EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7707

Foxj3 forkhead box J3 ( MGI:2443432)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007440_01

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7707_wholemount_moderate.wlz

7707_wholemount_weak.wlz

7707_wholemount_possible.wlz

7707_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7707_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7789
Entity Detected:	Foxj3, forkhead box J3 ( MGI:2443432)
Sequence:	sense strand is shown >T7789 GGCTGCTCCCGCCGCCGCTGCCGCCGCCGCCGCCACTCTCGGCGCCGCTCCGGGCCTGGGCCGCTCTCTG AGGGCGCCGTTGGGACCTTTCGCGGGGAACGGAGACGCACGCAGCGGCCGGAGGCTCGGCCGGGCCGTCC CGGCCTGCGTAGCCTGAGATTCTCTTTGTGGCCAGATGGGTTTGTATGGACAAGCTTGTCCATCTGTAAC TTCATTAAGGATGACATCTGAACTGGAGAGCAGCCTAACATCTATGGACTGGTTACCCCAGCTCACCATG AGAGCAGCCATCCAGAAATCTGATGCTACACAAAATGCACATGGAACGGGAATTTCTAAGAAGAATGCAC TCCTTGACCCAAATACAACACTGGACCAGGAAGAAGTCCAACAGCACAAAGATGGAAAACCTCCGTACAG CTATGCCAGCCTCATCACATTTGCAATTAATAGCTCACCTAAAAAGAAGATGACATTAAGTGAGATTTAC CAATGGATTTGTGATAACTTTCCGTATTATAGAGAAGCTGGCAGTGGTTGGAAGAATTCTATACGACATA ATCTGTCACTGAACAAATGTTTCCTTAAAGTGCCTCGGTCCAAGGATGATCCCGGAAAGGGTTCTTACTG GGCAATAGACACCAATCCGAAGGAAGACACGCTGCCTACTCGGCCAAAGAAGAGGGCAAGATCCGTAGAA CGGGCCTCAACTCCATATAGCATAGATTCAGATTCTTTGGGAATGGAGTGTATTATTTCGGGAAGTGCCT CTCCAACTCTGGCAATCAACACTGTGACTAACAAAGTAACATTGTACAACGCTGACCAGGATGGTAGTGA TAGCCCTCGAAGCAGCCTTAACAACAGTCTCTCAGACCAGAGTTTGGCGTCTGTTAATTTGAACAGTGTT GGAAGTGTACATAGTTATACACCGGTCACTAACCATCCAGAACCAGTCTCTCAGCCATTAACACCTCAGC AGCAGCAGCAGCCACAGTACAACCTTCCAGAACGAGAAAAACAACTCCTTTTCACAGAATACAATTTTGA AGATCTCAGTGCCTCATTTCGGAGTCTTTATAAGTCAGTTTTTGAGCAGTCACTTAGTCAACAAGGTTTA ATGAGCATCCCTTCGGAATCTTCCCAACAGTCCCACACTTCATGTTCCTATCAACACTCTCCTAGCAGTA CAGTGACCTCTCACCCACACAGCAACCAAAGCAGCCTGCCCAACAATCACAGTGGCCTCAGTGCCACAGG TAGTAATTCGGTTGCACAGGTCTCGCTGTCCCACCCCCAGATGCACCCCCAGCCCTCTCCACATACACCC CATAGACCGCATGGTTTACCCCAGCACCCACAGCGTCCCCAGCACCCAGCACCACACCCACAGCAACACA GCCAGCTACAGCCGCCTCACTCCCAGCACCCCCCTCCACATCAGCACATACAACACCATCCGAACCATCA GCATCAGACATTAGCACATCAGCCACCGCCACCCCCGCAACAGGTATCCTGTAATTCTGGTGTTTCAAGT GATTGGTATGCAACACTCGATATGCTGAAGGAAAGCTGTCGAATTGCCAGCAGTGTTAACTGGTCAGATG TAGACCTTTCACAGTTCCAAGGTCTGATGGAGAGTATGAGGCAGGCGGATCTCAAGAACTGGTCATTAGA TCAGGTTCAGTTCGCTGATCTCTGTTCCTCTCTTAATCAGTTCTTCACACAGACTGGCCTTATCCACTCG CAGAGTAATGTTCCGCAGAATGTCTGTCACGGTGCCATGCACCCAGCAAAGCCTTCCCAACACATCGGGG CAGGAAATTTGTACATAGATTCCAGGCAAAGTCTCCCTCCCTCAGTGATGCCACCCCCTGGTTATCCTCA TATTCCACAGGCGCTCAACACTCCAGGAACTACGATGGCAGGTCATCACGGAGCCATGAACCAGCAGCAC ATGATGCCTTCCCAAGCCTTCCCGATGCGGCGCCCTCTGCCTCCAGATGACATCCAGGATGACTTTGATT GGGATTCAATAGTGTAGGCTTGTTTCTGCAAGACAGCAGACTCCAGGATTCTTTTTGTTCAGTGAAAGGA CGGATTAGAGGCCGGTTGAGAAAACAAAGCTATGTTACCAGTGCTATCTAAATTCCTTTCGAAGACAATC AGAGATTCTAGCTGGGTAACTTACTGCTTTTATCCAACCCAAGCAAGAACTACATGTTTGTCTCCCTGCC AGTACCCTCTGTAGCTCCTAACTGTTGTGATTTGGACAGCTTTTTGCATATTTGTGTCAGTTTGATGTTG