EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7394

Ankrd13a ankyrin repeat domain 13a ( MGI:1915670)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004641. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004641_01	euxassay_004641_02	euxassay_004641_03	euxassay_004641_04

euxassay_004641_05	euxassay_004641_06	euxassay_004641_07	euxassay_004641_08	euxassay_004641_09

euxassay_004641_10	euxassay_004641_11	euxassay_004641_12	euxassay_004641_13	euxassay_004641_14

euxassay_004641_15	euxassay_004641_16	euxassay_004641_17	euxassay_004641_18	euxassay_004641_19

euxassay_004641_20	euxassay_004641_21	euxassay_004641_22	euxassay_004641_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7394_wholemount_strong.wlz

7394_wholemount_moderate.wlz

7394_wholemount_weak.wlz

7394_wholemount_possible.wlz

7394_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7394_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7717
Entity Detected:	Ankrd13a, ankyrin repeat domain 13a ( MGI:1915670)
Sequence:	sense strand is shown >T7717 CTCCGGCCGGTCGGGCGGGCCCCCGAGACCCCGTCGGGTCCAGGCTGGTGGCAGGCGGGCGGTCAGGCCC GGGTGGCGGCCTCCTCGCCATGTCCTCGGCCCGCGACACCAGCAGCCGCTTCCCGCTGCACCTCCTGGTC TGGAACAACGACTACGAGCAGCTGGAGAAGGAGCTGCGGGACCAGAATGCAGAGGCCTTGGACCCCCGGG GTCGGACATTACTGCACCTTGCAGTTTCTCTGGGGCATCTGGAATCTGCTCGAGTCTTGCTGCGCCATAA AGCAGATGTGACCAAAGAGAACGGCCAGGGATGGACAGTCTTACACGAAGCTGTGAGCACGGGCGATCCC GAGATGGTGTACACCGTCCTGCAGCACCGAGACTACCACAACACGTCCATGGCCCTGGAAGGTGTACCCG AGCTGCTACACAAAATCCTCGAGGCCCCGGATTTCTATGTGCAGATGAAGTGGGAGTTCACCAGCTGGGT GCCCTTGGTGTCTAGAATATGCCCCAACGATGTCTGTCGCATATGGAAGAGTGGAGCCAAGCTGCGCGTG GACATCACCTTGCTGGGGTTTGAAAACATGAGCTGGATCAGAGGGAGGCGGAGCTTCATATTTAAGGGAG GAGACAACTGGGCGGAGCTGATGGAGGTCAACCACGATGACCGCGTGGTCACCACCGAACACTTCGATCT TTCCCAAGAAATGGAGCGCCTCACCCTGGACCTGATGAAGCCAAAAAGCAGGGAGGTGGAGAGGCGGCTG ACAAGCCCAGTCATCAACACCAGCCTGGATACTAAGAACGTTGCCTTTGAGAGAACTAAATCCGGATTCT GGGGCTGGAGGACAGATAAAGCTGAAGTTGTCAATGGTTACGAAGCCAAGGTTTACTCGGTAAACAACGT GAGCGTGATAACCAGGATCCGCACCGAGCACCTAACGGAGGAGGAGAAGAAGCGATACAAAGAGGACCGG AATCCACTGGAGTCTCTGCTCGGAACCGTGGAACATCAGTTTGGTGCTCAAGGGGACCTCGCTACGGAGT GTGCCACTGTGAACAACCCCACGGCCATCACTCCCGACGAGTACTTTGACGAAGACTTTGACCTGAAAGA CAGGGACATTGGAAGACCAAAAGAGCTGACAATTAGAACGCAGAAGTTTAAGGCTACGCTGTGGATGTGC GAGGAGTTCCCACTCTCCCTCGTGGAGCAGGTCATCCCCATCATTGACCTCATGGCTCGCACCAGCGCTC