 same embryo
	EMAGE:7301 same embryo
	EMAGE:7300 same embryo
	EMAGE:7298 same embryo
	EurExpress:euxassay_004422 same experiment
	MGI:4829333 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source