EMAGE mouse gene expression spatial database

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EMAGE:7613

Rnf216 ring finger protein 216 ( MGI:1344349)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004683. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004683_01	euxassay_004683_02	euxassay_004683_03	euxassay_004683_04

euxassay_004683_05	euxassay_004683_06	euxassay_004683_07	euxassay_004683_08	euxassay_004683_09

euxassay_004683_10	euxassay_004683_11	euxassay_004683_12	euxassay_004683_13	euxassay_004683_14

euxassay_004683_15	euxassay_004683_16	euxassay_004683_17	euxassay_004683_18	euxassay_004683_19

euxassay_004683_20	euxassay_004683_21	euxassay_004683_22	euxassay_004683_23	euxassay_004683_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7613_wholemount_strong.wlz

7613_wholemount_moderate.wlz

7613_wholemount_weak.wlz

7613_wholemount_possible.wlz

7613_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7613_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8665
Entity Detected:	Rnf216, ring finger protein 216 ( MGI:1344349)
Sequence:	sense strand is shown >T8665 GCCGGAAGGAACGCAGTAGCGGCCTCGGTGGCGGCGGCGGCGGTCGCAGCGGCGGCGGTCGCAGCGGGGG AGCTTTTCCAGCGGCTGCTTCTTCAGAACAGATGATCACCATGTTTGGTTACTAGCAATCACACATGTTT GTATCTGAATTTGAAAATGGCAGAGGGAAACAACAAAGAGGAGGTCATTCACTTGAACAACTTTCCCTGC CACCGGGGAAAGGAATGGATGGCTGTCAGAGAGGGGCCCATCACCATATCTGACTCCTCTGATGAGGAAG GGATTCCAATGCTGGTTACCCCAGCTACAGAGCAACAGGAAGACGACCTGGATGATGATGTCATCCTGAC AGAAGATGATTCTGAGGATGAATATGGTGGATTTCTGGATCTTGAGAGTGGCAAAAAAGAGGGAGAAGCC AAACCTGGACCAAGCAGTAAGCAGACAGCAGACGATATTGTCAACCCCAGATTGGAGCAGAAAGTCATTA TATTGGGAGAAAATGGTCTTCTTTTCCCAGAAAGTGAGCCTTTGGAAGTTCAGAACCAATCATCCGAAGA CTCAGAGACAGAGCTCTTATCAAATCCTGGAGAGCCAGCTGCCTCAGTAGATGACCAGCTAATTGGAGAA GAGTACTGGCTTGACCATCCATACTTCCAGGCTCCGAATCCACAGCCTCAGGAAAGAACAAACCAGGTTG TGCCCCAAGAGCGACATTCTGAATCAGAAATGGGCCCAATGTTTTTCCGCCATGATTTCCCAGAGCCAGC TTTTCCAAGGCCAGAACCCCAGCAGGAGGGGATTCCAGGCCCTGCTTCTCCACAGCCAGCCCATCCTCTA GGAGAGCTTGAAGACCAGCAGTTAGCAATTGATGAAGACCCTGGGCCAGCCTTCCCCCTGTCAGGACCTC AGGAGGCCAACTTGGCAAACATGTGGGAACAAGAAGCTGCTGAGGTAGATCAAGACCTCATTCCACTGTT AGTGAAAGAAACGGAAGCAAGATTTCCAGATGTAGCAAGTGGATATGTTGAAGAAATAATTCATTTGAAA AATTACTATGATTTGAATGTACTTTGTAATTTTCTTCTGGAAAACCCAGATTATCCAAAGAGAGAAGACC GGCTTATTATCCACCCCAGCAGCAGCCTGCTTGCCAGCCAGGATGACGCGAAGTTGCCTAAAATAGACTT TTTTGACTATTCCAAATTGACTCCACTTGACCAGCGCTGCTTCATCCAAGCTGCTGACCTCCTGATGGCT GATTTCAAGATGCTTAGCAGCCAGGACATCAAGTGGGCCCTTCATGAGCTCAAAGGACACTATGCAATCA CCCGAAAGGCCTTTTCCGATGCCATTAAGAAATGGCAGGAGTTGTCACCTGAAACCAGTGGAAAACGGAA AAAGAGGAAAGAAATGAATCAGTATTCTTTCATAGATTTCAAGTTTGAACAAGGAAACATAAAAATAGAA AAGAGGATGTTCTTTCTGGAAAACAAGCGTCGACACTGTAGGTACTATGACCATCAGGCCCTCCTTCCAG CTGTGAAACAGGAGCAGGAATTCTATGAGCAGAAAATTAAAGAGATGGCAGAGCATGAAGACTTCTTGCT CGCCCTGCAGATGAATGAAGAACAGTATCAAAAGGATGGCCAGCTGATTGAATGCCGCTGTTGCTATGGG GAGTTTCCATTTGAGGAGCTGACACAATGCGCCGATGCTCACTTGTTCTGCAAAGAATGTCTCATCAGAT ATGCCCAGGAGGCAGTGTTTGGATCTGGAAAGTCAGAACTCAGCTGCATGGAAGGCAGCTGCACGTGCTC CTTCCCAACCAGTGAGCTGGAGAAGGTGCTCCCCCAGACCATTCTGTACAAATACTATGAACGGAAAGCT