EMAGE mouse gene expression spatial database

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EMAGE:7616

Pkn2 protein kinase N2 ( MGI:109211)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004700. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004700_01	euxassay_004700_02	euxassay_004700_03	euxassay_004700_04

euxassay_004700_05	euxassay_004700_06	euxassay_004700_07	euxassay_004700_08	euxassay_004700_09

euxassay_004700_10	euxassay_004700_11	euxassay_004700_12	euxassay_004700_13	euxassay_004700_14

euxassay_004700_15	euxassay_004700_16	euxassay_004700_17	euxassay_004700_18	euxassay_004700_19

euxassay_004700_20	euxassay_004700_21	euxassay_004700_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7616_wholemount_strong.wlz

7616_wholemount_moderate.wlz

7616_wholemount_weak.wlz

7616_wholemount_possible.wlz

7616_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7616_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8671
Entity Detected:	Pkn2, protein kinase N2 ( MGI:109211)
Sequence:	sense strand is shown >T8671 CCGCCGCGGCCGCTGCGCCCCGCACCGAGAGCTCCGAGTCGGCTCCCGGCGTCGCCCTGAGGGAGCGGCT GAGGGAACAGAGTCCGGCGGGCGGCGGCGGGCGGCGAGGGGAGCCGCGCCGCGAGGGCCCTCGCTCCCCG CCGCTCTCCATGAGCCGCGCCTGCAAGCCGGGCCGCGCCTCCATGACTCCCTAGCTGCCGCTTTCTCTCC GGTCCTCGCCCCCCGCCCGGCTCTCCCGTCCCGCCTTCTCCCTCGGCCGCGCCGCTGGGCGCCTTCAGGT GCGGAGGCCCGAGCGGAGCGCCATGGCGTCCAACCCCGACCGGGGGGAGATCCTCCTCACCGAGCTGCAG GGAGATTCCCGGACTCTTCCATTTTCTGAGAATGTGAGTGCTGTTCAGAAATTGGACGACATCAAGGATC GAATAAAGAGAGAAATAAGGAAAGAACTGAAAATCAAAGAAGGAGCTGAAAACCTGAGGAAAGTTACAAC AGATAAGAAAAATTTGGCTTATGTAGACAACATTTTGAAAAAATCAAATAAAAAATTAGAAGAATTACAT CACAAGCTACAAGAATTAAATGCACACATCGTTGTATCGGATCCAGAGGACTCCACAGATTGTCCGAGGA CTCCAGATACTCCAAATAGTGACTCTCGTAGTTCTACTAGCAACAATAGACTGATGGCCTTACAGAAACA ACTGGATATAGAACTTAAAGTAAAGCAGGGTGCAGAGAACATGATACAGATGTACTCAAATGGCTCTTCA AAGGATCGGAAACTCCATGGTACAGCGCAGCAACTGCTACAGGATAGCAAGACAAAAATAGAAGTCATCC GGATGCAGATTCTTCAGGCCGTCCAGACCAATGAGCTGGCTTTTGATAATGCAAAACCTGTGATAAGCCC TCTTGAGCTTCGGATGGAAGAATTAAGACATCATTTTAAGATAGAATTTGCAGTAGCCGAAGGTGCAAAG AATGTCATGAAGTTACTTGGCTCAGGAAAAGTAACAGACAGAAAAGCGCTTTCAGAAGCACAAGCGAGAT TTAATGAATCAAGTCAGAAGTTGGACCTTTTAAAGTATTCACTAGAGCAAAGGTTAAATGAGCTCCCCAG GAATCACCCCAAAAGCAGTGTCGTCATTGAGGAGCTTTCACTGGTTGCGTCACCAACCCTGAGTCCACGG CAGAGCATGCTGTCCACGCAGAACCAGTATAGCACACTGTCCAAGCCAGCGGCGCTAACCGGTACTTTGG AAGTTCGTCTTATGGGGTGCCAAGATATCCTAGAGAATGTACCTGGACGGTCAAAAGCAACGTCGGTAGC ACTACCTGGGTGGAGTCCAAGTGACAACAGATCGTCTTTTATGAGCAGAACCAGCAAGAGTAAAAGTGGG AGTAGTCGCAACCTCCTGAAAACGGATGACTTGTCCAATGATGTTTGTGCTGTTTTGAAGCTAGATAATA CTGTGGTTGGTCAAACTAGCTGGAAACCCATTTCAAATCAGTCGTGGGACCAGAAGTTTACACTGGAACT