EMAGE mouse gene expression spatial database

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EMAGE:7667

Zfp180 zinc finger protein 180 ( MGI:1923701)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005294. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005294_01	euxassay_005294_02	euxassay_005294_03	euxassay_005294_04

euxassay_005294_05	euxassay_005294_06	euxassay_005294_07	euxassay_005294_08	euxassay_005294_09

euxassay_005294_10	euxassay_005294_11	euxassay_005294_12	euxassay_005294_13	euxassay_005294_14

euxassay_005294_15	euxassay_005294_16	euxassay_005294_17	euxassay_005294_18	euxassay_005294_19

euxassay_005294_20	euxassay_005294_21	euxassay_005294_22	euxassay_005294_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7667_wholemount_strong.wlz

7667_wholemount_moderate.wlz

7667_wholemount_weak.wlz

7667_wholemount_possible.wlz

7667_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7667_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8926
Entity Detected:	Zfp180, zinc finger protein 180 ( MGI:1923701)
Sequence:	sense strand is shown >T8926 GGCAGTGCAGCCATTTGTGTGAGTGTGTGTGTGTGTTTGTATGTGTTTGTGTGTGTGCGTGCGTGCGCGT GAGTGTGTGTGTGTGTATGTGTGTGAAGGCGTCTTCTATGGCTCAGCTTTGTGGCTGGGCTCAGTCTACA CGGAGCGACCTGGCGGATGCTGACTCGAAGTGGAAGCCAGACCCCCGCCACTCGAGTCATGCCGGGTGTG CTTCAGGATCGGGGCAGGAAGCGGTGAGCACAGTTTCGTTCTGACGCTGAGTCGTGAGTGGCTCAGACCC GCCCCGGTCCTCACCACGGGCTCACGGACGTCCAGCTTGGCACCACGCCACACCTGGAGGAGACCATGGA GCAACAAGATGATGAGTCCCTGGAGTCCCTGCAGGCCTGTGTGGAGAACTTTGTTCTACCCCAAGAGATG ATCATTGAAGTGGAGGGAGAAGATGCTGGGTCACTGGACATCATGCCTCAGGAAAGCACAGACTTCAAGA TTGTAACTGTGGACCTTACAGAGGAGGGACAGAGCATGTGGAACAATGCCCAGAGAACCCCAGAGAAAAC CGTGATCCTGGAAGGCCACAGGGACCTGTGGGACTTGCCAGCTGTACATGGAACAGAAGGAGCCACCGCA AAGCAGACAATCTTCAATGAAGAACCATCCCATGGATTGAAGCTAGAGGGGTCGGCAAGAGATGACCCTT GGCTGTCAACATGTGAAGAAGTCCGAGACTGTAAGGAGCAGTCGGGGAAACAACCGAACAAGCAAGAGGA GGTGGCCTTTACCCAGGGTAGCGCTGGCTCTCCTGATACTGTCTACAGGGGTCATGACCCTGACAGGAGT TGCCTGAGTACCAGCCGGCCTTCATTCCCCATGATACCCCTCAGAAACCATTTCCATAAACAGGCGTCAC ATGTTAAAAAGTTGCATGGTGATGTCGTTGCAAGCAGTCACCAAAAGATCAGTGATAGCTCAGATCTCCT TGAAGATGACACAGCTGGGGCAGTTCCTCAGAGCATCCGCCCCTCTCAGTTCGTAAGCAGCCCACAAGGA GAGGAAGCCTATGAACTTAATGGCCATGTTCAAGCTTCTGGCCGAGGTGCGCCCCGAAATCTAAATGAGA AAGTTCCTTTGGAAGGTAAAACCTTTGGCTTTAAAGGGCGCGGGCAAGTTTTAAACCATAGCGTGGTCCT CGGTGGGCAACAGGAGCTTCTTGTTAAAGAGGGCCAGGATAAATGTAAAACCGCCCCAAGTCCGTCTCTT CCTCAGAGCCTGAGAAACTACTCTGAGGAGAAGCGTTTTGAATGTAAGTACTGCGGCAAGTCCTTCAGCT GGAGCTCCCATCTCATTGCACACCAAAGAACCCACACAGGGGAAAAGCCCTACAAGTGCAACCTGTGTGG GAAGTTCTTCACCCGGAGCTCACATGTTGTTTCTCATCAGCGGATCCATACTGGAGAGAAACCGTACAGG TGCAATCTGTGTGGGAAGTCCTTTACCCAGAGATATGTTCTTGTGGTGCATCAGAGAACTCACACTGGCG AGAGGCCTTATGAGTGCACCCAGTGTGGGAAGTCGTTCCGCCAGAGCTACAAACTCATCGCACATCAAAG