ACTTTGCCAGATTGAGAGATTTCATCAAATTGGACTTCCCTCCTGGATTCCCTGTCAAAATAGAAATCCC CTTATTCCACGTCTTAAACGCACGGATCACATTCGGAAATGTCAATGGCTGTAGCACTGCTGATGAGAGC CAGGGTGTGGAGGGGACCCCGGCCGAGGCAGTTTCCGAGGCCACAAACTTCGAGGTTGATCAGTCTGTCT TTGAAATCCCTGAATCCTACCACATTCAAGACAATGGCAGAAACGTGCACCTGCAAGACGAAGACTATGA GATCATGCAGTTTGCCATCCAGCAGAGCCTGCTGGAGTCCAGTAGGAGCCAGGACCTTTCAGGACCAGCT TCCAACGGAGGGGTCAGCCACACACACAGCTATGAAGCCCAGTATGAGAGGGCCATCCAGGAGAGTCTCC TAACCAACATGGAGGGCAGGTGCCCAGGTGGCCTCAGCGAGTCGAGCCGATTCGATAGCGACCTGCAGCT GGCCATGGAGCTGTCTGCCAAAGAGCTGGCGGAGCGGGAGCTCAGGCTCCAGGAGGAAGAAGCGGAGCTC CAGCAAGTCTTACAGCTGTCTCTCACTGAGAAATAGACATTGCCGTCCAGGGCTGCAGAGCAGAGGCTCT GGGCTGCACGTGCCATGCCAGTGAGGCAGACCCGGGGGGCCGAGGGCCTGCACTTACACAGCAGCCCCCC TTTCCTTATGTAGAGGTGCAGGACCAGGGACTCCCCAGAGCGGAGATCCGGGCGTCGGCAGAGCCCCATC TCCACACCGAGGGGAGGAGAGCATCTTTACCTTCCATTCGCTGTCCTGCCTGGCAGGTCACATTTTTTTT TTACCAGCTTTAGACAGAAATGCTGAGCCTGCAGGCTAGTAGATGCATCTTAGTCAGAAGTGACAAGCTA CTGCGGAGTCACGGTGCTTTTCCACAGGAGAACAGCAGCCTTTGTAGAGTGGGTACGAGCTGGAGACGTT CCTTCCTGTTCACCAAAGAAACGGGTTATGCTGACCTCTTTATTTCCCTTCCCGTAGGGCTCTTGCAGCA TTGCCATTGTGCCAGTCCCATTTCCACCTCGTTCCATGCCTTTTACCCCCAGCCAAGAGGATATGTACAG GAGCTATTTTAGATTATGGCTATTTGCATCAAATTCAGATATACAAATGACCACGGATGTTGCCTTAGCC TTAAAAAATTACTAACAGTTTTAAGCCACCTCACTCCACACACTGAGGACCTGATTTGAATCTGTAAAGG CAGTCCCTTTGGGAGCAGCATCCTCTGGGCTACACCAAAGGCAGCTAATCCTGTCCTTGGAAGAGCAGCT AGGGGGCCCCCCTGTGGCTGGGTTCTCAGAGCAGGCAAGCTGGCCACATCACTGGAAGAGCCCAGCCAGT GTGTTCTCCGCCTGCTCCCACTTAGGATTTCCAGCCTGGGGAGACCCAGCTGCACAGCCTGGCTTCATAG AGAGGACAGGACGAACTTGAAACAAGAAAGGGAGCTAACTTAGAGCAGAGGCTCTTCATCCAAAAGGGAA AAATGATGACTCCACACTGCTTTTTAGAGTTCAAATCAACATCTTTCTGGAAAAATATATTTTTTAACAG AATCTTTTTATTATTTTTAAAAGATATAAAACTGACTCCTCCCATCAGTAGCAATACAAGGTTATGCATT TTAACCAGACTTTCTCAGGCCTCTTTTGGATACCTTTAGTAGTTAACTATTTTTGTCTAACCCTCCTGTA AATACCTACTGCATAACAGCCCAACCCCTGGGGAACACAGCCGAGGGCACCTTGCCCCTCTCACCTGGCG CAGGCATCCACCCCCACAAGGCTGTGCTGATCACACGGCAAAACAGTACCTTTTCCTCTGTTCTCTGTCT GTTTCCTGTTATGCTTCTTTGGGGATAGGAAGAGAAGCCACCATTCCCAGGAATGTCTGTTTGATACAGT CAGCGTTGCCAGTAAAATGTTCTGAGACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6308996 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6308996 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7398 same embryo
	EMAGE:7397 same embryo
	EMAGE:7396 same embryo
	EMAGE:7399 same embryo
	EMAGE:7395 same embryo
	EurExpress:euxassay_004641 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source