EMAGE mouse gene expression spatial database

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EMAGE:7614

Phf12 PHD finger protein 12 ( MGI:1924057)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004699. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004699_01	euxassay_004699_02	euxassay_004699_03	euxassay_004699_04

euxassay_004699_05	euxassay_004699_06	euxassay_004699_07	euxassay_004699_08	euxassay_004699_09

euxassay_004699_10	euxassay_004699_11	euxassay_004699_12	euxassay_004699_13	euxassay_004699_14

euxassay_004699_15	euxassay_004699_16	euxassay_004699_17	euxassay_004699_18	euxassay_004699_19

euxassay_004699_20	euxassay_004699_21	euxassay_004699_22	euxassay_004699_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7614_wholemount_strong.wlz

7614_wholemount_moderate.wlz

7614_wholemount_weak.wlz

7614_wholemount_possible.wlz

7614_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7614_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8669
Entity Detected:	Phf12, PHD finger protein 12 ( MGI:1924057)
Sequence:	sense strand is shown >T8669 AGGGGACCGGGGGTCAGAGGTGCCCCCTTCCCCTCCTCCCCCCTCCCCCCGGGGCTGGGGGGGCCGGAGC AGAGAGCGCCCAGCCCGAGAGGTGGATGAATGTGGGAGAAAATGGAGACCAAGACGATCGTGTACGACTT GGACACGTCGGGGGGGCTGATGGAGCAAATCCAAGCTCTGCTGGCTCCCCCCAAGACGGACGAGGCAGAA AAGCGGAGTCGGAAGCCCGAGAAGGAGTCCCGGAGAAGCGGCAGGGCCACCAACCACGACAGCTGCGATA GCTGCAAGGAAGGTGGGGATCTCTTGTGCTGCGATCACTGCCCTGCCGCCTTCCACCTCCAGTGCTGTAA CCCTCCACTGAGTGAAGAGATGTTGCCTCCTGGGGAGTGGATGTGTCACCGGTGTACTGTCCGCCGAAAG AAACGAGAGCAGAAAAAGGAGCTTGGCCATGTCAATGGACTGGTGGACAAGTCTAGCAAACGGACTACAT CCCCCAGCAGTGACACTGACTTGTTGGACAGACCAGCTAGCAAAACTGAACTCAAGGCCATTGCCCATGC CCGGATCCTGGAAAGGAGAGCCAGCCGGCCTGGCACCCCCACATCCAACGCCAGCACAGAGACCCCCACC TCTGAGCATAATGACGTCGATGAGGACATCGTTGACGTGGACGAGGAGCCAGTCGCAGCAGAGCCGGACT ATGTGCAGCCCCAGCTGCGGCGGCCTTTTGAGCTGCTGATTGCTGCTGCCATGGAGCGGAACCCTACCCA GTTTCAGTTGCCCAACGAACTGACCTGTACCACTGCACTACCAGGTTCTAGCAAACGGAGAAGAAAGGAA GAAACCACAGGGAAGAATGTAAAAAGGACACAGCATGAGTTAGACCATAATGGTCTTGTTCCCTTGCCGG TCAAAGTCTGCTTCACATGTAACAGGAGTTGTCGAGTGGCCCCCCTCATCCAGTGTGACTATTGCCCGCT TCTGTTTCACATGGACTGCCTCGAGCCCCCGCTCACTGCCATGCCCCTGGGCAGATGGATGTGTCCGAAT CACATCGAACATGTGGTGCTGAACCAGAAGAACCTGACTCTGAGCAATCGGTGCCAGGTGTTTGATCGGT TCCAGGACACCATTTCACAACATGTTGTCAAAGTAGATTTCCTGAACCGAATCCACAAGAAGCACCCTCC TAACCGCCGTGTGCTCCAGTCTGTCAAAAGAAGAAGCTTGAAGGTTCCTGATGCTATAAAATCTCAGTAC CAGTTTCCACCCCCGCTTATTGCACCCGCGGCCATTCGGGACGGGGAGCTGATCTGCAGTGGGGTCCCTG AGGAATCACAAACGCACCTTTTGAACTCTGAGCACTTAGCCACTCAGGCAGAGCAGCAGGAGTGGCTCTG TAGTGTTGTTGCGCTCCAGTGCAGCATATTGAAACATTTATCTGCTAAGCAGATGCCTTCGCCTTGGGAC TCTGAACAGACAGAGAAGGCTGATATTAAGCCTGTTATTGTGACTGACAGCTCAATCACCACCTCCCTGC AAACCGCTGACAAGGCACCTCTTCCTTCCCACTACCCTTTGTCCTGCCCCTCAGCAGTGAGCACCCAAAA TTCCCTGGGCTGCTCTCCACCCCACCAACCCCCAACCCTAGAAGACATCAGCTGCAGTTCTTGTGTGGAA