EMAGE mouse gene expression spatial database

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EMAGE:7674

Fam53c family with sequence similarity 53, member C ( MGI:1913556)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005270. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005270_01	euxassay_005270_02	euxassay_005270_03	euxassay_005270_04

euxassay_005270_05	euxassay_005270_06	euxassay_005270_07	euxassay_005270_08	euxassay_005270_09

euxassay_005270_10	euxassay_005270_11	euxassay_005270_12	euxassay_005270_13	euxassay_005270_14

euxassay_005270_15	euxassay_005270_16	euxassay_005270_17	euxassay_005270_18	euxassay_005270_19

euxassay_005270_20	euxassay_005270_21	euxassay_005270_22	euxassay_005270_23	euxassay_005270_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7674_wholemount_strong.wlz

7674_wholemount_moderate.wlz

7674_wholemount_weak.wlz

7674_wholemount_possible.wlz

7674_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7674_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8916
Entity Detected:	Fam53c, family with sequence similarity 53, member C ( MGI:1913556)
Sequence:	sense strand is shown >T8916 GGTGAGAACAAATTACAGAGCAAGGGGACACAGCTCCGCAACCGTTATAAATCTCAGGTTTATAAAAGCT CTAGCAGATCTGGCAGCTAGAAGAGGGGCTCCGGCTACTCAGAGCAGGCCTTGACCGGCGCTTGGACTAC ATTTCCCAGCAGCCTTCACGCCGAGGATCAGTCCTTGCCGGGGGAAGGTGGGGGGATGCTTGCAGAAAGG TGGCTGTGTCGGAAGGGAAAGGAGGCCAGGCACAGCAGGCATGACTGGAGAGGGAGGTCCCAATGGTGCT AGAACGGTGCTGCAGTGGCAGAAGGAGGCTCATGAGTGAGATCCAGCAGAACGACAAGGAAGACATCCAC CAATCGCTCCAAAGTGTGCAAGCCAAGTCCTGGGCAGAAACATGATCACCCTGATCACGGAGCAGCTCCA GAAGCAGACCCTGGATGAGCTGAAATGCACGCGCTTCAGCGTCAGTCTGCCTTTGCCTGATCATGCAGAC ATACCCAACTGTGGGGACCCTTTCCAGCTTGTGTCTGAAGGTGCTTCCTGGAGGGGCCTGCCCCACTGTT CCTGTGCTGAGTTCCAGGACAGCCTCAACTTCAGCTACCATCCCTCAGGCCTAAGCCTGCACCTCAGACC ACCCAGTCGGGGAAACTCCCCCAAGGAGCCGCCCCTCTCCCAAGTACTGAGCCCCGAACCCCCAGACCCA GAGAAGCTTCCTGTGCCCCCTGCCCCTCCATCCAAGAGGCACTGCCGCTCACTCTCGGTGCCTGTGGACC TGTCTCGCTGGCAGCCGGTGTGGCGGCCTGCCCCCTCCAAGCTGTGGACTCCCATCAAGCACCGGGGCAA