EMAGE mouse gene expression spatial database

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EMAGE:7592

Rnf20 ring finger protein 20 ( MGI:1925927)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004658. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004658_01	euxassay_004658_02	euxassay_004658_03	euxassay_004658_04

euxassay_004658_05	euxassay_004658_06	euxassay_004658_07	euxassay_004658_08	euxassay_004658_09

euxassay_004658_10	euxassay_004658_11	euxassay_004658_12	euxassay_004658_13	euxassay_004658_14

euxassay_004658_15	euxassay_004658_16	euxassay_004658_17	euxassay_004658_18	euxassay_004658_19

euxassay_004658_20	euxassay_004658_21	euxassay_004658_22	euxassay_004658_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7592_wholemount_strong.wlz

7592_wholemount_moderate.wlz

7592_wholemount_weak.wlz

7592_wholemount_possible.wlz

7592_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7592_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7713
Entity Detected:	Rnf20, ring finger protein 20 ( MGI:1925927)
Sequence:	sense strand is shown >T7713 CCCAAGTGAATCCCTTGGAGTCCCTCTCGGGCCTTCGTGAAGAAGCGCTGGGAGCCGCGCGCTCCCTGCG GCGGGAGTTGCCAGAGCCCAGGCGAGGTCCAACCCAGATTGCTGCTTGCCATTGACGCCTCGTCCCGGAG TTTGGATCGGAAGCCGGCTGGATGTAGACCCCTGATGGCCGCGGTGCTCGCCATCTAGCATCCATTCCAC CTCCTTGTGAGTTTCGGAACCAGAGTCAGAAAAATATAACTGTCATCTTATACCAAGATGTCAGGAATTG GAAATAAAAGAGCAGCTGGAGAGCCGGGCACATCTATGCCTCCAGAGAAGAAGACAGCTGTTGAAGATTC AGGGACCACCGTGGAAACAATTAAGTTAGGAGGTGTCTCTTCAACGGAGGAATTAGACATTCGAACACTG CAATCCAAGAATCGTAAGCTAGCAGAAATGCTAGATCAGCGGCAGGCCATTGAAGATGAGCTTCGGGAAC ACATTGAAAAGCTGGAGCGACGCCAGGCCACTGATGATGCCTCACTGTTGATTGTGAACCGGTACTGGAG CCAGTTTGATGAAAACATCCGCATCATCCTTAAACGTTATGATCTGGACCAGGGTTTGGGGGACCTACTC ACAGAAAGGAAAGCCTTGGTTGTGCCAGAACCAGAACCTGACTCTGATAGCAATCAGGAACGCAAAGATG ACCGTGAGAGAGGGGATGGGCAAGAGCCAGCTTTCTCTTTCCTTGCTACTTTGGCTAGCAGTTCCAGTGA AGAGATGGAATCTCAGCTTCAGGAGCGTGTAGAGTCCTCCCGCCGTGCTGTGTCCCAGATTGTGACTGTC TATGATAAATTGCAAGAAAAAGTGGATCTCTTATCCCGGAAACTGAACAGTGGAGATAACCTGATAGTGG AGGAAGCAGTGCAGGAGTTGAACTCCTTCCTTGCACAAGAGAATGTGAGGCTTCAGGAGCTGACAGACCT CCTTCAGGAGAAGCATCACACCATGTCTCAGGAGTTCTGTAAGTTGCAGGGTAAAGTAGAGACAGCTGAG TCACGAGTGTCCGTCTTGGAGTCCATGATTGATGACCTGCAGTGGGATATTGACAAAATTCGAAAAAGAG AACAGCGTCTCAACCGGCATTTGGCTGAAGTCCTAGAGCGGGTGAATTCAAAAGGCTATAAAGTATATGG AGCGGGGAGCAGTCTCTATGGAGGCACAATCACCATCAATGCCCGGAAGTTTGAGGAAATGAATGCAGAG CTTGAGGAGAACAAAGAGTTGGCTCAGAACCGACATTGTGAGCTGGAGAAGCTTCGTCAAGACTTTGAAG AGGTCACTACACAGAATGAAAAGTTGAAGGTGGAATTGCGGAGTGCAGTGGAGGAAGTGGTTAAGGAAAC CCCAGAGTATCGCTGCATGCAGTCACAGTTCTCTGTCCTGTATAACGAGAGTCTGCAGTTGAAAGCCCAC TTGGATGAAGCTCGGACCCTGCTTCATGGCACCCGGGGTACTCACCAGCGCCAGGTTGAACTCATTGAGC GAGATGAGGTTAGTCTGCATAAGAAGCTGAGAACTGAAGTCATTCAGCTGGAGGATACCTTGGCCCAGGT TCGCAAGGAGTATGAGATGTTGAGGATAGAGTTTGAGCAGACCCTTGCTGCCAATGAACAAGCAGGCCCC