EMAGE mouse gene expression spatial database

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EMAGE:7458

Maml3 mastermind like 3 (Drosophila) ( MGI:2389461)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004594. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004594_01	euxassay_004594_02	euxassay_004594_03	euxassay_004594_04

euxassay_004594_05	euxassay_004594_06	euxassay_004594_07	euxassay_004594_08	euxassay_004594_09

euxassay_004594_10	euxassay_004594_11	euxassay_004594_12	euxassay_004594_13	euxassay_004594_14

euxassay_004594_15	euxassay_004594_16	euxassay_004594_17	euxassay_004594_18	euxassay_004594_19

euxassay_004594_20	euxassay_004594_21	euxassay_004594_22	euxassay_004594_23	euxassay_004594_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7458_wholemount_strong.wlz

7458_wholemount_moderate.wlz

7458_wholemount_weak.wlz

7458_wholemount_possible.wlz

7458_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7458_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7660
Entity Detected:	Maml3, mastermind like 3 (Drosophila) ( MGI:2389461)
Sequence:	sense strand is shown >T7660 AAGAGGTAATCCCCAGGTTAAGCCCCACCTAGGCCTGGCAATGGCTATCTCCTTAATCCAGTCTCTGTGG CAGTATCTGGTCCAGGGTCAGGCTCTGTGGCAGGGCCCAGCTCTGACATGTCCCCAGCAGAGCAGCTCAA ACAGATGGCTGCCCAGCAACAACAGAGGGCAAAACTCATGCAGCAGAAGCAACAGCAGCAGCAGCAACAG CAGCAGCAGCAGCAGCAACAACAACAACAACAGCAGCACTCAAATCAGACTTCAAGTTGGTCTCCCTTAG GGCCTCCGTCAAGTCCATATGGAACAGCTTTTGCTTCAGAAAAACCAAATAGCCCAATGATGTACCCCCA AGCCTTTAACAACCAAAGCACTATAGTGCCTGCAATGGCAAACAGCCTGCAGAAGACGACCATGAACAAC TACCTCCCTTCCAATCACATGAATATGATCAGTCAGCAGCCAAATAACTTGGGTACAAACTCCTTAAACA AACAGCACAATATTCTCACCTATGGCAACACTAAGCCTCTGACCCATTTTAATGCGGACTTGAGTCCGAG AATGACGCCCCCTATGGCCAACCCCAACAAAACCCCCTTGATGCCCTACATCCAGCAGCCTCAGCAGTCG CAGCAGCCCCAGCCGCAGCCGCCCCAGCAGCAGCCACCGCCTCCCCCGCAGCTGCAGGCTCCCAGGGCGC ACCTGAGCGAGGACCAGAAACGCATGCTTCTCATTAAGCAGAAAGGAGTGATGAACCCACCCATGGCCTA TGCTGCGCTTCCTGCCCACGGTCAGGAGCAGCACGCAGTCGGTATTCCCCGGACCACAGGCCCTATGCAG AACTCTGTGCCCTCGGGGTCGGGCAGCATGGTCTCCGGAGCCAGTCCTGGGGGCCTTGGATTTCTAGGCA GCCAGCCACAGGCAGCCATCATGAAGCAGATGCTAATGGACCAACGGGCCCAGCTGATGGAGCACCAGAA GCAACAGTTCCTTCGGGAGCAGAGGCAGCAGCAGCAACAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAG CAGCAGCAGATCCTGGCTGAGCAGCAGTTACAGCAGCCGCACCTGCCCCGTCAGCACCTCCAGCAACAGC GGAACCCATACCCAGTGCAGCAGGTCAACCAGTTTCAAGGTTCACCCCAGGACATAGCAGCTGTGAGAAA CCAGGTGGCTCTCCAGAGCATGCGAGCATCGAGGTTGATTGCACAGAATGCGGGCATGATGGGAATGGGA CCCTCCCAGAACCCAGGGACGATGGCTACGGCAGCAGCGCAGTCAGAGATAGGTCTGGCCTCTTACAGTG CCCCTCCAACCAGCCAACCAGGAATGTACAACATGAACACAGGAATGACCCAAATGTTGCAGCATTCCAA TCAGAGTGGAATGGGCATCCCACACAACCAATCCCAGGGACCCAGGCCGCCAGCCTCCAGCCAGGGGGTC GGCATGGTGAGCGGCTTCGGTCAGAGCATGCTGGTGAACTCGGCTCTCTCGCAGCAGCACCAGCAGCTGA AAGGGTCTGTGGGCCAGGCCTTGCCTAGGCCCCAGGGCCCTCCAAGGCTTCAGAGTGTCATGGGAACCGT CCAGCAAGGAGCACAAAACTGGCAACAGAGGAGCTTACAAGGGGTGCCTGGGAGGACTAGTGGGGAGTTG GGACCATTCAACAATGGCGCCAGCTACCCATTGCAAGCCGGTCAGCCAAGACTGACCAAACAGCACTTCC CACAGGGGCTGAGCCAGCCCGTCATGGATGCTAACACTGGCGCAGTGAGAACCCTCAACCCAGCAGCTAT GGGACGTCAGATGATGCCAACCCTGGCAGGACAGCAGAGTGCCAGCCAGGTGAGGCCATTGGTCATGCCT GGTCTGAGCCAGGGTGTCCCTGGCATGCCAGCATTCAGCCAGCCCCCAGCACAGCAGCAGATAGCGGGTG GCAACTTTGCTGCGAGCAACCAGGGCCAGGCCTATGAGCGGACCCCTGCTCAGGACATGTCATACAGTTA TAGCGGCGAAGGTGTCGGGGCTGCCTTCCCTGGCCTCCCAGACAGTACAGACCTCGTGGACTCCATCATC AAAAGTGGCCCAGGGGATGAATGGATGCAAGAGCTTGATGAACTGTTTGGTAACCCCTAGTCAGGAGGAG CCCTAAGAGCTGTAGAAGCCTCACAAGTGGAAAGGAAACAGGATGGAGACCCATCCTTGTTTTTTTTGTT TTTTTGACCCACGTAAATTGAGAACATGACATCCGGCTGACAGTAGAAGGCACAGGTGTCCATCCCCAGC TCCGTGGGGGGCATCGTTCCACCTGACTTCCACAGAGCAATCAAGTAGAGAGGACGTGCCGATACCTGCC CGCAGAGAAGGGATGTAGAGAGCAGGTGGGGAAATCGAGGCAACGGGAGCTCCTCTGCTAAGCACCCCTG GGATCTTCTGGTCCAGAAGGAGCTGTTCCAGCCCAGAGGGACCATGGCCCAAGATGAGGTCCCCTTAGGC CTTCCTGCCTCAGGTCCCGAGACCTCAGCTTTGGAGATGAGAGATGGAGCCTCGGCCAGAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:30435466 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30435466 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7455 same embryo
	EMAGE:7454 same embryo
	EMAGE:7459 same embryo
	EMAGE:7457 same embryo
	EMAGE:7456 same embryo
	EurExpress:euxassay_004594 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source