TGCTGGAGGGGGTGGGCCGCAGGTGCCTCAGCAGAGCCCTCCGAAGCGGGTCTCCAGCCTCAGGTTCCTC CAAGCTCCCAGTGCCTCGTCTCAGTGTGCCCCAGCCCACAGACCCTACAGCCCTCCTTTCTTCAGCCTGG CCCTGGCCCAAGATTCCGCTCAGCCCTGCGCCACCTCCCCTCAGAGTGGCTCCTGGGAGAGTGATGCAGA GTCCCTGTCGCCCTGCCCACCCCAGCGCCGCTTCTCCCTGTCACCCAGCCTGGGCCCACAGGCGAGCCGC TTCCTGCCCTCTGCCCGGAGCTCTCCCGCGTCTTCCCCAGAGCTGCCCTGGAGACCCCGAGGTCTCCGAA ACCTTCCCCGAAGCCGCTCACAGCCTTGTGATCTGGATGCCCGCAAAACTGGTGTCAAGCGGCGTCATGA GGAAGACTGCAGGCGCCTTCGGCCTTCCTTGGACTTTGACAAGATGAATCAGAAACCATACTCAGGAGGT CTCTGTCTCCAAGAAACAGCCCGAGAAGAAGGCAGCAACGTCTCTCCACCATGGTTCATGGCCTGTAGTC CCCCACCTCTCTCTGCTTCCTGCAGTCCCGTCGAGGGTTCATCCCAGGTGCTGAGTGAAAGCGAAGAGGA GGAAGAGGGGTCTGTGCGGTGGGAACGGCAGGCACTGAGCAAGCGGACATTGTGCCAGCAGGACTTTGGG GACCTGGACCTGAATCTGATTGAAGAAAACTAAAAGTGAGAGGCTGCTTCCTGGGCCCATGCAGACTGTG TAATGGTTACTAACACGTGTGAGGGCCCCAAGGCTGAGGGCCCAGCCTGGGAAAGGGGGTGAATGGAGGG CTCCTACTCAGGGCAGCCCAAGTCTTCCTGCTCCAGCAAACTCGACCATGCCAAGAGACTGGCGGGGACA AGATAAATGGAGCTGGTGACGGGAGGGACAGCCCCAGAGCAGACCCTCCCCATGGCGGCCCTGAGTGTGA GTATCCCTGCCACTAAGAGCAATGGGCAGGGAAGGAAGGGGTCTGCGACCCCAGCTCGGGGAATTTCACT AGCCCCTTTGCTTCAAAGGGCACTTGTGTCTTAGAATTTGGCCAGGGTGGGGGGGTTCAGTCAGCCTCCT CGGAGAAATTGTGTGAGTGTGCGTGCGTGCACGGGTGTGTACTTGTGTGAGTGTGTGTGCGTGCATGGGT GTGTACTTGTGTGAGTGTGTGTGCGTGCGCGCATGGGTGTGTACTTGTGTGAGTGTGTGTGTGTGTGTGC ATGGGTGTGTACTTGTGTGAGTGTGCGTGTGTGCACGGGTGTGTACTTGAGGAAAGGTGCGTGCGTTTGT ATAGCCTTAGGGAAGGAAGGCAGTTGCTCCTTTAACAGCAGAGCGTCAGGATACCCCCAGGATCTTGTGG GTTTTTTTGTTTGTTTGTTTCCTTAATATAACATTGGGCTTGTTCAAGGTGTCAAGAGGAGAGAGTTTTT GTTTTTGTTTTTGTTTTTTTTTTCGGTCTTAATAGCTCTGGAGCCAGGCTTTTAAGTTCATCGCATCACT GCTCTGTAAATGGTTCTGTTTTGGGGTCTAGCAGAATCTTTTGAGTACTATGATTTCAGCTAATGGGCTT TTCTGAAGTCCCGCCCTCTCCCACTAGATGAATCTAGTATTGAGGCTTGAGGCAGTCCCCACACTTGTTG TTTTCATTGTCTAAGCTGTCTGCAGCCCGCTTCTCTCGCTTGCTGAGAAGCTGCAAGCAGATGGACAACT GAGGCAGAGCTAAGGAGCTTGGGCGCTCTCCTCTTCCCCAAGGGGACCTTTCCTTTTCTGATCTTACAAG TCAGAGACAGGGTTGCTCTCCAAGAATGTGTTGAGGCAGACCAGGAGCAGTGACATTATTCCCTGGAAGT CCTTGAGGCCACCCTCAGCACTGCCTTTTGGAAGCTGGCTGTAGGGAAACTTACTCTTGCAATATCTAGG CCCGGTCTCTCTCTGATCTGCTTGAAAAAGAGGTAGGGTGTTCAGCATTTAATAGTTTGCTTGTCTTCCA