EMAGE mouse gene expression spatial database

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EMAGE:7459

Fancb Fanconi anemia, complementation group B ( MGI:2448558)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004581. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004581_01	euxassay_004581_02	euxassay_004581_03	euxassay_004581_04

euxassay_004581_05	euxassay_004581_06	euxassay_004581_07	euxassay_004581_08	euxassay_004581_09

euxassay_004581_10	euxassay_004581_11	euxassay_004581_12	euxassay_004581_13	euxassay_004581_14

euxassay_004581_15	euxassay_004581_16	euxassay_004581_17	euxassay_004581_18	euxassay_004581_19

euxassay_004581_20	euxassay_004581_21	euxassay_004581_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7459_wholemount_strong.wlz

7459_wholemount_moderate.wlz

7459_wholemount_weak.wlz

7459_wholemount_possible.wlz

7459_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7459_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7663
Entity Detected:	Fancb, Fanconi anemia, complementation group B ( MGI:2448558)
Sequence:	sense strand is shown >T7663 AAGAGGTAATCCGTCCTTTGGTGCAACTGGAACGTGGCAATTTGGCAGCCTTGGAGAACAGGTTTTGGTT TGGTTTGGTTTTCTCCTGACCGCTCGCAGATAACCTGCATTGATTAAAGGCCTCAACTGGAATCCATTTG CAGTTGGAACTGGATCAACACAGAGACTTGATGCATTAGAGTGGCCGGCAAACAAGCAATGCCATTTAAT GAGCAAGCAAAGTTCTTGTGCTATCATGGGGAAATCCTTGTCTTTCAGTTTGTCCAGAGGAGAGCGTGCA GATTTAGAAATGCCTACAGATCCACTGGTCTTAGGTGTCAAAAGGATGATGTTTGACAGAGAAACTAGTA CATTCCTTCTGATATCCACTGTTTTTTTAAACATCAACGAAAAAGATTCTCATTTAAAAATTCTGTGTTG TAACTGTGTGTCCGATTTGAGAACTAGAATTAACCTTCCTTGCGTTTTGATACAATGTAGAAAGTACAAT AGTGAAGCTTTCAAATATTGTATCCTGCTTCTTCACAATCTCAACAGAGTGGAGAGACTTTTGAGTTTTG AACTAAACCATGCATTGGATGAGAACACAAAGATCTTTGACGGCCCTATAGTTTTCTGGCAGTATCTCAA CCAGTTCTTCTATATCTCTTCTGCAATTGGGAAAGTTACTACTATATCACTTATGCTTTCCTCTATTGAG TGGATCGGTGAGATTGAAAACTTTGGCCTAGGTTTCTTGGGGCTAGCAGAACCATCTGAGGATAAATGTA CCCAGAAGCTTTCAGAATCAGACTATGAATTTTCCAATTCCAGCCTTTGCGCATATGCTCTTAAAAGTCA AGAAATGTTAAGCAATGGTTACCTGATCCCTCTTGCTTACAGTACTATGGTAACACACGTGCATGTCTGG GCGGCGGAGATGGTTGACCACCAGTTAAGAACATCTCTGATTGCCCTGACTCGAAAGAATCAGCTCATTT TGTTTCAAAATGGCATTCCTGTAAGAGCATGTCAGCTTCCATTTCCAGGTCCCCGTTCAGTTCAGATTCT AGATGCAGGGAAAAGAAACCGATTTTTCATCGTGTCATTCCCGTCCAAGGCTTGTGCTGTTTCAGAAAAG AAATTTAAGGTTGTTGCTAAGTGGGAACAACTTAGCTTAGTACTGGTGAATGACTTTGCTGGAGTTGGAA CTGAACAAGTACTGGTCGTTTTTGAGGACTCTCTGGATGCAGATCAGCTGACCTCATTTACAGTAACAGA TTTTGTCAAAATATGGTATTCAACCAAACCACTGGATTGCTGTGAAGACCCTTTGGCTGAAGAGGAGCAT GAAAATTATTATTTGGTGCTTCCAGCACTGGAAGGCCAATTAGACAATTCTTTTATTTTTCTTAACAAAA TCCAGCAGCATATCTCTTTTAAGGATAAATTTATTGCAAAGTCTTGGAAGGCTCTTTTAAACGCAGTTTA TGGGAAAGGTGACAGTCTGCCAAGTGATGAAATGGTTAAGTTAGACCAGGACGGTCTTGTTCCTTTCTGT GATGAAGGTGAAGACAGTGTCCCTACCCCTGAGGAAAACTTACCAGACAATTTTCCAGAGCCAGAACATA TTGTAGAGCAGACATGGTGCCATGTACTAGATGATGACTTAGTTGTGGGCGCAAAAGTCACATCTTTGAA GGAATCTAATGAGATGACTTTATCACTGATAATGAATCAAGGCAACAGGTCCAGTTTTCATCTTATGAAG TGTCACAGTCAGGTATGGCTCTTGAAACACATGAAATGTGAAAGAATTCAAGAATGTACAGAAATATACC TTTATAAAAAGCTACGAAATTGTGGAGCACTCTTCTCCTGGGAGCAGAGAACAGCATCTGAAGGAATTTT AACAATATATTGCAGGAGCCAAGGAGTTTTGTTCCAGTGTCTCGACCATCTCATCAAAGTTCTCCCTGAA ATATGTTCCTTCAAATATCTGAAGGTCGAAAATGAGGATTTCTTAGTTGATCATTTGTCATCCACTTTGG AGGCAGAACTGGTTACCTTTTGTTCAGTTTCTACTTCTGCCTTCGAGTATGTTAGAGGTGGATATAACTG TAGAATAAGGAGAACAGATAATAGGGCCATGACATTTTTAGGGAGAAGGGCCAAAATCCGTCAGTCCAAG CGCAAAGTTCAGAGAGAAAGGATACTGAAGCACCTTAATATGACAGTAAATGGCAGCAGCTATGCGGAGA TGACTTTGGCGTTGGCTGAGATTCAGTTGAAATCAGACCTCATTGTAAAGACACTGGCCAATTTTGTAAT TGCACTTTAAAACTCATGATTTAAAATATTCCATAATTATTTGCCTCACTTGTTAGGTTTTGATAATATA AAGATTAAAGCTTATTATAATTCCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30460698 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30460698 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7455 same embryo
	EMAGE:7454 same embryo
	EMAGE:7457 same embryo
	EMAGE:7458 same embryo
	EMAGE:7456 same embryo
	EurExpress:euxassay_004581 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source