EMAGE mouse gene expression spatial database

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EMAGE:7636

Rrm2 ribonucleotide reductase M2 ( MGI:98181)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004733. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004733_01	euxassay_004733_02	euxassay_004733_03	euxassay_004733_04

euxassay_004733_05	euxassay_004733_06	euxassay_004733_07	euxassay_004733_08	euxassay_004733_09

euxassay_004733_10	euxassay_004733_11	euxassay_004733_12	euxassay_004733_13	euxassay_004733_14

euxassay_004733_15	euxassay_004733_16	euxassay_004733_17	euxassay_004733_18	euxassay_004733_19

euxassay_004733_20	euxassay_004733_21	euxassay_004733_22	euxassay_004733_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7636_wholemount_strong.wlz

7636_wholemount_moderate.wlz

7636_wholemount_weak.wlz

7636_wholemount_possible.wlz

7636_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7636_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand

strong

regional

strong expression: see section 01 02 20 21 22 23

foot

strong

regional

strong expression: see section 01 02 03 04 05 06 07 17 18 19 20 21 22 23

leg

strong

regional

strong expression: see section 01 02 03 20 21 22

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vibrissa

strong

regional

strong expression: see section 05 06 07 08 19 20 21 22

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 13 14

telencephalon mantle layer

strong

regional

strong expression: see section 09 10 15 16 17

telencephalon ventricular layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hindbrain

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain ventricular layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17

spinal cord

strong

regional

strong expression: see section 11

retina

strong

regional

strong expression: see section 01 02 03 04 05 21 22 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 17 18 19

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

metanephros

strong

regional

strong expression: see section 05 06 07 08

lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

tail

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8713
Entity Detected:	Rrm2, ribonucleotide reductase M2 ( MGI:98181)
Sequence:	sense strand is shown >T8713 TTTAAAGGGCGCGGGCGCTGGCAGTCGGCGGTGCACCGGATTCCAGCTGTTTTCGCCTGCTCCTCGCCGT CTCCGCCGCTGCCCTCGTTCGCCATGCTCTCCGTCCGCACCCCGCTCGCCACCATCGCTGACCAGCAGCA GCTGCAGTTGTCGCCGCTGAAGCGACTCACCCTGGCTGACAAGGAGAACACGCCCCCGACTCTCAGCAGC ACCCGCGTCCTGGCCAGCAAAGCTGCGAGGAGAATCTTCCAGGACTCCGCCGAGCTGGAAAGTAAAGCGC CTACTAACCCCAGCGTTGAGGATGAGCCGTTACTGAGAGAAAACCCCCGCCGCTTCGTTGTCTTTCCCAT CGAGTACCATGATATCTGGCAGATGTACAAGAAAGCCGAGGCCTCCTTTTGGACTGCCGAGGAGGTGGAC CTTTCCAAGGATATTCAGCACTGGGAAGCTCTGAAACCCGATGAGAGACATTTTATATCTCACGTTCTGG CTTTCTTTGCAGCGAGTGATGGCATAGTCAATGAGAACTTGGTGGAGCGATTTAGCCAAGAAGTTCAAGT TACAGAGGCCCGCTGTTTCTATGGCTTCCAAATTGCCATGGAAAACATACACTCTGAAATGTACAGTCTC CTTATTGACACTTACATTAAAGATCCCAAGGAAAGAGAATATCTCTTCAATGCTATTGAAACAATGCCTT GTGTGAAGAAGAAGGCTGACTGGGCCTTGCGCTGGATTGGGGACAAAGAGGCTACGTATGGAGAACGCGT TGTGGCCTTTGCCGCCGTAGAAGGAATCTTCTTTTCCGGTTCTTTTGCATCGATATTCTGGCTCAAGAAA CGGGGGCTGATGCCGGGCCTTACATTTTCCAATGAGCTTATTAGCAGAGACGAGGGTTTACACTGTGACT TTGCCTGCCTGATGTTCAAGCACCTGGTACACAAGCCAGCAGAGCAGAGGGTCCGAGAGATAATCACCAA CGCCGTTAGGATAGAGCAGGAGTTCCTCACGGAGGCCTTGCCCGTGAAGCTCATCGGGATGAACTGCACT TTGATGAAGCAGTACATTGAGTTTGTGGCCGACAGGCTTATGCTGGAGCTGGGTTTTAACAAGATTTTCA GAGTAGAAAATCCGTTTGACTTCATGGAAAATATCTCACTAGAAGGAAAGACAAACTTCTTTGAGAAGCG AGTAGGCGAGTATCAGAGGATGGGAGTCATGTCGAATTCGACAGAGAACTCTTTTACCTTGGATGCTGAC TTCTAAGTAACTGATCGTGTGTTCTTCGCTGATTTTTGTCCCCTTGCCATTAAAAGAAACCAGCAAAAAC AACCAACTGGCTACACCATGAATTGTCATTAAATTTGCTAAACAGGTGTCTAAAAAGCTGTGTAGCTACC TCAGTCCTGTTTGCCAGGCTGGTCACTAGAAGAAAGTATACTTCAAACAATGGGTACTTGGATCCTTAGG GAGATCCTGTCCTTGGCTTTTACAAGTAGTGTGGTCACCTTTGACCTCATCAAAGTACTAACAGCACTGG GCCAGGTTTTAGGAGCAGTGACCATCAAGCAAGCAGGTTTAAACATTTAGATGCTGTTTAGGGCTGTTTA AAGATGTCGGACTGCTTCCTGCAGGCATGCAGGGTCTACTTAACAAGTTTGTAAATAAAATTGGCACTTT GCACACACACACACATAGTGCTGTCAGGCGATTAAACTATACATTTTATGAGGTAGTACCTCTATGCTTT TTTTTTTTTTTTTTAATGCTCAGTATTATCTTGAAGTTTGCAAATGCTATGATGGTACAGTAAATTCTGA CATTTGCCCTAATAGTGTCACTTTTTTTTTTTCTTCGAGACAGAGTTTCTCTGTATAGCCCTGGCTGTAC GGAATTCACAAGTGAGTTTGAGCCCAGTGGTGGGTACACCCGTGGGACTCTTACAAACCAAAACAGGAAA AGCAAGTGTTCCCTGAGGTAGTTTACTGTGATCTAGCTTCCTCATGAACTGACATAACCCTGATCAGTTT CCTTGATTATTGTATAGATGTTTTTGTAATATGAAAAGCCTTTGTACCTTTTAAATTATTGTTACTTAAA ATTAATAAACTCTTGAATTAACAGTCTTGAACTTTCATGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6402725 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6402725 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7634 same embryo
	EMAGE:7631 same embryo
	EMAGE:7632 same embryo
	EMAGE:7633 same embryo
	EMAGE:7635 same embryo
	EurExpress:euxassay_004733 same experiment
	MGI:4827817 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source