EMAGE mouse gene expression spatial database

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EMAGE:7668

Rnf41 ring finger protein 41 ( MGI:1914838)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005289. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005289_01	euxassay_005289_02	euxassay_005289_03	euxassay_005289_04

euxassay_005289_05	euxassay_005289_06	euxassay_005289_07	euxassay_005289_08	euxassay_005289_09

euxassay_005289_10	euxassay_005289_11	euxassay_005289_12	euxassay_005289_13	euxassay_005289_14

euxassay_005289_15	euxassay_005289_16	euxassay_005289_17	euxassay_005289_18	euxassay_005289_19

euxassay_005289_20	euxassay_005289_21	euxassay_005289_22	euxassay_005289_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7668_wholemount_strong.wlz

7668_wholemount_moderate.wlz

7668_wholemount_weak.wlz

7668_wholemount_possible.wlz

7668_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7668_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8938
Entity Detected:	Rnf41, ring finger protein 41 ( MGI:1914838)
Sequence:	sense strand is shown >T8938 GGGCTAACCCGAGACAGCGAGGAGTGGTCGGAACCTTCTTCCCCATCCTTACCTTCCCCTTCCCCTACGG GGAAAGGCTGAGACCGGGCACACAGTGTGCTACGCGGCCGCCGTGTCCTCAGCTGCAGCCAGGCCCCGCG TCCGGAGAAACCAGGCTGCGTTCCGGGTGAGCGTTCCTGATTGTGACATCACACCCACCTCTGGGCCATG AGACTTGTCACTAGGAAGAAAGACTCAGTTGGAGAATAGCCAACAAGATGGGATACTCAGAGCATCTCCT GAGCGGCACTGAGCGGAGGCACCAGGGGGTTGGAACCTTGTGAACAGGGAACCTGCCCCCACCACTTAAA AGGACCTGGGTTTCAGTGATGAGACATGGGGTATGATGTGACCCGTTTCCAGGGGGACGTTGACGAGGAC CTTATCTGCCCTATTTGCAGTGGAGTCTTGGAGGAGCCAGTGCAGGCGCCTCATTGTGAGCATGCCTTCT GCAACGCCTGCATCACCCAATGGTTCTCTCAGCAGCAGACGTGTCCGGTAGACCGTAGCGTTGTCACGGT TGCCCACCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTACAGATTGCCTGTGACAAC GCTGTGTTTGGCTGCAGTGCTGTTGTCAGGCTTGACAACCTCATGTCCCACCTCAGTGACTGTGAGCACA ACCCGAAGCGGCCTGTGACCTGTGAGCAGGGCTGTGGCCTGGAGATGCCCAAAGATGAACTGCCAAACCA CAATTGCATTAAGCACCTGCGCTCCGTGGTCCAGCAGCAGCAGTCGCGCATTGCAGAGCTGGAGAAGACC TCGGCTGAACACAAGCACCAGCTGGCAGAGCAGAAGCGAGACATTCAGCTGCTGAAGGCGTATATGCGAG CCATCCGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATCGAATACAATGAGATCCTCGAGTG GGTGAACTCCCTGCAGCCGGCAAGGGTGACCCGCTGGGGGGGCATGATCTCCACTCCTGATGCTGTGCTC CAGGCTGTCATCAAGCGCTCCCTCGTGGAAAGTGGCTGCCCGGCCTCCATCGTCAACGAGCTGATTGAAA ATGCCCATGAACGCAGCTGGCCCCAGGGTCTGGCCACACTAGAGACAAGACAGATGAACCGGCGCTACTA TGAGAACTACGTGGCCAAGCGCATCCCTGGCAAGCAGGCTGTAGTGGTGATGGCCTGTGAGAACCAGCAC