EMAGE mouse gene expression spatial database

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EMAGE:7583

Nipal2 NIPA-like domain containing 2 ( MGI:1924488)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004639. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004639_01	euxassay_004639_02	euxassay_004639_03	euxassay_004639_04

euxassay_004639_05	euxassay_004639_06	euxassay_004639_07	euxassay_004639_08	euxassay_004639_09

euxassay_004639_10	euxassay_004639_11	euxassay_004639_12	euxassay_004639_13	euxassay_004639_14

euxassay_004639_15	euxassay_004639_16	euxassay_004639_17	euxassay_004639_18	euxassay_004639_19

euxassay_004639_20	euxassay_004639_21	euxassay_004639_22	euxassay_004639_23	euxassay_004639_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7583_wholemount_strong.wlz

7583_wholemount_moderate.wlz

7583_wholemount_weak.wlz

7583_wholemount_possible.wlz

7583_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7583_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

stomach

moderate

regional

moderate expression: see section 01 02 03 04 weak expression: see section 05 06

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5284
Entity Detected:	Nipal2, NIPA-like domain containing 2 ( MGI:1924488)
Sequence:	sense strand is shown >T5284 GACAGGTGTAGGCACCGGGGCCCAGAAGGGAGCGGCGGCAACTGGGCCGAGCCCGGACCTCACCTGCGTG CCGCAGGAGCCAGGTGCCGGCGCCGGGTCGCCGGGCAGGAAGTGGCTGCGGCTGCGGGAGGACGGACGGG AGGGCCGCGCCCGAGTCCCAGCGCGTGGATTCAGTCCTCATGGCGGCTCCGGCGCGCACAGCCCCCGAGG ACGACGCCTCGGCCGTCCTAGACGAGCTGTCTCGGAACTTCACATACTGGGCACCAGGCCCGGGCAACGG CTCGCTGTCGAGCGCCTGGTATCGCAGAAACCAGATTCACCTTTTTGGAGTTTTGCTGGCCATTTTAGGA AACCTGGTTATCAGCATTTCCCTAAATATCCAGAAATATTCTCATCTTCATTTGGCCCAGAAAGAGCACC CAAAGCCATACTTTAAGAGTGTGCTTTGGCTGAGTGGAGTCCTGCTGACAGCCCTAGGAGAGACAGGCAA TTTCGCAGCCTATGGGGTTGCCCCCATCACTCTGATCGCACCACTGGGGTGCATGTCAGTTACAGGTAGT GCCATAATTTCTGTTATATTTCTAAAAGAAAATTTGAGAGCCTCGGATTTACTAGGTATGACACTAGCAT TTGCAGGAACATATTTATTGGTGAATTTTGCTCCAAATGTCACCCAGGCTATCTCAGCAAGAACAGTACA GTATTACTTTGTTGGCTGGCAATTCCTGGTCTATGTGATTTTAGAAATCCTAGTTTTCTGCATCCTTCTG TATTTCCATAAAAGGAAAGGAATGAAGCACATTGTGGTCCTGCTCACCCTGGTGGCACTCCTTGCCTCAT TGACTGTGATTTCTGTGAAGGCTGTCTCAGGCATGATCACTCTTTCTGTGACGGGTAAAATGCAGCTAAC TTATGCCATCTTCTACATCATGCTTGTCATCATGATAGCCTCTTGCGTTTTCCAAGTCAAGTTCCTGAAT CAAGCCACAGAACTCTACACAATGACCACAGTGGTGCCAGTCAACCATGTTTTCTTTACAACCAGCGCCA TCATCGCAGGCATCATATTTTACCAGGAATTCCTTGGTGCTGCTTTTCTCACTGTATTTATATATCTTTT TGGGTGCTTTCTGTCATTCCTTGGAGTATTTTTAGTGACAAGAAATAGAGAAAAGGAGCATCTACAACAG TCTTTTGTTGACCTTGGGGATATTCCTGGGAAACAAATGTTGGACAAAGTACAACCAGATTCAAATGGCT TATCCTATGGAACCTTGCCTGATGGAGGCGACTCAACAAGGGGCCAATGTGGAGAGAAGAAAGAATCCTA GGTTGACAAGAGGGATGGCTGTTGGCCTGCTATTCAATACCACCTTTTAAAGGATTGCACATGTTCGTTC TGTGTCAGCAGCTATTTCATGCTGACATGTGTGCACATTAGATTCACTTCTTTCCCCCACTGTGGACAAT CAGAGCCTTCCTAATCTCTAACAGTCTAATGCTTTCATGGAATGTAACTGCGAGTGCTCCCCACACCCTG CATCTCTCCCTAGCAATCATCTAGCTGGATCTTAACGAGAGCCTGGCTGCTGGAACAAGAATGTCACACA AAAATGTGCACTAGAGATCTGGCCATAGAAGGCAGAGGTTGCTTCTCCATGTGGCTTGCAGAATCTGCTT GTTGGCTTTAAAAACACCCTCTCACACTACAACCTTAGTCTTTAAAGGAAGATTCGATGTGCTTGTCCTT GGGCGCCTTCTATGAACCTCTCTTAAGGAACTGACTCCTTTTTCCCTGTTGTATGGCAAGTCACTGTCTC AGCCAATTCTGTTAAAAATCTTCCTTTGAAAATAAAGAGCCTCATTACAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5361417 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5361417 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7582 same embryo
	EMAGE:7581 same embryo
	EMAGE:7580 same embryo
	EMAGE:7585 same embryo
	EMAGE:7579 same embryo
	EMAGE:7584 same embryo
	EurExpress:euxassay_004639 same experiment
	MGI:4826715 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source