AGACAGGTCACGTGAACTGGAAATTTCAGTTTATTGGCGTGATTGGCGATCTCTGTGTGCTGTAAAATTT CTGAGGTTAGAAGATTTTTTAGACAACCAGCGGCATGGCATGTGTCTCTATTTGGAACCACAGGGTACTT TGTTTGCAGAGGTTACTTTTTTTAATCCAGTTATTGAAAGACGACCAAAACTCCAAAGACAAAAGAAAAT TTTTTCAAAACAACAAGGCAAAACATTTCTCAGAGCTCCTCAGATGAATATTAATATTGCCACTTGGGGA AGACTAGTGAGACGAGCTATTCCCACAGTAAATCATTCTGGCACATTCAGCCCTCAGACTCCTGTGCCTG CAACAGTGCCAGTCGTTGACGCACGCATTCCTGATCTAGCACCTCCGGCCAGTGACTCTACAGTAACCAA ATTGGACTTTGATCTTGAACCTGAACCTCCTCCAGCCCCACCTCGTGCTTCTTCCCTTGGAGAGACAGAT GAATCTTCTGAATTGAGAGTTTTGGATATACCTGGACAGGGTTCAGAAACTGTTTTCAATATTGAGAATG ACAGAAATAACCTGCGTCCAAAATCCAAATCTGAATATGAGCTGAGCATTCCTGACTCAGGTCGAAGCTG CTGGGGTGTTGGAGAGCTTGATGACAAGAGAGCTCAGCAGAGGTTTCAGTTCAGTCTACAGGACTTCAGG TGCTGTGCTGTCTTGGGGAGAGGACACTTTGGAAAGGTGCTGTTGGCGGAATATAAACACACAAATGAAA TGTTTGCAATAAAAGCCTTAAAGAAAGGAGACATTGTGGCACGAGATGAAGTAGACAGCCTGATGTGTGA AAAAAGAATTTTTGAGACTGTGAATAGTGTCAGGCACCCGTTTCTAGTGAACCTTTTTGCATGTTTCCAA ACAAAAGAACATGTTTGCTTCGTGATGGAGTATGCTGCAGGTGGTGACCTGATGATGCACATCCATACTG ATGTCTTCTCTGAACCCAGAGCTGTATTTTATGCAGCATGTGTAGTTCTTGGGTTGCAATACTTACATGA ACACAAAATTGTTTATAGAGATTTGAAGTTGGATAACTTGTTGCTAGATACAGAAGGCTTTGTGAAAATT GCTGACTTTGGTCTTTGCAAAGAAGGAATGGGGTATGGAGATAGGACAAGCACATTTTGTGGTACTCCTG AGTTTCTTGCCCCAGAAGTCCTAACAGAAACATCGTACACAAGGGCTGTAGATTGGTGGGGCCTTGGTGT ACTTATATATGAGATGCTCGTCGGCGAGTCTCCCTTTCCTGGTGATGATGAAGAAGAGGTTTTTGACAGT ATTGTAAATGACGAAGTAAGGTATCCAAGGTTCTTATCTACAGAAGCCATTTCCATAATGAGAAGGCTGC TAAGGAGAAATCCTGAGCGGCGCCTGGGAGCTGGTGAGAAGGATGCAGAGGATGTCAAGAAGCACCCGTT TTTCCGGCTAACCGACTGGAGTGCTCTAATGGACAAAAAAGTAAAGCCACCATTTGTACCTACCATTAGA GGACGGGAAGATGTTAGTAACTTTGATGATGAATTTACCTCAGAAGCACCTATTCTGACTCCACCTCGAG AACCAAGGATACTCTTGGAAGAGGAGCAGGAGATGTTCCACGATTTTGACTACGTTGCTGATTGGTGTTA AGTTTCTAGACCCTGAGACCAAGCAGACTGACCAACAAGAAGACCTCTTGAAAACAGCAGCCCTGCATTT CCTTTCCGTGCCACCAGGAGCTTCTGAGATTATATAAAGCACATGAAGATGTGGTTACAGTACTGTTTTA TACCTTAATGTTTTCAACGTAAATCTGAACACTGGAAGCAGTTTCATGGAGTTTAGGCTGAGTGAACATT GGCCATGGAAATCCATTACAAATAAATACTTTTACATCAATACTCTTATTTTTAAAAAGAAGAAGAACCA CTTTTTTATAGTCCCCATGTTTGCCCTAGGCCTGCCTTAAGTGGAAGGAAGATTAATCACTTAACTATAT TTTACAAAAGAAAAAATAGGGCTTTGGATGCTATTACTCGTAATATAAAAAGTGTGATTCTATTTCACTA AGGCATTATTTTTTTAAGAAATCACTATAATATGAAAAAATGTGGCAGTTTGTACACCTTTGGTGGCTTG ATTTAAAGAAAAGAATGAGAATGTATTGATGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6400440 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6400440 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7613 same embryo
	EMAGE:7618 same embryo
	EMAGE:7617 same embryo
	EMAGE:7614 same embryo
	EMAGE:7615 same embryo
	EurExpress:euxassay_004700 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source