EMAGE mouse gene expression spatial database

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EMAGE:7396

Hnrnpa1 heterogeneous nuclear ribonucleoprotein A1 ( MGI:104820)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004646. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004646_01	euxassay_004646_02	euxassay_004646_03	euxassay_004646_04

euxassay_004646_05	euxassay_004646_06	euxassay_004646_07	euxassay_004646_08	euxassay_004646_09

euxassay_004646_10	euxassay_004646_11	euxassay_004646_12	euxassay_004646_13	euxassay_004646_14

euxassay_004646_15	euxassay_004646_16	euxassay_004646_17	euxassay_004646_18	euxassay_004646_19

euxassay_004646_20	euxassay_004646_21	euxassay_004646_22	euxassay_004646_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7396_wholemount_strong.wlz

7396_wholemount_moderate.wlz

7396_wholemount_weak.wlz

7396_wholemount_possible.wlz

7396_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7396_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7719
Entity Detected:	Hnrnpa1, heterogeneous nuclear ribonucleoprotein A1 ( MGI:104820)
Sequence:	sense strand is shown >T7719 CTGCCGAGGAAGCATCGCTGAAGGCTCTCGTAACTCTACCGTCATGTCTAAGTCCGAGTCTCCCAAGGAG CCAGAACAGCTGCGGAAGCTCTTCATCGGAGGGCTGAGCTTCGAAACAACCGACGAGAGTCTGAGGAGCC ATTTTGAGCAATGGGGAACACTAACAGACTGTGTGGTAATGAGAGATCCAAACACCAAGAGATCCAGGGG CTTTGGGTTTGTCACATATGCCACTGTGGAAGAAGTGGATGCTGCCATGAATGCAAGACCACACAAGGTG GATGGAAGAGTTGTGGAACCTAAGAGAGCTGTCTCAAGAGAAGATTCTCAGCGACCAGGTGCCCACTTAA CTGTGAAAAAGATCTTTGTTGGTGGTATTAAAGAAGACACTGAAGAACATCACCTACGAGATTATTTTGA GCAGTATGGGAAGATTGAAGTGATAGAAATTATGACTGACAGAGGCAGTGGGAAAAAGAGGGGCTTTGCT TTTGTTACCTTTGATGACCATGACTCTGTGGATAAGATTGTTATTCAGAAATACCATACTGTGAATGGCC ACAACTGTGAAGTAAGAAAGGCTCTGTCGAAGCAAGAGATGGCTAGTGCTTCATCCAGTCAGAGAGGTCG CAGTGGTTCTGGAAACTTTGGTGGTGGTCGTGGAGGCGGTTTTGGTGGCAATGACAATTTTGGTCGAGGA GGGAACTTCAGTGGTCGTGGTGGCTTTGGTGGCAGCCGTGGTGGTGGTGGATATGGTGGCAGTGGGGATG GCTATAATGGATTTGGCAATGATGGAAGCAATTTTGGAGGTGGTGGAAGCTACAATGATTTTGGCAATTA CAACAATCAGTCTTCCAATTTTGGGCCGATGAAGGGAGGAAACTTTGGAGGCAGGAGCTCTGGCCCTTAT GGTGGTGGAGGCCAGTACTTTGCTAAACCACGGAACCAAGGTGGCTATGGCGGTTCCAGCAGCAGCAGTA GCTATGGCAGTGGCAGGAGGTTCTAATTACATACAGCCAGGAAACAAAGCTTAGCAGGAGAGGAGAGCCA GAGAAGTGACAGGGAAGCTACAGGTTACAACAGATTTGTGAACTCAGCCAAGCACAGTGGTGGCAGGGCC TAGCTGCTACAAAGAAGACATGTTTTAGACAATACTCATGTGTGTGGGCAAAAACTCCAGGACTGTATTT GTGACTAATTGTATAACAGGTTATTTTAGTTTCTGTTCTGTGGAAAGTGTAAAGCATTCCAACAAAAGGT TTTACTGTAGACCTTTTTCACCCATGCTGTTGATTGCTAAATGTAATAGTCTGATCATGACGCTGAATAA ATGTGTCTTTTTTTTTTTTTTTTTAAATGTGCTGTGTAAAGTTAGTCTATTCTGAAGCCATCTTGGTAAA CTTCCCCAACAGTGTGAAGTTAGAATTCCTTCAGGGTGGTGCCAAGTTCCATTTGGAATTTATTTATGGT TGCTTGGGTGGAGAAGCCATTGTCTTCAAAAACCTTGATGTCGTTAAACTGCCAGTTACTATTGTAACTT TTAATGAGTTTCACCATTGAAAGGGTCATCCAAGCAAGGTCACAATTTGGTTATAAAATGGTTGTTGGCA CACCCTATGCAATATCAAAATTGAATAACGGTATCAGATAAAATAACAGATGGGAATGAAGCTTATGTAT CCATTATCATGTGTACTCAATAAACGATTTAATTCTCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6308746 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6308746 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7398 same embryo
	EMAGE:7394 same embryo
	EMAGE:7397 same embryo
	EMAGE:7399 same embryo
	EMAGE:7395 same embryo
	EurExpress:euxassay_004646 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source