AACCCACACAGGGGAAAAGCCATACGAATGCACCCAGTGTGGGAAGTCGTTCATCCAGAGTTACAAGCTC ATCGCGCACCAGAAAATTCATTCTGGAGAAAAACCCTATGAGTGCAGTCACTGTGGCAAGTCCTTCAGCC AAAGCTATAAACTTGTTGCACATCAGAGGACTCACACCGGCGAGAAGCCCTTCGAGTGTAATCACTGTGG GAAGTCGTTCAGCTGGAGCTCGCAGCTGGTGTCGCATCAGAGAACCCACACAGGAGAGAAACCTTACGAG TGTAATGAGTGTGGGAAGTCATTCAACCGGAGCTCACACCTTGTCATGCACCAGAGAACTCACACTGGGG AGAAGCCGTATCAGTGCAAGCAGTGCGGGAAATCCTTCAGTCAGAGCTACGTCCTCGTCGTTCATCAGAG GACGCACACTGGGGAAAAGCCCTACGAGTGCGGCCAGGGTGGGAAGACGTTCAGGCAGAGCTCCTGCTTT ACTCAGCATCAGAGGACTCACACTGGAGAGAAGCCCTACGAGTGTAATCAGTGTGGGAAGACGTTCAGCC TAAGTGCCCGGCTTATCGTCCACCAGCGAACTCACACTGGGGAAAAGCCCTACAAGTGCGGCCAGTGTGG GAAAGCCTTTATTAGCAGTTCGAAGCGCAGTAGGCACCAGGCTACTCACAGTGAGGACGCCTGTAAGTCC TGACCAGTTGAGAGTCAGAGCTGATGTTGACTCCTGGACTGATCCAATCCTCTCAAGTTATGTGGGAAGG AATGCATTTGGCAGGAAGTGACAGTGTGAGAAGAATGCACATAGACCCTTTCCTTACCCCCATGTCGACG AAAGAAAGGAGTAAAGCCTTCAGTTTGGCAGATGTGGATAAGAGGTCTCCGGAGAAGAAAAACTGTTACA CACGGGCTGGGGTGTGTTCTTGAATGACTGTGCAGGCGTCATCCCTAAGCCAAGCTCAGAAGGCCACTTC CTGACTGGCAGAAGTTGTTTCCCAAAGCACTTCGTACAGAGAAGGCAGAAGCTAGACTTAGAAAAACTGA ATGTTAGATCAAATACTGCAAGTTTAGTGCAGAAGCCCTCTAGTTTGAGTTCTGGCCTGGTCTTTGAGAG CACAGCTTCTCTGTTGCAAAGCTGACACTCTGTAAATTTAGTTCAGGAGGTATGTGTTCTAAAATGCTCT TACTTAGGAAAGTAACTGCTGGCCATCAGCTTTGCTGTCCTGCCTCTGGCTCTTTTCCTTCATGCTACTA GGTGGCTGAGCCACGACCTCCCACCTCATCTCCCCATCAGAGTCTTCAGACCTACTCACTGCAGGCAGAC GGGTCACTTAATCCCTCATTTATGCAGGCTTAACCAATCAGCTTCCAGTCATAGCCAAACGTAAGCTCAC TTGGGAGTATCTTACTGCTCTTGAAAGAGCTGCTTTTCTATCTCAAAGCTTTGCTTGCTCTTCTGCTGTC CTCACTAGTCATTCCGTGCCATCAGGAGACAACATAGGAGCCACTGCCGCAGAGTGACGGGTGGCCATTG AGTTGTCAGGGCCCGAGTCCTCTAAACTAAGCTCATTCTCAGCCAAGGAATGGACTCCCTTGCCCTCAAA GCCCCCTACAGTGTTCCTGTGGATGCTGGCCATGAGCTGCCTGTTTCAGGAAATCCACAAGCAGGGTCAA GCTTGGACCAGCATGGAGGTAAGCCCATGCTAAAACCCCATCACTAGTGAGTGAAGCTAGAGGACCATTC AAGACCAGCCTGTGCTACATAGCAAGACTGTCTTGAAAGAAAGAGAAAGAAAAGAAAAGAAAGGAAACAA GCTTGTCTTATGTGTGCTCACACACACATATTAAATGCTGGTTCTTTTCCCTGAATAAAGAAAGCACTGA TTTCTGAATCTCAGAGAACCTAAATCTAAAACCAATGGCCTTTCCTAAAAAAGACTTAAGTGAACAGTTG ACTGGTGGGTGTGTTGTGGTGACTCATCTTAGACCTTCCACTGGATCACGGTATTTGACCGTGTCAGTCT GCATAGTTGCCCTGATTGGTAGTTTGTTATCTGTTTTGTACACTTCTGTTACAAGTGAAGATTTTCTACA ACTAAGGCAAACGACCAGTTGAAATGAACTACAGGAACCTGAGAAAATGTTTATGTATAGCCTGAAGTTG AAAAGGGATAATAATCTATTTATTGATACTGTTGTTTATGTTGTTATCTCTCAGAGATGCATGTTGTGCT ATAACATGGTACAAGGAATAAAAGTTTATCAGAAGCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6417473 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6417473 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7666 same embryo
	EMAGE:7670 same embryo
	EMAGE:7668 same embryo
	EMAGE:7669 same embryo
	EMAGE:7671 same embryo
	EurExpress:euxassay_005294 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source