EMAGE mouse gene expression spatial database

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EMAGE:7673

Morc2a microrchidia 2A ( MGI:1921772)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005286. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005286_01	euxassay_005286_02	euxassay_005286_03	euxassay_005286_04

euxassay_005286_05	euxassay_005286_06	euxassay_005286_07	euxassay_005286_08	euxassay_005286_09

euxassay_005286_10	euxassay_005286_11	euxassay_005286_12	euxassay_005286_13	euxassay_005286_14

euxassay_005286_15	euxassay_005286_16	euxassay_005286_17	euxassay_005286_18	euxassay_005286_19

euxassay_005286_20	euxassay_005286_21	euxassay_005286_22	euxassay_005286_23	euxassay_005286_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7673_wholemount_strong.wlz

7673_wholemount_moderate.wlz

7673_wholemount_weak.wlz

7673_wholemount_possible.wlz

7673_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7673_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8917
Entity Detected:	Morc2a, microrchidia 2A ( MGI:1921772)
Sequence:	sense strand is shown >T8917 CTTGCTGAACTGGTTGATAATGCAAGAGATGCTGATGCTACCAGAATAGACATTTATGCTGAGGAAATGG TCCAATATGGTCAACCTGGCACCAGGTCACTGTTTCTAGTCACACCAGAGATGATGCCGTATCTGAGCCT CAGTGTTGATCTCTGTGGTTGGCAGATCTGGCACTCAGCAGTGGCTGTAGCCAGGTCACTTGTTGAATGA GAGGCGGGAGGACCTTCGAGGAGGATTTATGCTTTGCTTTTTGGATGATGGAGCAGGAATGGATCCAAGT GATGCTGCTAGTGTGATCCAGTTTGGGAAGTCAGCCAAAAGAACTCCTGAGTCCACTCAAATTGGGCAGT ATGGCAATGGATTAAAATCGGGCTCAATGCGGATTGGGAAGGATTTTATCCTTTTCACTAAGAAGGAAGA TACCATGACCTGCCTCTTCTTGTCTCGCACCTTTCATGAGGAAGAAGGCATTGATGAAGTAATTGTCCCA TTGCCCACCTGGAATGCTCGAACACGGGAACCTATCACAGACAATGTGGAGAAGTTTGCCATTGAGACAG AGCTTGTCTACAAATACTCTCCCTTCCACACTGAGGAGCAAGTGATGAACCAGTTCATGAAGATTCCTGG GAATAGTGGAACACTGGTGATCATCTTTAATCTCAAGCTCATGGATAATGGAGAGCCGGAACTAGACATA ATCTCAAATCCAAAAGATATCCAGATGGCAGAAACCTCGCCAGAGGGCACGAAGCCAGAGCGACGCTCCT TCCGAGCCTATGCTGCTGTACTCTATATTGATCCCCGGATGAGGATCTTCATCCATGGACACAAGGTGCA GACCAAGAGACTCTCCTGCTGCCTATATAAGCCCAGGATGTACAAGTATACATCAAGTCGTTTCAAGACT CGGGCAGAACAGGAAGTGAAGAAAGCAGAGCATGTAGCACGGATTGCTGAAGAGAAGGCCCGGGAGGCAG AGAGCAAAGCTCGTACTTTAGAAGTACGCATGGGTGGAGACCTGACACGGGACTCCAGGGTGATGTTGCG GCAGGTCCAGAATACAGCCATCACCCTTCGTAGAGAAGCTGATGTCAAGAAAAGGATCAAGGATGCCAAG CAGCGAGCTCTCAAAGAACCCAAGGAACTTAATTTTGTTTTTGGGGTCAATATTGAACACCGGGACTTAG ATGGCATGTTTATCTACAACTGTAGCCGCCTGATCAAGATGTATGAGAAAGTGGGCCCACAGCTGGAAGG GGGCATGGCATGTGGTGGGGTTGTTGGGGTTGTTGATGTACCCTACTTGGTTCTGGAGCCTACACATAAC AAGCAGGACTTTGCTGATGCTAAGGAGTACCGGCATCTACTTCGAGCAATGGGGGAGCACTTGGCACAGT ACTGGAAGGACATAGCCATTGCCCAGCGTGGAATCATCAAGTTTTGGGATGAGTTTGGCTATCTTTCTGC CAACTGGAACCAACCTCCATCTAGTGAGCTGCGTTTCAAACGCCGGAGAGCTATGGAAATTCCAACCACT ATCCAGTGTGATCTGTGTCTGAAATGGAGGACTCTCCCCTTCCAGCTGAGTTCAGTAGAAACAGATTACC CTGATACTTGGGTTTGCTCCATGAACCCTGATCCTGAGCAGGACCGCTGTGAAGCTTCTGAACAGAAGCA AAAGGTTCCTTTGGGGACATTAAAAAAGGACCCAAAGACACAGGAAGAAAAGCAGAAGCAGCTGACAGAG AAAATTCGCCAGCAGCAGGAAAAGCTAGAAGCTCTTCAGAAAACCACGCCCATCCGCTCACAAGCTGACC