CACTTGCCAGGAAATGTTTATTTGCTTCTAATTGGCCCTGAGAGACCACAGAGAGGCTGGGGCTCACCGA GGGATTGACTTGGGGTGTGTAAAGCAAAGGCTATGAGAAGCAGCTGGGAGTTTGTTGTTGTTGGATTGAA TTTCTTTTTTTGTTTCTCACCCACAACTTCAGCCAGTGCAGCAGATCCAGTGGTTGGAGGGATGGACTTG AAGATTGCAGTTACCTTGGTAGGGTTGGCACCTGTGGGCATGCATATAGAAGCTGTCACTATGCATCCTG TGAGGCTTTGTTTAGAACACCCTGTATAGCGAAATTGGACCTGCCCTTCCCAGTTGTTATAGTAAGGGCA CTTTTCTGTTCAATTTCCATGGTGTCTTAAGGCCCAGTTCCTAGTGTTACAGAAGACAACTAAGGTCATG TACTTTCTTCCTTGTGGAATTGAGCCTGATGTTTAATGTCTCCCACATGCTAATAGGAATACTATAGAGG CGTCAGACTCTTTGCCTCCACTTCAGGGGAGGTGGCCATGTCTTCTTAAACCTGTGGCCCTCAGAGGTTC TACCTTCTCAAGGCTCCTCCCTGTCTCCTGGCTAGTTACCACATCTTACCACTGGGCTACCGGTCTCTAA GGGCCTACGAGAGACTCTCCTGAGAATTCAGGGAAACTGAGCCCAGGAGTTATCGTAGGTTTCATCTTTC CCTGGTTCTCCCTCTTTGGTCCATAATTTCCCTGGTGCCAGCTTCTTTTCCACCTGGGGCAACCTCATCC CTGGGCTCTCTGCTGCAGATGGCAGTGTCAGCCTCCAGTAGGTCACTGGCAGTGCCAATGGGGTCTCTGC GGGCCCCAAGTTTAAGTGAAGGCAGTGATTCTGCCTCCAACCTGGCTTTCCCAAAGCCCTTAGGTCACCG CCCTTAGGGAAGGAGGCCTACAATTGTCATATTTTAATTCCATTACTTTAAGTCTGGAATGTTAAACTAG GCAGAACTATCATCTTGCTTCCCAGTTTAAATGCCTCTGGCCCTGGAACAAGCCTTTTCAAGACGTGAAT CGTTGGTTTTACACAGTAGCTCTGCTCCAACGGTTCTGTGTAAACCAAACGCTGTTTTTTAATGCATTTG GACTGCTGACCATGTAAAGGCAGCCTATGTTTGCTTCTTTTGTAAAGCAAGCAACTTCCCTCTACTTTAG CCAGTTTCATCATTTGGGTTCTCACACAGTGGGCTTACCCAGAGTGGAGCACACCTCCGGGGCTTGTGGC TGCTGCTGTGTAGACGCCTGCATCTGTGCTGCGTGTGTGGTGCGTGTGGCTGGCCAGCAGGTGAGTCTTA CTGCGTGCGTGAGTGAAGCCACTCCCAGGCTGATGCTCTCCTCCTGTGCCCGGTGACTGCCCAGTGGCAG CTCTAGCTGCCCTTCATTCCCACCTGCTGCCAAAGTCCCTGTGCTAATGGGATTACAGAAACAAAAAGTG GAAAAAAATTTTTCGTAAACTTTGTTTTATATTAAAAAATCCATAAGTCTGTGTGTGTTAAAACATGAGG TTCTGCCTCTGTGGCTGTGTTTGAAAAAATAAAGTTTTATTAGAATAATCCATTTTCCCAAGCAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6417613 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6417613 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7672 same embryo
	EMAGE:7675 same embryo
	EMAGE:7677 same embryo
	EMAGE:7673 same embryo
	EMAGE:7676 same embryo
	EurExpress:euxassay_005270 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source