EMAGE mouse gene expression spatial database

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EMAGE:7677

Lrpprc leucine-rich PPR-motif containing ( MGI:1919666)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005284. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005284_01	euxassay_005284_02	euxassay_005284_03	euxassay_005284_04

euxassay_005284_05	euxassay_005284_06	euxassay_005284_07	euxassay_005284_08	euxassay_005284_09

euxassay_005284_10	euxassay_005284_11	euxassay_005284_12	euxassay_005284_13	euxassay_005284_14

euxassay_005284_15	euxassay_005284_16	euxassay_005284_17	euxassay_005284_18	euxassay_005284_19

euxassay_005284_20	euxassay_005284_21	euxassay_005284_22	euxassay_005284_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7677_wholemount_strong.wlz

7677_wholemount_moderate.wlz

7677_wholemount_weak.wlz

7677_wholemount_possible.wlz

7677_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7677_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8918
Entity Detected:	Lrpprc, leucine-rich PPR-motif containing ( MGI:1919666)
Sequence:	sense strand is shown >T8918 CTGAGCCATGGCAGCCCTGCTGAGACCCGCGCGTTGGCTGCTCGGGGCCGCGGCGGCCCCGCGCCTCCCG CTGTCCCTGCGCCTCCCTGCGGGCGTCCCGGGCCGGCTGTCCTCCGTCGTCCGGGTCGCGGCTGTTGGTA GCCGGCCGGCTGCAGGAGAGCGTCTGAGCCAAGCCAGATTGTATGCCATCGTTGCTGAGAAAAGGGATCT TCAAGAGGAGCCTGCTCCTGTGAGAAAGAACAGCAGTCAATTTGACTGGGCTCTGATGAGACTGGATAAT TCTGTCCGGAGAACAGGCCGCATCACAAAGGGGCTTCTGCAGAGAGTCTTTGAGAGCACGTGTAGCTCAG GTAGCCCAGGGAGCAATCAAGCTCTGCTTCTGCTGCGCAGCTGTGGCTCGCTCCTGCCCGAACTGAGTCT CGCCGAGAGGACAGAGTTTGCTCACAAGATCTGGGACAAACTTCAGCAGTTAGGTGTCGTATATGATGTC AGTCATTACAATGCTTTACTTAAAGTATATCTTCAAAATGAATACAAATTTTCACCTACTGACTTCCTGG CAAAGATGGAGGGAGCAAACATCCAACCAAATCGAGTAACATACCAGAGGCTGATAGCTGCCTACTGTAA TGTTGGGGACATTGAAGGTGCCAGCAAGATCCTTGGATTTATGAAAACGAAAGACCTTCCGATCACAGAG GCCGTGTTCAGTGCTCTCGTCACAGGGCATGCGAGAGCTGGGGATATGGAAAATGCAGAAAATATTCTCA CAGTGATGAAACAGGCCGGCATTGAGCCTGGCCCAGACACGTATCTGGCCTTGTTGAATGCACATGCTGA GAGGGGTGACATTGGCCAGGTTAGGCAGATTCTGGAGAAAGTGGAGAAGTCAGACCATTACTTCATGGAC CGCGACTTCTTGCAGGTTATTTTTAGCTTCAGTAAGGCTGGCTACCCTCAGTATGTCTCAGAAATACTGG AGAAGATTACCTATGAGAGACGGTCTATTCCAGATGCAATGAACCTCATTTTGTTTTTAGCCACTGAGAA GTTAGAAGACACTGCGTTCCAGGTTTTATTGGCATTACCCCTGTCCAAGGACGAGAGCTCCGATAACTTT GGCAGTTTCTTTTTGCGGCACTGTGTGACTCTGGATTTGCCCCCTGAGAAGCTGATAGACTACTGTCGGA GGCTGAGGGACGCCAAGCTGCACAGCTCCTCACTGCAGTTCACGCTGCACTGTGCTCTTCAAGCCAATAG GACAGCTTTGGCAAAAGCAGTGATGGAGGCTTTGAGGGAAGAAGGGTTTCCTATCCGACCGCACTATTTC TGGCCGTTGCTTGCTGGGCATCAGAAAACAAAAAATGTTCAAGGAATAATAGATATCCTCAAAATAATGA ACAAAGTGGGAGTGGATCCTGATCAGGAAACATATATAAACTATGTGTTTCCGTGCTTTGATAGTGCACA GTCAGTTCGAGCTGCTTTGCAGGAAAATGAATGTCTCCTCGCAAGTAGTACCTTTGCTCAAGCTGAAGTG AAGAATGAAGCAATAAATGGGAACTTACAGAACATTTTGTCATTTTTGGAATCGAATACATTGCCTTTCT CGTTTAGTTCTTTGAGAAACAGCCTAATCCTAGGCTTCAGGAGGTCGATGAACATAGATCTTTGGAGCAA GATAACAGAATTGTTGTACAAGGATGAACGCTATTGCTCAAAGCCTCCGGGACCAGCGGAAGCTGTTGGC TATTTTCTTTATAACTTGATTGACAGCATGAGTGACTCAGAGGTACAGGCCAAGGAGGAGCGTTTGAGAC