ATAAACCGGGAGATGCGGCATCTCATTAGCAGCCTCCAGAATCATAACCACCAGCTGAAGGGTGAAGTCC TAAGGTATAAACGGAAGCTGAGAGAGGCGCAGTCTGACCTGAACAAGACACGTCTGCGCAGTGGCAGTGC CCTCCTGCAGTCTCAGTCTAGTACTGAGGACCCCAAGGACGAGCCCACAGAGCTGAAGCAGGATTCTGAA GACCTAGCCACTCATTCCTCAGCGCTGAAGGCATCTCAGGAGGATGAAGTTAAGTCCAAACGGGATGAAG AAGAGAGAGAACGAGAAAGGAGGGAGAAAGAAAGAGAGCGAGAGCGAGAACGAGAAAAGGAAAAGGAGAG AGAACGAGAGAAACAGAAACTGAAAGAGTCAGAAAAGGAGAGAGATTCTGTTAAGGATAAAGAGAAAGGG AAACATGATGATGGAAGGAAGAAGGAAGCAGAAATTATCAAGCAGCTGAAGATTGAACTTAAGAAAGCAC AAGAGAGCCAGAAGGAGATGAAACTACTGCTGGATATGTACCGCTCTGCCCCCAAGGAGCAGAGAGACAA AGTGCAGCTAATGGCAGCTGAGAAGAAGTCAAAGGCAGAGTTGGAGGATCTCAGACAAAGACTCAAGGAT CTGGAAGATAAGGAGAAGAAAGAGAACAAGAAAATGGCTGATGAGGATGCCTTGAGGAAGATCCGGGCAG TGGAGGAGCAGATTGAATACCTGCAGAAGAAGCTGGCTATGGCCAAGCAGGAGGAAGAGGCTCTCCTGTC TGAGATGGATGTCACAGGACAGGCCTTTGAGGACATGCAGGAGCAGAACATCCGTCTGATGCAGCAGTTG CGGGAGAAGGACGATGCAAACTTCAAGCTCATGTCAGAGCGAATCAAGTCTAATCAGATCCATAAGTTGC TTAAAGAGGAAAAGGAAGAGTTAGCTGATCAGGTTTTGACACTGAAGACTCAGGTAGATGCCCAGTTACA GGTAGTAAGAAAACTGGAAGAGAAGGAGCACCTACTTCAGAGTAACATTGGCACAGGGGAGAAGGAGCTG GGTCTTAGGACTCAAGCCTTGGAGATGAATAAACGCAAGGCAATGGAGGCGGCCCAGCTTGCAGATGACC TCAAAGCACAGCTAGAACTGGCTCAGAAGAAGCTCCATGATTTCCAGGATGAAATCGTGGAGAACAGTGT CACCAAAGAAAAGGACTTGTTCAATTTCAAACGAGCACAGGAAGACATCTCTAGGCTTCGAAGAAAGCTA GAGACCACCAAGAAGCCAGACAATGTGCCCAAGTGTGATGAAATTCTGATGGAGGAGATAAAGGACTACA AGGCACGGCTGACCTGCCCTTGCTGTAACATGCGTAAAAAGGATGCAGTACTTACCAAGTGTTTTCACGT TTTCTGCTTTGAGTGTGTGAAGACGCGCTATGACACTCGCCAGCGCAAGTGTCCCAAGTGTAATGCTGCT TTTGGTGCCAATGATTTCCATCGCATCTACATTGGCTGATTGCAGTCAAGAAAGAGGACTGGCTAGGCAA GCACTTACTCATTAATCACCAGGCCTCTACCTCCTCTCTCCTAGACAGCTGGCCATCTCCCTTGTTCCCC AGACTTTGTCCGGGTTCTCTTCGCCAGTAGCGAGAGAACATAGTGAGGAAGACTGCAAGTCTTGGGACTT AGAATGAACTAGAAATTACTAGCGTTTGTCTTCCCTTCAGCTTTCTTTGTGTCCTTCTTTCGGATTTTTC AGCATTTTCTTCTTGGGCCTAGCCTATAAACTGGTTTTGCCCACTCCTCTTGAAGAAGTCAGGTTGATAG TTTGGGCACTGAGAAGGGAATGTGGGTAGAGTGAACATATACTATTATGGAGGAGTGAATTTTTGATAAG TTATCTATGGGCCCAAAATTCTTGATTTGGGTAGTAAACCTTTCTAAACTTAGGTTATAAAATGGTATAG TTGTGTTAGTCTCTCCTGGAAGGTTTGAAGCCTTAATGAAATTATATCCAGGATACTTCATTCTATTTCT CACTTCTAGGAGACTGACTTTTTGGGTCAGTTGGACTTCTTGCTAAAGTTATTTATGTTTTAGGTATTCA AATATTAATAGTTTTTTTTAATTTCTTAAAGCCAGAATAATTTCAAATGTGATAGTCCCTATGGTGTGTG TTTTTTTTTTTTAATATACATCTTTTCTGAAATCAGAATTAGTATTAAGAAGTAACAGGAATGCATTGAT TGTGAAGAGATCTCGGTAGTCTGGGAATGAGATCCAGGGCGATGTTGGGGAATTATGAATTACTGTCCCC TCCTCTCCAGAGGGAGTCTTTGTTCTCTGTGTTCCTGAAGATTCTCAGTAATGTTTCTGTAGGATTTTAA TATTCCATGTGCTTCAATTAAAGCAGGATTCAGGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6308280 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6308280 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7594 same embryo
	EMAGE:7593 same embryo
	EMAGE:7589 same embryo
	EMAGE:7591 same embryo
	EMAGE:7590 same embryo
	EurExpress:euxassay_004658 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source