EMAGE mouse gene expression spatial database

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EMAGE:7670

Pde12 phosphodiesterase 12 ( MGI:2443226)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005279. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005279_01	euxassay_005279_02	euxassay_005279_03	euxassay_005279_04

euxassay_005279_05	euxassay_005279_06	euxassay_005279_07	euxassay_005279_08	euxassay_005279_09

euxassay_005279_10	euxassay_005279_11	euxassay_005279_12	euxassay_005279_13	euxassay_005279_14

euxassay_005279_15	euxassay_005279_16	euxassay_005279_17	euxassay_005279_18	euxassay_005279_19

euxassay_005279_20	euxassay_005279_21	euxassay_005279_22	euxassay_005279_23	euxassay_005279_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7670_wholemount_strong.wlz

7670_wholemount_moderate.wlz

7670_wholemount_weak.wlz

7670_wholemount_possible.wlz

7670_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7670_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8928
Entity Detected:	Pde12, phosphodiesterase 12 ( MGI:2443226)
Sequence:	sense strand is shown >T8928 CCCTCGGGCACTCTTATATTTCCTTCACTCTTCGCCTTTTACCCATGTTGAATAAAAACACCAAGGCGCT CGAAACTCGAGACCGGCGGTCAAGACAAGAAGGAGCTGGCAAGTGGCCTTGGCGAAAGTGCGAGTCGGGA ACACCGCGCGCTTGATGCTCACACCCCGGCTTCTGCTTGCCGCCGACCGGAAGTCCACGGTCTGAATGGG TCTTGTACCCGGCCACGGCCGCTCCTCCCGGTGGACTTGACAGCGGGCGACTCTGGATCTCTGATCTCGT CGGCTGCCAGGTTCATGTGGAGGCTCCCGGGCCGCAGCGCGCTTCGTGGGGTCCGGTCGGTGGTGGAGCG GCGCAGCCGGGCCGAGGCAGGGACTCACGAGGCGGTGCGCGCCATGGAGCGCGCGGTGGTGCGCTGTGTG CCCTCGGAGCCCAAGCTCAGCCTGTCTTTCGCGTTGGCCGACGGCAGCCACAAAAACATGCAGAGGGACC AGAGCGAGCCTCTGGGCCGGGCCCTCAGCCGGATCGCTACCAACGCCCTCAAGGGACACGCAAAGGTAGC GGCCGCCAAGAAGAGCAGGAAGAACCGAGCCCACTCGAGCGGTGGCGCGGCCTGTGAGGCTACAGGGCCT GAGCCGGTTGCGACCTGCGAGCCGGTGGTGAAGCTGTACTACCGGGAGGAGGCGGTGGCCGAGGATGTGC TCAATGTGGACGCCTGGCAGGACGGAGCGGTGCTGCAGATCGGCGACGTCAAGTACAAGGTGGAGCGCAA CCCGCCCACCTTCACGGAGCTGCAGTTGCCGCGCTACATCATGGCTGGCTTCCCTGTGTGCCCCAAACTC GGCGTGGAATTTGGGGATCCCGCCAGCTCCGTGTTCCGCTGGTACAAGGAAGTCAAACCCGGGGCGGCAG AACCTGGGGACAGCGGCCCCGCGTCCTCGTCTCACTCTTCGCAGCCTTCAGCTTGGATCGAGACCGGTGT GGACGAGCGCGTCTACACCCCGTGCAATGCAGACATCGGGCTCCGGCTCCGGCTTCACTGTACACCGGGC AATGGGCAGCGCTTCGGGCCAAGCCGGGAGCTAGAAAGCCTGTGCCCGGTGGAGGCCGGGCCTGGCACCT GCACTTTTGACCATCGACATCTGTACACCAAGAAGGTGACGGAGGACTCTTTCATCCGCACTGTCTCCTA CAACATCCTGGCAGACACGTACGCCCAGACTGAGTTCTCTCGGACGGTCTTGTACCCGTACTGTGCCCCC TACGCCCTGGAGCTTGACTACCGCCAGAATCTCATACAGAAGGAGCTCACAGGCTACAACGCAGACCTCA TCTGTTTGCAGGAGGTGGACCGCGCGGTGTTCTCCGACAGCTTGGTACCGGCCCTGGAGGCCTTCGGGCT