EMAGE mouse gene expression spatial database

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EMAGE:7633

Nup85 nucleoporin 85 ( MGI:3046173)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004729. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004729_01	euxassay_004729_02	euxassay_004729_03	euxassay_004729_04

euxassay_004729_05	euxassay_004729_06	euxassay_004729_07	euxassay_004729_08	euxassay_004729_09

euxassay_004729_10	euxassay_004729_11	euxassay_004729_12	euxassay_004729_13	euxassay_004729_14

euxassay_004729_15	euxassay_004729_16	euxassay_004729_17	euxassay_004729_18	euxassay_004729_19

euxassay_004729_20	euxassay_004729_21	euxassay_004729_22	euxassay_004729_23	euxassay_004729_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7633_wholemount_strong.wlz

7633_wholemount_moderate.wlz

7633_wholemount_weak.wlz

7633_wholemount_possible.wlz

7633_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7633_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8715
Entity Detected:	Nup85, nucleoporin 85 ( MGI:3046173)
Sequence:	sense strand is shown >T8715 GAGGCCTGAGCGCAGAGAAGCGGCCGCGCGGCAGGGTCCCAGCCGCGGGGGGCACGGCGCTATGGAGGAG CTCGACTGCGAGCCCGCAGTAACTTGGATTCCAGGTGTGAATTCCAAGAAGAAGCAAATGTGTTTTGACT GGGGCCCCGGCGAGATGCTTCTGTGTGAAACTTCCTTCAACCAAACAGGCAAATCAGAGAAGGTGCCGAG CTGCCCTTTTATCTACATCATACGGAAAGATGTGGATGTTTACTCTCAAATTTTGAGAAAACTCTTCAAT GAATCCCATGGAATCTTTGTTGGCCTGCAAAAAATTGAGGAAGAACTGTCGGGGAAGTCCAGGAAAGCTC AATTGGTTCGAGTGAGTAAAAATTACCGTTCAGTCATACGGGCCTGTATGGAAGAAATGCATCAGGTTGC AATTGCTGCTAAAGATCCAGCCAGTGGCCGGCAGTTCAGCAGCCAGGTCTCCATTTTGTCAGCCATGGAG CTCATTTGGAACCTGTGTGAGATTCTCTTTATTGAAGTAGCACCAGCTGGCCCTCTCCTCCTTCACCTTC TTGACTGGGTCCGACTGCACGTGTGCGAGGTGGACAGTTTGTCGGCAGATGTCCTGGGCGGTGACAACCC AAGCAAGCATGAAAACTTCTGGGACCTGGTGACTGTTCTGGTGCTTCAGGGCCGGCTCGATGAGGCACGG CAGATGCTAGCCAAAGAAGCTGATGCCAACCCCTCTTGTGCAGGCATGTGCCGCGTCCTTGGGGACCTGA TGAGGACAATGCCCATTCTCAGCCCTGGCAATACTCAGACACTGACAGAGTTGGAGCTGAAGTGGCAGCA CTGGCGTGAGGAGTGTGAAAGACACTTACAAGACAACACATTTGCAGCCAACCCCCGTCTGGAGTCTCTC TGCAAGATCATGCTGGGAGATGAGGCCGCCTTGTTGGAGCAGAAGGAGCTTCTGAGCAACTGGTACCATT TCTTAGTGACGAGGCTGCTGTACTCTAACCCCACAGTGAAGCCCATTGACCTGCACTTCTATGCCCAGTC CAGCCTAGACATGTTTCTTGGAGGTGAGAGCAGTCCAGAACCACTGGACAACATCTTGATGGCGGCCTTT GAGTTCGACATTCACCAGGTGATCAAAGAGTGCAGCATCGCCCTGAGCAACTGGTGGTTCGTAGCTCACC TCACAGACCTTTTGGATCACTGCAGACTCCTCCAGTCACACAATCTCTATTTTGGTTCTAACATGAGAGA ATTCCTCCTGCTGGAGTACGCCTCAGGACTGTTTGCTCACCACAGCCTGTGGCAGCTGGGGGTGGACTAC TTTGATTACTGCCCGGAGCTAGGCCGAGTTTCCTTGGAGCTGCACATTGAGCGGATTCCTCTCAACACAG AGCAGAAAGCCTTGAAGGTGCTGAGGATTTGTGAGCAGCGGCAGATGACTGAGCAAGTTAAAAGCATCTG TAAGATCTTGGCCATGAAGGCTGTTCGTAATAACCGCTTGGGCTCAGCACTCTCCTGGAGCATCCGTGCC AAAGATGCTGCCTTTGCCACACTCGTATCTGACAGATTCCTCCGGGATTACTGTGAGAGAGGCTGCTTTT CTGACTTGGATCTCATTGACAATCTGGGGTCAGCCATGATGCTCAGTGATCGACTGACGTTTCTGGGAAA GTACCGGGAGTTCCACAGACTGTACGGGGAGAAGCGCTTTGGTGACGCTGCTTCTCTTCTGCTGTCCCTC ATGACCTCTCAGATTGCACCTCGTTCTTTCTGGATGACTCTGCTCACAGATGCCCTTCCTCTTTTGGAAC AGAAACAGGTGATTTTTTCAGCAGAGCAGACATATGAGCTGATGCGGTGCCTGGAAGACTTGGCCTCAGG GAGGCCAGAGTGTGGTGAACCTGATGCCCAGCGACTGCAGGATGACGACATAGAGACCACCAAGGTGGAG ATGCTGAGACTGGCTCTTGCCCGGAATCTTGCTCGGGCAATTATAAGGGAAGGCTCACTGGAGGGTTCCT GAGAGAAGATGAGGACAGTTGCAATATAGTAACTTTGTATGGCAATGTAAATAGATTTTAAAGAATAAAT TGTTTTGCAAATATAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6403446 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6403446 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7634 same embryo
	EMAGE:7631 same embryo
	EMAGE:7632 same embryo
	EMAGE:7636 same embryo
	EMAGE:7635 same embryo
	EurExpress:euxassay_004729 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source