TGAAGAAATTGCCCTTAGAAGTGACTACAAGACCTATTGAAGAACCTGTACGGAGACCTCAGCGTCCTCG GTCACCTCCTTTACCTGCGGTGATCAAGAATGCTCCTAGCAGACCCCCTTCTATTCAGACTCCCAGACCA AGTACCCAGCTCCGAAAGACGTCTGTTATCAGCCTTCCAAAACCACCCACTACAGCAGCCCGAGGAGAAA CTAGTACTTCTAGGCTACTCCAGCCAACTGAGGCACCTCGAAAGCCTGCAAACCCTCCCATCAAGACTGT ACCCCGACCCACCCCTCCTGTGCACACACCCCCACTATCTTTAATCCCCAGTTCCAAGAGCCTTCGGGAG GTCCCTGCTCAAAAAGCCATCAAAACTCCAGTGGTCAAGAAGCCAGAGCCACCTGTCAAACAGTCCGTGG CAACCTCAGGTCGGAAGCGGAGTCTTGCAGTCTCAGATGAGGAAGAGGCTGAAGAAGAAGCTGAGAAGAG GAGAGAGAGATGCAAGCGGGGCAAACTTGCTGTGAAGGAGGAAAAGAAGGAAGCAAATGAGGTGCTCTCA GACAGTGCTGGGGAAGATCACCCAGCTGAACTCAGGAAGGCTCAGAAAGATAAAGGACTTCATGTAGAGG TCCGTGTGAACCGTGAGTGGTACACAGGCCGAGTCACAGCTGTGGAGGTGGGCAAGAATGCGGTTCGATG GAAAGTGAAGTTTGACTATGTGCCCACAGATACAACACCAAGAGACCGCTGGGTGGAGAAAGGCAGTGAA GATGTACGATTGATGAAGCCACCTTCCCCAGAGCATCAGAGCCCTGACACACAGCAAGAAGGTGGAGAGG AGGAGGAGGCTATGGTGGCCCGGCAGGCTGTGGCCTTGCCTGAGCCTTCCACCTCTGATGGTCTTCCCAT TGAGCCTGATACCACTGCCACCAGTCCCAGCCATGAGACTATTGATCTCCTGGTCCAGATTCTTCGAAAT TGCTTGCGGTATTTCTTACCTCCAAGTTTCCCCATCTCCAAGAAGGAGCTGAGTGTTATGAATTCAGAAG AACTAATATCTTTTCCTCTGAAAGAATACTTCAAGCAATATGAGGTGGGGCTCCAGAATCTGTGCCATTC CTACCAAAGCCGTGCTGATTCACGGGCAAAAGCCTCAGAGGAGAGCTTACGCACATCAGAGAAAAAGCTT CGTGAGACAGAGGAGAAGTTACAGAAGTTGAGGACCAACATTGTAGCACTCCTGCAAAAAGTGCAGGAGG ACATAGACATCAACACAGATGATGAGCTGGATGCTTACATTGAGGACCTCATCACCAAAGGGGACTGAGG AGCCGGGAGGCTAACTCCTGCTCATACCTGCCCTCAACACAGCAGCTTCGGGGAGGGGAGTTCATTTGTG ACTTGGTGGACTTGATCTTGGTTTGAGTCATGTGAGACATTTGTGCTTTGGGAAGGAGATTCTTTGAATC TTCTTCCCTAAGTTTACATAAATATTTATTTTTTAAAAGAGAAGATGTTAATGCCTGCTGTGAGACCGTA CTTTTTTTTGTGTGACTCTTGAAAGGGCACAGAGTCTGAACCATTCAGCTCCTGGCACCTCAACATAGGA TGAAGAAAGCACCATCCTGAAGTTGGAGAGGCTCTAAGGACATCAACAATGGGATATGAAAGATACATGG CCCCAGGGACTCAGCTCTGCAAGACCCTCAAGGGGTGATCAAAACAGGCCCAGTATCAGAAAGACACTCC CTGTAACAGATCTCCTGGTGTCCAGACAGGGCTGTGAAGGTCTGTAGGCTGCCTGAGCAGCTGTCAGTCA TCTTGGATGTGACAGTATCCAGACAGCTTGGAGATGTTTCTTAAGGCTCAAATTTCCCAATATGCCACCT ACTTTGTTACTGGAGGAGCCATTTCCTGAGTCAACCACACTATCCAAGCTCAAGCATAGACAGCTCTGAC CGGTCAGATCCCTGGGCTGGATAGCACTCTGAATGTTGAACTCCAAAGATTCTCAGGGTGTGCAGGTGTG AAAGCTCTCTGGAGGAGAAGGCTTCCACAGTGGTGCTAGTTCCTGGAGAGCCTTTGCTGCTGCTTTTCTA CTATGTACTCTCTGGGCTCCAGAGAGCCCTGCACCTCCCATGCTCTGGAGGTGAGGAGGACAGACCATGT GCTTCAGACAACCACTCAGAGCCAGAAAGGCACGAGCTTCATCTGATGGCTGTCGGAAGAATTCTGGTTC CAGTCCCAACTCTTCACACTCCTGTTCAAGACTGATTTTGTACTCTTGATCCAAACCACCTTGGGATTTG TATCCATCAAGGCAAGGCTGCCACCTTGTGTCCTGGGGTCTCTGCAGAAGATGGGGAAGTGTTGGTGAGG GAACTGTGCACATATGGCTGGAAGTGCCTGCTTGAGTTCTGCCATCAGGTTGTTTAGGGGGGATAGCATT CAGAAGTTCTTCTCTGGAACATTCTCCAATAGATGGGTTTTAAGTTGGTGCACTTACCCCCACGGCTGCC CCCATCCAACTTTCTGTTGACCCTATCCAGGCAGAGTATGATCCATTTTTTTCCATAAACCCACTGTTTA AAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6417551 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6417551 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7672 same embryo
	EMAGE:7674 same embryo
	EMAGE:7675 same embryo
	EMAGE:7677 same embryo
	EMAGE:7676 same embryo
	EurExpress:euxassay_005286 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source