EMAGE mouse gene expression spatial database

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EMAGE:7399

Ormdl3 ORM1-like 3 (S. cerevisiae) ( MGI:1913862)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004654. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004654_01	euxassay_004654_02	euxassay_004654_03	euxassay_004654_04

euxassay_004654_05	euxassay_004654_06	euxassay_004654_07	euxassay_004654_08	euxassay_004654_09

euxassay_004654_10	euxassay_004654_11	euxassay_004654_12	euxassay_004654_13	euxassay_004654_14

euxassay_004654_15	euxassay_004654_16	euxassay_004654_17	euxassay_004654_18	euxassay_004654_19

euxassay_004654_20	euxassay_004654_21	euxassay_004654_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7399_wholemount_strong.wlz

7399_wholemount_moderate.wlz

7399_wholemount_weak.wlz

7399_wholemount_possible.wlz

7399_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7399_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7715
Entity Detected:	Ormdl3, ORM1-like 3 (S. cerevisiae) ( MGI:1913862)
Sequence:	sense strand is shown >T7715 GCCGCGGCCCGGGTTCTCGGGCTCTCTCCCGGGCCCTCGACCTCCAACCCCGCGCGAGCGGCAGTCAAGA GGGGTGGCAGGATGAATGTGGGCACAGCACACAGTGAGGTGAACCCCAACACACGGGTGATGAACAGTCG CGGCATCTGGCTCTCCTACGTGCTGGCCATCGGACTCCTGCATGTCGTGCTGCTGAGCATTCCCTTTGTG AGCGTCCCTGTCGTCTGGACCCTCACCAACCTTATCCACAACCTGGGCATGTACATCTTTCTGCACACCG TGAAAGGGACGCCCTTCGAGACTCCAGACCAAGGCAAAGCAAGGTTGCTGACCCACTGGGAGCAGATGGA CTACGGGGTCCAGTTCACAGCCTCTAGGAAGTTCTTAACCATCACGCCTATAGTGCTGTACTTCCTCACC AGCTTCTACACCAAGTATGACCAAGTCCATTTCATACTCAACACTGTGTCCTTGATGACTGTGCTCATTC CCAAGCTCCCCCAGCTCCACGGAGTCCGGATCTTTGGAATCAATAAGTACTGAGGTTGTAGCCCCTTCCC CAGCCGGGTTGGCAGGGGAAGGGTGGAAGGCCTTTGCTGTGATGCTGAAGACAGGAGCCTCTGGACACTG CCAGAGATGGGGGTGTGCCCTGGGCCTGGCTTCCCCCTCGCTTCCCCAGTAGCCGACTTGGAGTAGCTTG TAGTGGGGTTAGGGTGGGGCCCTTGGGCTCTAACCCATTCTGAAGTTTTTGATCTTTTTCCTTTTGCCTT TTGAATAGAGATGCCATGGGGGTGGTCAGGAAAGAGGCTGGGCTCTCTGGCTGACTGTGGGCCTGAGAAA TTGGGACAGGAAGCCAGGAAAAGGCCCTGTTGGCTTGTTTACCCTCAGGCAGCCATAGCACTTTAGCCCC CAAAAAGGGGGGCAGAGGGACGGTACAGCTTCTGCATTGGTTGCCCTCATTCCTTCAGTGTGTGAAGCGA GACTCGCTGTTCTGTATGTATTAGAACCCTGCGCCGTGCTGCCTCTGCCGCCAGGCAGGGAGCAGGCGGG CTTTGTGGGCTTCCTGGCAACCCACGATGGGTAGGCTGTGGGGCAGGGGAGATTAGGAAAGGAAGATGGG TTGATGGGACACCTAGAGGTGGGGACAGGATGCTGCCACCTTGGTCCTGGGGAGAGGGGAGTTCTGAGCA AGGGTTGGGTGGGGATAGTAAGTTGGGTCTTACTTAGAATGGACAAGATAACTGCAGCCGATATGGATCA GTGTAAAAAAAAAAGAGGTCTGGGAAGGAGAAGCCAGACTCCGCCTCCTCTGAGATGTGGGCTAGGAGGA GAGCTGGCGTGGAGGGTTAATTTACCCACAGAACGATGCTGATAGGAAGGGAGGCCCCTGTGGGTTTGAA CTCCTGGGTTGGAATGAGGATATGCACAGGCTGGAAGGGCCTGTGAGTCCTCAGAAGCGGGTTCACTTCG ACACTTCCCTTAGTGGTGTGTGTGGGCGCGCGCGGAAGCTTTCTGAGAGGCTCTTCCTTAAGCGTTCAAT TCGTATTCTTGTGTAGATTGTGCTGGGGCAGTGCCTCTTGTGTGGCTTGCATGTGAATGGTCAGGAGGCC ACCTCCACATGTGGTCAGTGGCCTCCACAGTGCTACAGGATCTCTGCCCAAACCAGGCAGTAGTAGGGGC AGCTGCAGGCACTGTCCTCAGCCCAAAGAGAGGCAGGTCATGGAGCCTGAGGCTAGGCCGAGAGGAGCTG TCTCTCCACTCGCTGGGTCGACTGGGGCATGTTCCTTTGGAAACGCGTGGCTACCCAAGCCCTCTCCCAG GGAGGGGCTGGAGGGAGGTCACTCACCTGTTTGTCCCCCTCCCCCTCATTCTGAACCCAGTGTATCATAG GGAACTTTCACCTTCTGGTCTTTCTAAGCAAAGTGTGAATAGGATTTTTACTCCCTTTGTACAGTATTCT GAAAAAATGCAAATAAAGAGCAACATGTTCTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6308578 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6308578 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7398 same embryo
	EMAGE:7394 same embryo
	EMAGE:7397 same embryo
	EMAGE:7396 same embryo
	EMAGE:7395 same embryo
	EurExpress:euxassay_004654 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source