EMAGE mouse gene expression spatial database

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EMAGE:7635

Tpm4 tropomyosin 4 ( MGI:2449202)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004736. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004736_01	euxassay_004736_02	euxassay_004736_03	euxassay_004736_04

euxassay_004736_05	euxassay_004736_06	euxassay_004736_07	euxassay_004736_08	euxassay_004736_09

euxassay_004736_10	euxassay_004736_11	euxassay_004736_12	euxassay_004736_13	euxassay_004736_14

euxassay_004736_15	euxassay_004736_16	euxassay_004736_17	euxassay_004736_18	euxassay_004736_19

euxassay_004736_20	euxassay_004736_21	euxassay_004736_22	euxassay_004736_23	euxassay_004736_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7635_wholemount_strong.wlz

7635_wholemount_moderate.wlz

7635_wholemount_weak.wlz

7635_wholemount_possible.wlz

7635_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7635_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8716
Entity Detected:	Tpm4, tropomyosin 4 ( MGI:2449202)
Sequence:	sense strand is shown >T8716 GTCGCCGGAGCCCAGCAGAACGATTGAGCTATGGCCGGCCTCAACTCACTGGAGGCAGTGAAGCGCAAGA TCCAGGCCCTGCAGCAGCAGGCAGACGACGCAGAGGATCGCGCGCAAGGCCTGCAGCGCGAACTGGATGG CGAGCGCGAGCGGCGCGAGAAAGCTGAAGGAGATGCAGCCGCTCTCAACCGCCGCATCCAACTGCTGGAG GAGGAACTGGACCGGGCTCAGGAGCAGCTGGCCACAGCCCTGCAGAATCTGGAAGAGGCAGAGAAGGCTG CTGATGAGAGTGAGAGAGGCATGAAGGTAATAGAGAACCGAGCCATGAAAGATGAGGAAAAGATGGAGAT CCTGGAGATGCAGCTCAAAGAAGCCAAGCACATCACTGACGAAGCCGACCGCAAGTATGAGGAGGTTGCT CGTAAGTTGGTCATCCTGGAGGGTGAGCTGAAGAGAGCAGAGGAGAGGGCGGAGGTATCTGAACTAAAGT GTGGTGACCTGGAAGAAGAGCTCAAGAATGTAACTAACAATCTGAAATCACTGGAGGCTGCTTCTGAAAA GTATTCTGAAAAGGAGGATAAATATGAAGAAGAAATCAAGCTTCTGTCTGACAAGCTGAAGGAGGCTGAG ACCCGAGCTGAGTTTGCAGAAAGGACAGTTTCTAAACTGGAGAAGACAATCGATGACCTGGAAGAAAAAC TTGCCCAGGCCAAAGAAGAGAATGTGGGCTTACATCAGACACTGGACCAGACACTAAACGAACTTAACTG TATATAAACTAAACCAGAAGAGTACTGTTTTAACACCAACTCCACTCCAGAGAGTGTGCCCTGTCTTCCT CTTTTGTAAGAAGTTCCGCTTATTACCAGGTTTCCACCTTGCAGGAAATGCCAAGCAGATAATGAATTCA TGACCAAATATTTTGTGTTAGACAAGCTTTGAGCACCAGTTAAGTTTAATTCTTTCCTTTTCCATTCAAA TGGCACCAGCTTCTCTGATTATTCTTATTCTTAAATTGCATTTATTCCTAAAATAGGCAGGGAATTTCAC ACTGAGTATAGTTGCTTAAGTCTGAGGGCATTTGGTTCTTGGGAATTAGTCAGCCCCACACTTTGAAGCA CAGGCTCAAGTATCTAATCTTAGCTGGTTGCTGTTGGGGAAGTTTTGTGAGGCTAGGGGTTGTCACAAGA AAATATGTGGAAATTTTCCACCCAGAGCAAAAATTAAGTCTCCTATGGGAAGTTATAAAGCACAACTAGA TTAGCCTGATCATACTGAGTCTGCTCCACACGGCCCTGTGCAGAACAGATTGTCTTTAAGCATTCCAAGC GAGAATGCTGGGGTGATCATTGTTGCCCATGTGCCTTCTGACTGAGCTTTAATGCTGTTCCATGTTACAT CTTTCCTTCACTCCAGAATTGTTCTCTGAGGAGCAAGCGTCTTATTCCAGGACTTTTCTGCTGTGGTCTA ATGATATACCCAGCAGGGAATATGGCAGAGTCCAACCCTTTTCAGCCAGGGCTATACGGCGAAACTCTGT CTCAAATAATAAAAAGGCTTAGTTGCCTTCAATACATTGAGCATTGTACTAATACCAAGTGTTGGGCAAC AAAAGGTCTCTCCAGCAGCAAAGTAGTGATAGAAAAAGAAATTGGCTTAACTTCATCTCCATTGTTCAGG GAACAACAAATGGAAGCTTGACTTTGCCAAGACTTGAATTCCTTCGGGAATGACACTAATAAAAGACCAA GACCTCCTGATTGAACTGGATGTGGAGTGCTCTGTAGGCTGCACATTTTTTGGCTTTGTAGTTTTCCTTT CTCAGTTAACATCTCACACTGAAACATTCCACATTCCCCAGCAGTGCGGGGCCAACTCAAGTTTACAGTC CTGACTGAAAGTCACCCTGCTTCTAGCTTATCTGAACCCTGCCCCTCACTCCTATTTATTAAGCATCACA CTACCAGGGAGATGAACTTTGAGCAAACTGCAATTGATAAAATCTACTCTGTTCTTTATGTTCTTGCTAT TGTATCATACGTAATAGTATCACTTTTTCTACATTTTGGTCAAATAAATTTTTGCATAAATAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6403300 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6403300 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7634 same embryo
	EMAGE:7631 same embryo
	EMAGE:7632 same embryo
	EMAGE:7633 same embryo
	EMAGE:7636 same embryo
	EurExpress:euxassay_004736 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source