EMAGE mouse gene expression spatial database

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EMAGE:7457

Golt1b golgi transport 1 homolog B (S. cerevisiae) ( MGI:1914214)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004588. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004588_01	euxassay_004588_02	euxassay_004588_03	euxassay_004588_04

euxassay_004588_05	euxassay_004588_06	euxassay_004588_07	euxassay_004588_08	euxassay_004588_09

euxassay_004588_10	euxassay_004588_11	euxassay_004588_12	euxassay_004588_13	euxassay_004588_14

euxassay_004588_15	euxassay_004588_16	euxassay_004588_17	euxassay_004588_18	euxassay_004588_19

euxassay_004588_20	euxassay_004588_21	euxassay_004588_22	euxassay_004588_23	euxassay_004588_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7457_wholemount_strong.wlz

7457_wholemount_moderate.wlz

7457_wholemount_weak.wlz

7457_wholemount_possible.wlz

7457_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7457_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 06 22 23 24 weak expression: see section 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7659
Entity Detected:	Golt1b, golgi transport 1 homolog B (S. cerevisiae) ( MGI:1914214)
Sequence:	sense strand is shown >T7659 AAGAGGTAATCCGCTCGGGCCCGGGCGGCGTCGCCGAGGCTCTTCTCCGGACCAGGCTTCGCAGCGTGGG CCCCGAAGCACCGCGGTCCTCGCCGCCGCCACCATGATCTCCCTCACGGATACGCAGAAAATTGGCATGG GCTTAACAGGATTTGGAGTGTTTTTCCTGTTCTTTGGAATGATTCTCTTTTTTGACAAAGCACTGCTGGC TATTGGAAATGTTCTGTTTGTGGCCGGCTTGGCTTTTGTAATTGGTCTCGAGAGAACGTTCAGATTCTTC TTCCAGAGGCACAAGGTGAAGGCCACTGGGTTTTTCCTGGGCGGTGTGTTCGTGGTCCTCATTGGTTGGC CTCTGATAGGCATGATCTTCGAAATTTATGGATTCTTTCTCTTGTTCAGGGGCTTTTTCCCAGTGGTCGT TGGCTTTATCAGAAGAGTACCTGTCCTCGGATCCCTCCTAAATCTACCTGGAATCAGATCATTCGTAGAC AAAGTTGGAGAAAGCAACAATATGGTATAACAACAAGAACTGAAGAACTTAAATACTGTATTCTTTATAA AGCCCTTTGGAAGAATACTCAGCACAAAATTAAAGTTCACGAACTAGGGTGTAAAGTTCCTTACAGAAGT TTAAAATGTACGCCCTCCAAAGTCCCAACAGCTGTGGCAGAAGCAGCGGCAGAAGCAGCGGCAGGCTTGT GAGGCCTGAGGTCCGAAGATGGGGAGGATGAAGCCTGTCCGATGCTTAGTGACTTGAAGGCTGTTTCCTA GTCTTGCTGCAGTATGTGAAACAGCCGTTCGTACAGAACCATGGTGTCTGTTACTTTCTTTTTTTTTTTA GAAGATTCAGGAACACCAATAGGCATTTGATTTTTTTATGATGTCCATTGTGGTGGCCCAGACATATCTA GGTATGCTGGGTTTCTAATAATGATGCCTGGATTGGATTATGTCATCTCCATGTATCGACCCAAGGAAGC