GAGGAAGAAGTCGCTGCAGCCTATGCTGATGAGCTTGTCCGGTGCCCCTCCTGCAGCTTCCCTGCTCTGT TGGACAGCGATGTGAAGAGGTTCAGCTGCCCCAACCCACGCTGCCGAAAGGAGACCTGTAGGAAGTGTCA GGGACTCTGGAAAGAACACAACGGCCTCACCTGTGAAGAGCTGGCAGAAAAGGATGACATCAAGTACCGG ACATCCATTGAAGAAAAAATGACTGCTGCTCGGATTAGAAAATGTCACAAGTGTGGAACTGGCCTCATCA AGTCTGAAGGCTGCAACCGCATGTCTTGCCGCTGTGGTGCCCAGATGTGCTACCTCTGTCGAGTTTCTAT CAATGGCTATGACCATTTCTGCCAGCATCCTCGCTCTCCAGGAGCCCCTTGCCAAGAATGTTCCAGGTGC TCCCTGTGGACCGACCCCACTGAAGATGATGAAAAGCTGATTGAGGAAATCCAAAAGGAGGCCGAGGAGG AACAGAAGAGAAAGAATGGAGAGAACACCTTCAAACGCATCGGGCCCCCACTGGAGAAGCCAGCTGAGAA GGTGCAGCGTGTGGAAGCCCTGCCGCGGCCTGTCCCACAGAACCTGCATCCTCAGATGCCGCCCTATGCC TTTGTTCACCCACCCTTCCCCCTGCCACCTGTGCGGCCCGTGTTCAACAACTTCCCCATCAACATGGGTC CTGTGCCCGCTCCCTATGTCCCCCCTCTGCCCAACGTGCGTGTCAACTATGACTTTGGCCACATGCACGT GCCCCTGGAGCACAACCTGCCCATGCACTTTGGCCCCCAACCACGGCATCGCTTCTGACACCCAAAGCCC TGTCAGCCGTGCCGAGTCTGCAGGAGGGCCCAGTCTCATCTTCTGAGTAGGGGTGAAGGCCTCCATTCCC TCTCGAAAGTGGACGCGTGTCCTCCTGCTCTTACCTTTGCAAGGTCCATGCTCCTTCAGGTCTGATGCCC TCTGGGTGCTGATTGTCACTGGGCCAATTATAGGGCAGCTCCCTAGTCTGCCATCTTAGCAGCCAATCCA GTGGCCCTGACCATGAAGCAAGGCCTCTAATCGTTTGCCATACTTCCTCCCCAGCAGCCCAATGAAAGCC CAGGGGGAAATGGCCTACCATCCCTAAGCCAGGGCTCTCTCCTTGTTGCCCAAGGCCCACTTAGGTCCAA GAGCCTCCGAGAATCTGTGGCGCGAGTTTTGTTCTGTTGTTATCTAAAGAGGTTGCTCCTATTCATGTAG TTTGAGCTGAAGTTTTTTTTGTGGTCTACTCATGCGTGTCCCTATCCGTGGAAAGTCAGGTTGAAAGTAT TTCCCCAGGGCTGGCAACTGCTGAGAGCCAGAAGTCCTGACACTTGCCATCAAACAAAAGGGCCCTTTAG GAAGCTGTCATTTATTCCCAGGTGGGCCCTGTTCTGGGGTGAGCAGCTTCTTTCTCCTAAGGTGTTGGGG GTTCCCTGGCAGTGCAGATGACCTCATGGAGCTGCTACAGTCATCACAGCAACCCTGCTGCCTGCTCCCC GTCCCTCACTAGTTAGCAGTGGGTGCACACAGGAGAGCTCCCAGCTCCTGTGTGATGAGAGTCCAGGCCT GCACAGAGCCTTTGCCATGTTCCCAGTGCCTGTGTCCCCTCCTGTCCAGACCAGGCTTTTGTCCTCCAGC GTTTGCTGAGCAGGACCGGCAGCAGGTTTCTCAGTTGTACTGGCTGTCCTTGTCCTCCCCTTGCTGTGGG GGTGTCCCCTGTTCAGAGTGTACAGACTTAGAAGGGGGAAAGTCCCTGGGAAAGGGAAGGCTCCCTGAGC TGCCCATTATCACTGGAGTGTGGGTGGGGCACACAGTGGCTCAGGAGCCCTGCCATGGTCACCTGAACTG TGATGTTAGTCTGAATGACAGTGCCTTGGAAAGATGGGCCACATGCTGCATGACAGGATCCTCCAAGTAC CTTAACTGTTAGAGTATCCGTGTTCTCTGCACTTGTCCAGAAGCCCCACGCATGGGTGGGTGGCTGGGTG GGTACTCCAAGCACTGCCCCTCCACGGCCTCGTGAGCCGCTGGACTGGGCACCCAGTTCTAGGTTGACTC AGTACCCGTCTCACATCCTTCCACCAGGAGACCAGATGCAAACAACTGGCAGTTTCTGGGGTGAGGCCAG GGTCGTGTCAGTCTGTGGGTGCAGGGAGGCTGCTCCTGCCTGTCTCCATCCAGGAGTGACCCTCCCAACA CCTCTGTGCCAGCAGGGATCCCCAAGGCAGCTACCACCTTAACATCTAGGACCCGGACTGCTTCCTGGTG TGAGAAACTGCCTAGCCACTCCCAGTGTATTTGCCTTAAAACTGGAGAGAATCTAGTATTTGCACTTAGC AAAAGATTTCAAAGTTAAGAGAAAAGTGCATGATTTCACTTTGTACAAAAGAAAAAAAGAAAAAAAAATG AAGTAGATGGTTAAAAGTTTTTCTTTACCTTTTGGTAGTAAAAATAGAGGAACCAAGTCTGTTTAAACTA CTGTGTAAAAAGACTGTATCATATGTAACTTGACTTTTGTGTAAATAAATGTTTGTGAACGCTTAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6400486 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6400486 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7618 same embryo
	EMAGE:7617 same embryo
	EMAGE:7614 same embryo
	EMAGE:7616 same embryo
	EMAGE:7615 same embryo
	EurExpress:euxassay_004683 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source