EMAGE mouse gene expression spatial database

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EMAGE:7585

Pcyt1b phosphate cytidylyltransferase 1, choline, beta isoform ( MGI:2147987)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004655. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004655_01	euxassay_004655_02	euxassay_004655_03	euxassay_004655_04

euxassay_004655_05	euxassay_004655_06	euxassay_004655_07	euxassay_004655_08	euxassay_004655_09

euxassay_004655_10	euxassay_004655_11	euxassay_004655_12	euxassay_004655_13	euxassay_004655_14

euxassay_004655_15	euxassay_004655_16	euxassay_004655_17	euxassay_004655_18	euxassay_004655_19

euxassay_004655_20	euxassay_004655_21	euxassay_004655_22	euxassay_004655_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7585_wholemount_strong.wlz

7585_wholemount_moderate.wlz

7585_wholemount_weak.wlz

7585_wholemount_possible.wlz

7585_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7585_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 18 19 moderate expression: see section 07

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 08 09 17 18 19 20 21 22 moderate expression: see section 07

vagus x ganglion

strong

regional

strong expression: see section 08 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 18 19 moderate expression: see section 07

trigeminal v nerve

strong

regional

strong expression: see section 09 16

spinal cord

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 16

not examined

regional

not examined expression: see section 16

dorsal root ganglion

strong

regional

strong expression: see section 08 09 11 12 13 14 15 16 17 18 moderate expression: see section 07

not examined

regional

not examined expression: see section 01 02 03 22 23

neural retina

strong

regional

strong expression: see section 02 03 22 23 moderate expression: see section 01

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 14 15 16 17 18

lower jaw incisor

strong

regional

strong expression: see section 10 moderate expression: see section 15 16

upper jaw incisor

strong

regional

strong expression: see section 10 moderate expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7723
Entity Detected:	Pcyt1b, phosphate cytidylyltransferase 1, choline, beta isoform ( MGI:2147987)
Sequence:	sense strand is shown >T7723 AAAGAAGGGAAGAAGAAGGGGGATGGGTAGGGAAGAGAAAAATACGTGACAAAGAAAACATTCTTTGCCT GGGAGGAGACTCCCAGAGGGTGTAGAGCTGGAGGGAGCATTCACTGGCCATGCCAGTACTTACCACTGAT GCTGAGTCAGAAACAGGTATCCCAAAATCCCTTTCCAATGAGCCTCCCTCAGAAACCATGGAGGAAATAG AGCACACATGCCCACAGCCTCGACTGACCCTGACTGCACCAGCCCCATTTGCTGATGAATCCAGCTGCCA GTGCCAAGCACCTCATGAAAAACTGACCGTTGCCCAGGCCCGCTTAGGAACACCAGTTGATAGGCCTGTT AGAGTGTATGCAGATGGAATATTTGACCTCTTCCACTCAGGTCACGCAAGGGCACTTATGCAAGCAAAAA CACTGTTTCCCAACAGCTACTTGTTGGTAGGAGTTTGCAGTGATGACCTTACTCACAAATTCAAAGGTTT TACTGTGATGAACGAGGCAGAGAGATATGAAGCTCTCAGACACTGTCGATACGTGGATGAAGTCATCAGA GACGCGCCATGGACTCTTACTCCAGAGTTTCTGGAAAAACACAAGATTGACTTTGTGGCTCACGATGATA TTCCATACTCCTCTGCTGGTTCTGATGACGTTTATAAGCATATCAAGGAAGCAGGCATGTTTGTTCCAAC ACAAAGAACAGAAGGCATCTCAACATCAGACATCATCACCAGAATTGTCCGTGACTATGATGTATATGCC CGGCGTAATCTCCAGAGAGGGTACACAGCCAAGGAGCTGAACGTCAGCTTTATAAACGAGAAGAAGTACC GTTTCCAGAACCAGGTGGACAAGATGAAAGAAAAGGTCAAGAATGTGGAAGAAAGATCAAAGGAGTTTGT TAACAGAGTAGAAGAAAAGAGTCATGACCTAATTCAAAAGTGGGAAGAAAAATCAAGAGAGTTTATTGGC AACTTCCTGGAACTGTTTGGGCCAGATGGTGCATGGAAGCAGATGTTCCAGGAGAGGAGTAGCCGGATGC TGCAGGCATTATCTCCGAAGCAGAGTCCTGTGAGCAGCCCGACCCGGAGCCGGTCCCCTTCTCGCTCTCC ATCTCCTACCTTCTCCTGGCTCCCAAACAAGACCTCTCCCCCCTCCTCACCCAAAGCAGCCTCAGCCTCC ATCAGCAGCATGAGCGAGGGGGATGAGGATGAGAAGTAAAGGTGCCTGGTCAACAAGAGCACACCACACA GGATGGGAAGGGCTCCCGGGATGCCTGGGTGGTGTTGAGAGCTGCAGCTCTGCTGGGAGACGGGAGTTCT GCCATGGGAGTTACTAGGGCCAGCTCTTTTTCCTTCTCCATTCAGTCTGGCCTGGGCTCAGCATGGAGGT TTCTGACCTGAAAACCCACACAGACCTTGTTAGCATCCTAGAAGCACCCTCACTTTAGCCCTGCTATGGA AAGGTGCAGAACACTCCATGAAGTGGGATGAGTTCTGCTTTGGTCCACACATCTATCTGTAGCCAATAAG CCACCCGCAGATGTGGCTGCTGCCTCGCCCTGCCCTCTCCAGTCCTCAAAGAGCCTTGCACGTTGCCCCC TGTCACCCATGCTCTCCCAGAATGAATTCCCTTCCCACAATGGGCAAAGTTCAGCCTTCCCAGTACAGCC ACTAAGGCTGGATGGAATGCACCAACCCTTTCCCATTTCAGGACCCTGCCTCAGACGCAGCTTCGCGTTG GTCAGATAGAAAAGTGTGACCCTTAATCTCTCACTCAAAGGACAGTTGTGTCAAGGTGAACTCCACCCAA GGCCTGCCATGCTTCTGGCTGTACATTTTCATCTTTGGCATGAGGAGACTGTTTTCCAGAAATTACCTGA ATTGATGGGAATCACAGCAAGGAAGCCCGTCTTATCTAAAAAAGAAATAACCACACAAACCTTCTTAGCA GGAGCTGCGCTGCTTTGGCTCCATGTATGGAAGATGAAAAATATCTCTTGGAGCTTCTTAAAGGATCAGC TCTTTCCATTTCTGACCACATCACAGTTCATAGACTCGAATAACAGTTTGATGTAACAAACACTTAGTAT ACCGATAGGGGGTGCTACCTGCACGTATCTGTTCTATGTATCCTCCAATATGCCACCCCAGGGTGTATCC CATATGCAATATGAATCTATGGTGGTGGTGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6309383 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6309383 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7583 same embryo
	EMAGE:7582 same embryo
	EMAGE:7581 same embryo
	EMAGE:7580 same embryo
	EMAGE:7579 same embryo
	EMAGE:7584 same embryo
	EurExpress:euxassay_004655 same experiment
	MGI:4827108 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source