AAGTCCAAGAAAGCCCCTTGTGGGACTGCCAATGGGCCGGTGAACACAGAGATAAAAGCCAATGGCCCAC ACCTCTACAGCAGCCCCACTGATTCCACGGATCCCCGGCGACTTCCTGGCGCCAACACCCCTTTACCAGG CCTCACACACCGGCAAGGCTGGCCCCGGCCCCTCACGCCACCATCGGCTGGGGGACTTCAGAACCACGTC GGCATCATTGTGAAGACAGAAAATGCCACTGGCCCCAGCTCTTGTCCCCAGAGGAGTTTGGTACCCGTCC CAAGCCTGCCCCCTTCCATTCCCAGCTCTTGTGCCAGCATCGAGAACACCAGCACTTTGCATAGAAAGAC TGTCCAGTCACAGATAGGACCTTCGTCGACAGAATCGAGGCCTCTGGGCTCACCCCCAAATGCCACCCGG GTGCTCACTCCCCCCCAGGCTGCAGGAGACAGTATCTTGGCCACAGGTGCCAACCAACGATTCTGCTCAC CAGCGCCATCATCAGATGGCAAGGTCAGCCCCGGCACGTTATCCATAGGAAGCGCTTTAACCGTACCCTC TTTCCCAGCCAACTCTACTGCCATGGTGGACCTCACCAACTCACTTCGAGCATTTATGGATGTCAACGGA GAAATCGAGATAAATATGTTGGATGAGAAGCTGATCAAGTTTCTGGCCTTGCAGAGAGTACATCAGCTTT TCCCTTCCCGGGTCCAAGCTTCACCGGGCAATGTTGGGACACATCCGCTGGCTTCTGGAGGGCACCACCC AGAAGTGCAAAGAAAGGAGGTACAGGCCCGAGCTGTGTTCTGCCCCCTCTTAGGGTTGGGAGGAGCTGTG AACATGTGCTATCGAACCCTCTACATCGGGACAGGAGCTGACATGGATGTGTGCCTTACAAACTATGGTC ACTGTAACTACGTGTCCGGGAAACATGCCTGCATATTCTACGATGAGAATACCAAACATTATGAGCTGTT AAACTACAGTGAGCATGGGACAACGGTGGACAATGTGCTGTATTCATGTGACTTCTCTGAGAAGACCCCG CCAACCCCCCCAAGCAGTATTGTTGCCAAAGTACAGAGTGTCATCAGGCGCCGCCGACACCAGAAACAGG ATGAAGAGCCAAGTGAGGAGGCAGCCATGATGAGTTCCCAGGCCCAGGGGCCACAGCGGAGACCCTGCAA TTGCAAAGCCAGCAGCTCAAGCTTGATTGGGGGCAGTGGGGCCGGCTGGGAGGGCACAGCCTTACTCCAC CATGGCAGCTACATCAAGCTGGGCTGCCTGCAGTTTGTCTTCAGCATCACTGAGTTTGCGACCAAACAGC CCAAAGGCGATGCCAGCCTGCTGCAGGATGGGGTCTTGGCTGAGAAACTCTCTCTCAAGCCCCATCAGGG CCCTGTGCTGCGCTCCAACTCCGTTCCCTAGGCCATTGGCCTGGACGCCCACCCAAGACTCCTGCAATGC AAAAATGTACACGAACCAAGCCTGGGTGTTTTCTATACCAGAAACCCTCAACTACAATCTTTGCATGAAA TGAAGAAAACCTTTTGACTGTTTTTTAAGACTTTTTTTCTTTTCTCAAGTTCTAGGGGGCATTTGCACAT ATATTTGTACTCAACATTTCATGGGAAAGCGGCAGATCCGCGCTGAGGAGCAGCGAGGGCAGGGACAGGA GGCCCTGGTCTGGACACTTCCTCCAGCACAATCCCTTCCCCCGCCTCCTGCTCCTCCCCCTCGACCGCCT GCCCACTGTTGTAAAATAATCAGAAACTTGTTCTATTTTGTGGCAGTGACAATAGTTTTATATTAAAAGA AAAAAATACAGTTTTCATACAGCAAAATCTATACAATATCATTGTTTTATTTAATATAAAGATCGCTACC CACTCCTTTCCATGGTTCCCACCCTACAAGGACTTCCCCTCTCTGCAGCAGTTGCACTACAGGTAGCTAC TGTGTATATGGACAAATGAGAAATGAATCCTTTTTTCTGGCTGTCCATCTATTTTATTTCAAATAAGGAA AAGTGTATTTGGATTTTGTGTAAATACATCTAGTGATGGCATTTTTTCAATGTTTTTAAAAGCTGTGTAC AGTACATGTGGTAGAGTGTTTCTCAAATTGTCTATTGTAGCAAAGGCGTTTTTGTCGTAAACCTGTTCTG TGTCCTTTTTTGTTCTTACCACTTCTCTTCCTCCTCACCCCAGATACTTCCTCTTCCCCACAACCAATGT ACATAGTAATTGTAATGTTTTAGACTTGACAGAAACTTTCCTGTATTCTGTATATAAAAACCAAAAATAC TTCAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6400617 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6400617 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7613 same embryo
	EMAGE:7618 same embryo
	EMAGE:7617 same embryo
	EMAGE:7616 same embryo
	EMAGE:7615 same embryo
	EurExpress:euxassay_004699 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source