AATACTTCCATCAGCTGCAGGAGATGAATGTTAAAGTTCCTGAAAACATCTACAAAGGCATTTGTAATTT GCTGAATACCTACCATGTTCCTGAATTGATTAAGGATATTAAGGTTCTGGTTGACAGAGAGAAGGTAGAT TCTCAAAAAACTTCTCAAGTTACCTCATCTGATTTGGAATCAACACTTGAGAAACTCAAAGCTGAAGGCC AACCTGTAGGATCTGCCCTGAAGCAGCTCCTGCTGCTGCTCTGCTCAGAGGAGAATATGCAAAAGGCCCT TGAGGTGAAAGCAAAATATGAGTCAGACATGGTTATTGGTGGCTATGCAGCATTAATAAATTTGTGCTGT CGACATGATAATGCAGAAGATGCGTGGAACTTGAAACAAGAAGTTGACCGCTTAGATGCTTCGGCTATTC TTGACACTGCCAAGTACGTAGCCCTTGTAAAAGTACTGGGAAAGCACAGCAGACTCCAAGATGCTATTAA CATTCTAAAGGAGATGAAAGAGAAGGATGTTGTTATCAAAGATGCAACAGTCTTGTCCTTTTTCCACATC CTCAATGGTGCAGCTTTAAGAGGTGAAATTGAAACAGTAAAACAGCTGCATGAAGCCATCGTGACTCTTG GGTTGGCAAAGCCGTCCAGCAACATAAGCTTCCCGTTGGTCACTGTGCACCTGGAAAAGGGTGACTTACC TGCTGCTCTTGAAGCCAGCATTGCCTGCCATAAAAAATATAAAGTGTTACCCAGGATTCATGATGTCTTA TGTAAGCTAGTAGAGAAAGGCGAGACTGATTTGATCCAGAAAGCAATGGACTTTGTGAGCCAAGAACAAG GGGAGATGACGATGCTCTACGACCTCTTCTTTGCTTTCCTGCAGACGGGGAATTACAAAGAAGCTAAGAA GATCATTGAGACTCCAGGCATTAGAGCTCGGCCTACAAGACTCCAGTGGTTTTGTGATCGATGCATTGCC AGTAATCAGGTTGAAGCTCTTGAGAAGTTGGTAGAGCTGACTGAGAAGCTGTTTGAGTGTGACAGAGACC AGATGTACTACAACTTACTGAAGCTATACAAAATAAGCAGTGACTGGCAAAGAGCGGATGCTGCGTGGAC CAAAATGCAAGAAGAGAACATTATCCCTCGAGAGCGGACACTGCGGCTCTTAGCCGAGATCTTGAAAACC AGCAACCAGGAAGTTCCTTTCGACGTTCCGGAGTTGTGGTTTGGAGATGACAGACCTTCCCTGAGTCCAT CCTCACGCTCAGCAGGAGAGGACGTTACTGAGAAGACGTTGTTGTCTAACTGCAAACTAAAGAAGAGTAA AGATGCATATAATATCTTCCTTAAAGCCGAAAAGCAAAACGTTGTATTTAGCAGTGAAACTTATAGCACC TTGATAGGCTTGCTGCTGAGTAAGGACGACTTCACCCAAGCAATGCACGTGAAGGATTTCGCTGAGACCC ACATCAAGGGCTTCACACTGAACGATGCTGCCAACAGCCTCCTCATCATAAGGCAAGTTAGGCGGGATTA TTTGAAAGGGGCTCTGGCAACTCTGAGAGCAGCCTTGGATTTGAAGCAGGTTCCGTCCCAGATCGCCGTG ACCCGCCTCATCCAGGCGTTGGCCTTGAAGGGTGATGTGGAAAGCATAGAGGCCATTCAGAGAATGGTGG CTGGACTTGACACGATTGGACTCTCAAAAATGGTTTTTATCAATAACATCGCTTTGGCCCAGATGAAGAA TAATAAACTTGATGCTGCCATAGAAAACATTGAGCACCTGCTTGCTTCCGAGAACCAAGCCATAGAACCT CAGTACTTTGGCTTGTCGTATCTATTCAGAAAAGTGATCGAAGAGCAGATGGAACCAGCGCTAGAGAAGT TAAGCATCATGTCTGAGAGAATGGCGAATCAGTTTGCACTTTACAAGCCCGTCACTGATCTATTCCTGCA GCTTGTGGATTCAGGCAAGGTGGATGAGGCCAGAGCTCTCTTAGAGAGATGCGGTGCCATTGCCGAGCAG AGCTCGCTTCTGTCGGTGTTCTGTCTGAGGACTTCTCAGAAACCGAAAAAGGCACCAGTTCTGAAGACTT TGTTAGAACTGATTCCTGAGTTACGTGATAACGATAAAGTATATTCTTGCAGCATGAAAAGCTATGCCTT AGACAAAGATGTGGCCTCGGCTAAAGCACTGTATGAGTATTTGACAGCCAAGAACTTGAAGCTAGATGAC CTGTTTCTCAAGCGCTATGCAGCTTTGCTCAAGGATGTCGGCGAACCAGTCCCCTTCCCCGAGCCCCCTG AAAGCTTTGCATTTTATATAAAGCAACTAAAGGAAGCAAGGGAAAGCCCTTCATGAGAGAAGCAGCGCGG CTGTGTGTGTGTCTATGTGTGTGTGTGTCTGTGTGTGTGTCTGTGTGTGTGTCTGTGTGTGTGTGTCTAT GTGTGTATATGCGCGCGCACATGCCTATGTCTAAATGTTATTTCTAAAATGTACTTGAGAGGAAAATAAA CCAATGAAAATGTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6417407 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6417407 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7672 same embryo
	EMAGE:7674 same embryo
	EMAGE:7675 same embryo
	EMAGE:7673 same embryo
	EMAGE:7676 same embryo
	EurExpress:euxassay_005284 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source