ATGGGGGACGACATGGTGCAGGAGCCAGGGCTCGTCATGATATTTGCGCATGGTGTGGAGGAGATATAAG AGCGTAACTGGCTGTCAGGAAGAGCATGAGCTCTGGTCTGATACCTGCTCTGACCCTCCCCTCCATCACC CTGCAGGAGCTTTGGCTGTGGCAGTCCAGCGTTGATGCCTGTGACCCATGAAGAAATTTGTTCCCTGCCC CTTTAGGTCTCCTTGTGAAACCCTAGAATTAACTGGCTGGCACATGTGTTTTGAAGGTGACTCTTCCATG TTTCCTGAGGAAACTTCCTGTTTATCTGGGTTCTAACCTTCACCAGCCAAGTGGGATATAAATCAGATGT CTCCAAAGCCAGGCTGACTACACTGGCTCAGTCAAGTCCACCCAAGGACTTGGGGTCCATTCAAGGCTCT CTGGGTGGCTGCTGGGTCCCCCATGGTGGCTAGTAGAGTGAGCAAGGAGAAAGAGTGGTGTGATTATTTT AAAAGCGTAGGTGGGAGTGTGATTGGAGAGGTCAGAGGGCCCCCCTTCACCATACTCTAGCTTCTCTCTG GGAGTTCCACTTCAGGCTAGCCTGCTGTTGGTCTCACTGGTTGAGTCGTGTGCCTCCTGTGGGGTTGGGA TAGAAGGATATAGCTTGAGGACCGTAAGAAAAAGTTGGGTGTTCCAGCTCCAGGACATTGTGCTCAGGAG TTTGAAAAAGGCTGCTATACTCAGTCGGGTTACTGGGTTTCTTGTTCCTCTTTGTGCCTGCCTGCCTTGC CCAAGCTCACTCTGTCTATTCCTTACTCTGAGATGAATGATGGAGGAGGCTGAAGTTTGCTTGCCCTTTG CACCAGTATCTGCAGCTCTTCCATTGTGCTGTGTGCTGTACATGGTGTTCACTGCACTAATAAATCTCTC ACTCAACTCTAAATTCTTAGTCACCCCCCTTATGAAGGATTCTTCCTAGCTTGGTAATGCACCTTCCACT GACCTCTGCCTTGAGAGACCGTCTGTCTGGAAACTGCATACACTTCACACGCTTCACGTTCTGTGCTCTA CTGCTCTCGTAAGCCTAACTGCATTCTCTTCAGAGTGTCCCTTTAGCCGGAATTCTGTTTTCCTAAATGT CCTGCATTCGGAGGATGCTACCGAAGACTTTAGCACCTCTTTCCAGCGAGTGGGGAGCTTCTGCCCTCTA GGGGCTCCTGATATTAATGTAGGGAGGAAACCTGAAACAGCTCTCCTGCTGTTCAGACCTTGACAAGGAC TTTCCTGGCTAGGGAGATGGCTAGGGAGAGGTAAGAGCTCTAACTGCACGTGAGGATGTGAGTTCAAATT CCCAGCACCTGCATAAAAAGCTGAGCATGGTCAAGAGATAACTCCCTCGTTGTGTGGGGCAGAGGTAGAG GGCTTACTGGAGCTTGCTGGTCCCTATTTGAGCTCCAAGTTCAGTGACTGACAGTGTCTCAAAGAATGAA GCCAGGTATGGTGGAACATGCCTTTAATCCCAGTACTTTGGAGCCAGCAGACAGATCTCAGTGAGGGGCC TGCAAGTTTAAAGGAATATGGCATAGTAATAAAACAGGACACCCAACATCCTCTTCCAAACACCTTGAAA GCATGAGTATGCACAATGCACATACATGTCCACCATGCTCACACATTTTTGCTGAACACTTTTTGTTACT ATCTTATTCCTCCTATGAGAAACCATGTGTGTGTGTGTGTGTGTGTGTGCATGTGTGACTTAAGATATTT ATGTATATTAATGTTTTGCCTGTATTTCTGTCTGTGTGCCACATGTATGTCTGTTGCCCACAGAGTCTAG AAAAGAGCACTGGGTCTCCTAGAACTGGAGTCACAGAAGGTTGTGAGCCACCATGTCGAGTTTGAAACTA GGTGCTATAGAGCAGCCAGTGCTCTTAACCACTGAGCCAGCTCCCAACTGTATGTTGAATTTGTTGACCT TTATCGTGCACCGAATGTAAATATAATGGAACTCTCCTGACACTTAAGTTGAGGGAAAGGACAAAAAAAA AAAAAAAAAATTC
Notes:	The probe template was PCR amplified from IMAGE:6419123 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6419123 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7666 same embryo
	EMAGE:7670 same embryo
	EMAGE:7667 same embryo
	EMAGE:7669 same embryo
	EMAGE:7671 same embryo
	EurExpress:euxassay_005289 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source