ACCACAAGTGCCAGACTGATTTTTCAGACAGAGCCTATTTTGCTGCGAGCAGTTTATAGATACATATGTG TAAATATGTGTACAAATATTACTGAAAGGCTTCAAGTTTTTTCTAATTGGATTATTGTATCTTGAAAAGA AAGTTACTGTCAGTTTTTATTCCTTGTTAGGCCATTTTCTGCAGGATGCTTTCAAATGATACAGAGAGCT GAAAGGAAATCAGTTTTTCAAAGCCCAGTTTCCTTTATTTAATCTTCAGAAATAGGAGTAATCATGAATC CACCGAGTAAGTCAAAGAATCTGGAGTTGGTACTCAAAAACAGTGCAGAGGGCCGTTGTTCCTGAGCAAG CTTGTCACTTGTCATTCGGTGTTGTAGAGAGAGTGTTGTACCTGTCAGTGTGATTCCTCAGCTGCATCTT GGCAGCTCTGAAATGTGTGGGCTGACTGAAACTGGGTATTGCTTGGCTCTTCAAACAAACCAATGTCTCC ATAGTCACTTATGTATTTATTATTCAAAAGTGTTATTTTAATATTTATTGCTCTGTGAATGCACAGTTCT GTTGGATTCACTAGGGAGGGATTTGTCTGAAAGCATGGTTATTTTTCTGGATAAAGTTTACAGACAAAGA CAATCAGAAGCATTGGTCCTTCTCTTTTATCATCACTCCTTACCAGGTGAGCGTGCTTCCTGTCTTGCTT AGCCTTCCTGCTGGGGGAGACTTAATAGCTACATAGGAATTTCCTGGAATTTGAATCAGTCTATTTAGGC AGCAAGATACAGAGGCTGGGTTTATACAGCAGTGTCCCTGCTGTTGGTAACGCTAGCAGGGGCTGCATGC AGCTCCAGAGTCTTATCTGCTTCAGTCTTGAGTGCAGTGACTGACTGCGTGACCTGCTGTTGGGTCTGTG TGTCCCCAACATGAAGCACCTCAGAGCTTTGATCAGTTGCTTTGTTTTGTGTCATGTCACAGCTAGCATG GGTTGAATGTGTTGATTTTATAATGAAAGTAAAGAAAGGAGATTCTCCTCCCCACAGTGAATAGATCGTT AATTGGTTCTTTACAGAGAGGGAAATCCATTTTAATGAGAAAATAGATTCTGTCAAATCAAGAAGAAATA ACTGGTCACCAGTTGCTTGTTAGTGTCTCTGGGCTAAGCAGTGCTTAATCACATTGACTAACCTCAGCGA GTCTGTGGATGAGTAATCTCTACTGGAACAAAGGTTTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG TGTGTGTGTGTGTGTGTAAGTGCAAGAGATCATGAAAGAGACGGGGCACGGGGAGAGAATGAGAATATCT GTGTATGTGTTAATGGAGTGTTGTCTTGAATGAATCCCTGGGAAGCCATGGGGAGTGCAGGGAAGAAGGG AGAGCCTCGGGCTTCGCTGTTGCTTGGACCCCCACTTGGCATGAGAGAAAGAAGCTCAGGTACAGAGGAT TCTCAACCAATAGCCAACAGGCCTCACTCGGAAGGACTGGGCAAGATGAGATGGCTGTCTGCAGAGAAAA AGCTTGGGAAAATGAGGTCCTCAGTAGTTTGCTCAAGTCTCTGTTTTCTAGACACCAAAATGGCTGCTCT GTGACTAGGATTGCTCCGCAGCAGTGTGCACTTTAAATGATTGGGTGTTGGAGTATCGCAGTCTGAAACA GCATGGCTTGATGAATTTACAGATTATGTGAAGCTAACTTCCCATTTGACAATTACTGATTTGCAGTGAT CGATATATTTATGAATGTTTAAACTGACCAAGAATGGCACTTGAGGCCACTCACCACTCTTCTGTGATTC CCTGTTTCCCTGGGGCTCAGCAAGCCTGAGTGCCTGGGCAGGGAAGGCTACCTGGTGGGCGCAGAGTCCC TCTAGTGGCCATTTCTGATTTATTTGATGACACACAGCAAGTTCTCTAGGGCTGAAATTAACTGGAGACT TGGGGTAAGGACTAACAAACATACATACACAGATGGGGCGCTCTGTCCAGGAGAAGAGTCCGACTGGCAT TTGTGGCAGTGTTTGAAATGTAAATGTACTCCTGTGTGTTCCTGTAAATATTTAATATGTGTCTCAGACG TCCTTTGAAGTGGGAGGGGATCGATCTTGGGATAATTTCAAATGGAATAGAGTATTTTGATATGTTCATT GAAAGGGTGATGTGTACATACTTATATTGTATATATGTGGTGACATGCATTGGCTTTTGTGCAGAAACAA CCTGCTCTTGTGCTGCTGTTGGACAGTCAAGTGTTTTAATGTTTCTACAGTTTTCCTATTGCACGATTTC ATATTCTGCCAATGGTGAGTGGCACCCATTCTTTGTAACCGTATAGTGCTGTAAAGAAATATTCCAAGTG TCATTAGGATTGTCGCTGCCAGAATTGATATGCATGGATGGCACTTAAAATAAATATATTATGATAACTG TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6314981 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6314981 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7708 same embryo
	EMAGE:7710 same embryo
	EMAGE:7709 same embryo
	EMAGE:7706 same embryo
	EurExpress:euxassay_007440 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source