EMAGE mouse gene expression spatial database

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EMAGE:7584

Olfr77 olfactory receptor 77 ( MGI:2153206)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016336. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016336_01	euxassay_016336_02	euxassay_016336_03	euxassay_016336_04

euxassay_016336_05	euxassay_016336_06	euxassay_016336_07	euxassay_016336_08	euxassay_016336_09

euxassay_016336_10	euxassay_016336_11	euxassay_016336_12	euxassay_016336_13	euxassay_016336_14

euxassay_016336_15	euxassay_016336_16	euxassay_016336_17	euxassay_016336_18	euxassay_016336_19

euxassay_016336_20	euxassay_016336_21	euxassay_016336_22	euxassay_016336_23	euxassay_016336_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7584_wholemount_strong.wlz

7584_wholemount_moderate.wlz

7584_wholemount_weak.wlz

7584_wholemount_possible.wlz

7584_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7584_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

single cell

strong expression: see section 11 12 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7722
Entity Detected:	Olfr77, olfactory receptor 77 ( MGI:2153206)
Sequence:	sense strand is shown >T7722 CACTATAACACTATGAGGTTGTAGTCTTTGTATGATACAAGAACTATAAATTTCATAGCATTCTTTCTAT GTCACAAAAGTATAGTTTCCACAAACCCTGTCTATCCAACTATCACTATTTCCTTTTCCCATTTTTGAAG TGTAGAATATTTTCCAATAAGAATTATTCCCTGGAAAATCTCTACTTTTTCCATCCTTTAATACAAGATT TTCTTCGTTTTGGATTGGGGCAACCCAGTAAGCATAACGATGTTTAGTTTAATGGAGAACTTTCTGGATT CTCTTCAGATCATACAGATCTTTGTCTTTTAATTTCAAGAACTTATTCAGACACTCCTTTTTTTTTAACT TTCAGAAAACAAAAAACCTGCATGGAAGCAGAGAACCATACAGCCATAGTACATTTCCTCCTTATTGGTC TTTCAGAAGATCCTAAAATTCAATCTGTTCTTTTTGGACTATTCCTATCTATGTTCCTGATTACCATGCT TGGAAACTTTCTCATTGTCTTAGTCACCAGCTGTGACTCCCACCTTCACACCCCCATGTACTTTTTCCTC TGCAACCTGTCATTTGTTGACATTTGTTTAACCTCCACTACTATCCCCAAAATGTTGGTGAACATTTACA TGCACACCATGAAGATATCCTACACAGAGTGCCTTACTCAAGTATATTTTTTTAATAATTTTCTTGGAAT GGATAATTTTCTACTGACCATTATGGCCTATGATCGTTTTGTGGCCATCTGTCATCCACTTAACTACACA GTCATCATGAACCCTCGAATCTGTGGCCTTCTGGTTCTTTTATCTTGGATCATTATGTTCTGGGTTTCCT TGATTCATATGCTGTTGATGAAACAACTGAACTTCTCTACTAGCACTGAAATCCCACATTTCTTTTGTGA ACTCACTGAGCTTCTCAGGGTGGCCCGCTCTGATACCCACATAAATAACATTTTTCTGTATTTGGTTACT GCAGTGCTAGGTATGTTTCCTGTCATTGGGATAGCTTTCTCTTATTTTCACATTGTCTCTGCTTTAATGA AAATGTCCTCCATTAAGAATAAGTATAAAGCCTTTTCCACCTGTGGGTCTCACCTCTGTGTGGTATCCAT GTTCTATGGGACAGGATTTGTAGTTCATCTCAGTTCTGCTGTAGCCCATTCATCCAAGAGAAACACAATC ACTTCAATAATGTACACTGTTGTCACCCCTATGTTGAACCCCTTCATCTATAGCTTGCGAAACAAGGATG TAAAGGGGGCCTTAGTAAGACTCCTTAGAAGAGTTAAATTTTGTCCTTGATGCATTACTGATACCAAAGA AAGATAATTATTGTGAGTACCAATATTCAAAGTCTTTTCATCCCTGGATCTTTGAAGTTTCTACTATTCT CATTGTTTTGGTTTTGTGAATATATTAATTGATTTCTCCCTAAATACCAATCAATTATCATCTTTTAACA TTTTTTTCCATAAAGTTTATGAAGAAATATAAAAGTTTGACATAAATTTTATGGACTTTTCTGATGTTTT GAAGTTTATCTACAGAATCTATGTAGGAACTAATCTGGTTTTCCTCAAAGTATATTGCTTAAGGAAAGGT GAGATGGTTCAACATGTGTGTAAAGAGCTTGCAGCTCTTTTAATATAAACTCAATAACTGATATTTGTGT AAAAGTGGAATCAGAGAATCAACTTCACAAATTTGTCCTCTGACCTTCACCAGCACCTTATGGCATGCAC AGGTTCACACATATGTACACATACAATAAAAATTATTTTTATAATTTAAAATAAATTGTTTGAATATTAC TATTTTCATCATAGTTTAGGTACATTTGTATCTTCTTATGAAACAATGTAAGGGAAATTACTGCAGCAAT ATAGCTATCACTGTATCAGGATACAGTAATTTTCTTCTTTCTTCTTTAAACATTTAAATCTACTCATATG GCAACATTTCTTTAAGAATTTTTCTCTCTTTTATGTAATATTTGAAAAGAAAGTACTAACTTGAAATGGA CAGCTGGAACTTGGCAATGACTACATGTTCATAAGGCATATATTCACTATCCCAATTTTTGTAATAACAA TTGAATTTTGAATATAAAGATAGAAATTTGAAAGAATTTTACCCACCTTACTTTTAATTACTTAGTAGCT AGAGTTATTCTGAAACATTGCTTCATTCTTTTAAAACCTTTGATCTTTTTCTTTTTATCTTATCTCATTT TCTATTTTATGTTTTTGCCCTCATTTCCTTTATCCTTATTATCTTCTTTCCTCTTGTTGTTTCATTGGTT TTCATTAACTAATCATTATGTCTATGAATGAGTATATTAGTTAATATATCAATTTTACACACTGATGGGC ATGTCACTAATTTCTATGACTTTTTATTTATAATATTTAAATAGATTTGTTTCAATAGTAACTATGCTAA CTATGAACAGAATAATTACATGAAGGTGTATCAGAATATATATTGTCTACTAGAAGTGATATCTGTGTTT ATGTATGTAGCATACTCTGCTCTGTTTGTTTATATAATTATAAAAATTTTCTTGTAATGCATGTGACAAA CAACTGTCCTTATCACACCTTGATTGTGATCAATTATTTGGAAAAATGTATAAGAATAAGAGAGTTGGCT CATATATATAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6309128 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6309128 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7583 same embryo
	EMAGE:7582 same embryo
	EMAGE:7581 same embryo
	EMAGE:7580 same embryo
	EMAGE:7585 same embryo
	EMAGE:7579 same embryo
	EurExpress:euxassay_016336 same experiment
	MGI:4826959 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source