GGAGGGCGTGTTCCGAATCAAGCAGCACGAAGGCCTGGCCACTTTCTACCGGAAGTCCAAGTTCAGGCTC CTTAGTCAACATGACATCTCTTTTCAAGAAGCCCTCAAGTCGGACCCACTGCACAAAGAATTACTAGAGA AGCTGGCTTTGAACCCATTAGCACAGGAGAAGGTCCTCCAGAGGTCATCTGTCCTTCAGATTTCAGTTCT TCAGTCTACAACAGACTCTTCCAAAAAGATATGTGTTGCTAATACCCATCTCTACTGGCACCCAAAAGGT GGATACATTCGTCTCATTCAAATGGAAGTAGCCTTGGTTCATATTAGACATGTCTCACGTGATCTCTATC CTGGCATACCAGTTATATTTTGTGGAGACTTTAATAGTACACCATCAACAGGCATGTATCATTTTGTCAT CAGTGGCAGCATTGCAGAGGATCATGAAGACTGGGCTTCCAATGGGGAAGAGGAGAGATGCAGCATGCCT CTCTCACACTGTTTCAAACTGAAGAGTGCTTGTGGTGAACCTGCCTACACAAATTATGTCGGTGGCTTTC ACGGATGTCTAGATTACATTTTCATTGACTTAAATACTTTAGAGGTTGAACAGGTGATTCCATTACCTAG TCATGAAGAAGTTACCACCCACCAGGCCTTACCTAGTGTTTCTCATCCTTCTGATCACATAGCACTTGTA TGTGACTTAAAATGGAAATAGATTTTACTTCACTAACTCTGAAAGGAAGCTAGGAACTGTCCAAAAAAAT GTAATATGAAACAAAGCCCATATATCAACCTTCAGTGAGGCAACACCAGACTCAATGGTCATTCCCTACT GGTTATGTTCCAGACGGCTTTGTTACAGAACTAATTCATCTTTGCATCATATAATATCGTTATCCTCCTT TTTAAAATTCCCTACATTTAGAGGAAAACATATTTATGACTAGCAAGAAGAAAATTGAATTATTGTGTAC ATTTTATAAGTTTTTTTAAGCTGGTAATTACGACTTTTAAGTACTATTTGAATAATCTCAATTTTTCTTT CACAGCACATTTTACATTGAATTATGTTAATATAATTGGGGAAGGAGGGGACATGTAGGTAAAACGTGAG AGACTTCAGAGACACATGGAATACATTAGTCTGTCACTACCTCAATTTGGTTAGAATTTATTATAATTTT GGAGCTTTAACAAAAGATTAAAATGTACCATTACCAATTCTTATAGCTTCTTCGTTTTTCAGTTAGCAAG GTATTTTCCTTCCTGCGTTGTTTTCAGTGTACAAACGACGGTTCAAACGCGTCTACTTTGTCTTCTTACT TGCACTTGAGCTCATTTTAATAGTAGGATACAAGCACAAAAGAGTATTCAAAGGGACGGGGACTTGACTT GATCCCTTGCCTTTGCTCTTACTCATAAAATATAAGCACACAGGGAAAAGGGGAAGCAGGCATGGTGCTT TATAGCTGTAATCCTAGTGCTCAGGTACGTGAGGCAGGACTGCGTCGAGGTCAGTTCTGGTCTGGGTTAC ACAGTTCAAGGCCAGCTTGGGCTACATACATAGGAAAAAAGGGGACAATGGGTTTTTGTCAATATCTGAA ATAAACTTTTTATAGATGTAAGCATTATAGCTTGAGGAACAGAATAGTGCCACATAATTTATATAAAAAA TTTGAAGATGAAGCCAACATTTGAAGATGAGAAATTCCAAGCTGTGTTGATTAGTATCTAGTTTAGATGC TTTTACTGCATTTGTTCTCACCATGTCTGCTTGGTGATGCTTTAGCGGTTGCTTTGTCATGTCCAGGTTT AACTGTAGCATAAGCATAGCTTTCTGCACTTAATTTCTAGCATAGATAATAAAGGCCTCTTACCTCAAAA AGAGGTCTTATTTTCCAAGTCTTCAAATTATTTAATCTGTAAATCCATTTTTTCCCCATATTCTCTAATG TGATCTGTGTTTTCCATATTCTGTTATCAGATACTCTTAGGTTAGTAAGTCTGAAAAATATAAGCATTTC ATTTGCACTTCACATTGAACCTTTATTCATATTATTTAAAGGGAAATATAAAATGACAGTTTCTTGGGAT GCTTAAAATAAAAAAGCTGATGAAAAGTTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6417852 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6417852 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7666 same embryo
	EMAGE:7668 same embryo
	EMAGE:7667 same embryo
	EMAGE:7669 same embryo
	EMAGE:7671 same embryo
	EurExpress:euxassay_005279 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source