CCAGTTTCATGTGTGAATCACTCCTTTTTTTTTTTTTTTTTTTTTTTTGTAAACATAGATAAAATAAAAC TTTTGTGGTCCTTTTGAATCTTAATATTTTGGAGCCAGATAAAAATCTGAACTACATAGTCATTTTTGAA ATACGCAGAGGTCATTCTGAGCTGTCACGTCCTCAGTCATAGCTGACCACGTTCTTTCTTGACTCTACAG CAGTCTGGAAGTGCAGATCTCTCGAGTGTGGCCTGGGTGTGTTCATACAATCTGTGCCGTGCAGAGGCCG AGCAGAGAGCGTTCTGACCATGGAGTAGCCTGCTGCAGAGGCTATTGGAGCAGTTTTGGGACCCTAAACC ATACACACCATATGAGTTCTCCATGCCATGGTGTAGTTTTTCTCTTTGGGTTCTTAATGAAATCTGGCCA TTGTCTCCCACCTTGCTTTCATAACTTACAGTGACGTTGAAGGTGTGCAACATGGTAAAGGTTGTTGTGT GACTGTGAGAGGGGCTGATTACTTCATCTTGATTTTTCCTCTTTTTTCAGCTTTATTGCAATATCCAAAA ATTAGCATGCACCGCATCTGCTTCCTTCGCTGTTGTCCACCTGAGTTAGGAAGTAAAAGCTTTTCGTTCT GACTAAATTGGCCATAGCTTCTTATAAACAAGACGCTGGGGAAGCATGACCTTCCCATCGTCTGCCGCTG GTTTGCCCACAGGAACCGTGATGTTTTCCTGTCGGACACCGGAGCACCTCGTCAGACACAGCACTATTTA TCTTACCACTAATGATTGCACTATTTATTTTTAACAGTCTCGAAGCTTTTTAATGCATAAAATCATAATT GAAATTATATTTATTTACAAGCCACTTCTATAAGACATTACAATGTTGTTTTTAGCTAAATTTCTCAGTG CCAGGAGAATTCCCAGCCTTGCCTATCTACAGAAGGAAGAACTGGTTCGTAGTGGGATGGCTCGTTCTTG TGGATCACTGTCGTGTTTGGTACTTGCATCTGACTTATGGTGTAACCTGTGAAAACTCGATAAGTGGAGA ATCCTACCTCAATAGCTCATGGGATAATAAAAGACCTGGCGTTGTGTCTGATGTTCCTCAAAACGTCACA CCTTTCCTGACGTGTCAATGCTATGCTTTGAGAACTGACCTGTGTAAGGTGCCCTGTATATTTATAATAG GCTTTGGCCTATATGATTGACAGTGGCTAGGTATCACTCGTGTCAGAATAAGAGTCTGCCATTTTACTCC TTTGTAGACATGACTCTATCAAAGTGGTTTTCACACTACAGCAAGCATCCCTTTCAGCAGTCTGATTTCA GAAAACACTGGACCTGGGAAGAATGAGAGGCTCATAGTCCTTAGTAAGGCTGAGGCAGCTTTCAGTTTAG GGTGAGACTAGTCTGCAGTGAGCCTAAACTGCTTGGCCTCCCTCGCGGCTTTTCTCAAGCAGAACCTCGA GGCTCTGCTTTTAAACAAAGCAGCCCTCAGACTTCTAGAAGTCCTATTGTGAGTTCCCTCAACCCTACCC CCTTCTTAAAGGAGCCTTCTGTCTTTAACAAAAAACAAACAGATACTTTGGTTATTCCCCCCAGGTGGAA GACAAGGTCCAGTCCTTAGCCTTAAGTGTTTCTGTCATGTTCAAGTGTATCTTCTGTAACAGAAACATAC TTGCAGTGTTTCTTTTCCCTTCTAATTCTCTGGAGTGCAGCTGCTGTAGAAGTCTCATGGTCAAATCATA TACTCTAACAATGGCGTATTGTAAATACACTTGTCTGACCTCAATAAAAGGGTACTTTTCTATTCAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30458855 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30458855 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7455 same embryo
	EMAGE:7454 same embryo
	EMAGE:7459 same embryo
	EMAGE:7458 same embryo
	EMAGE:7456 same embryo
	EurExpress:euxassay_004588 same experiment
	MGI:4825149 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source