EMAGE mouse gene expression spatial database

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EMAGE:7714

Arhgap1 Rho GTPase activating protein 1 ( MGI:2445003)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007458. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007458_01	euxassay_007458_02	euxassay_007458_03	euxassay_007458_04

euxassay_007458_05	euxassay_007458_06	euxassay_007458_07	euxassay_007458_08	euxassay_007458_09

euxassay_007458_10	euxassay_007458_11	euxassay_007458_12	euxassay_007458_13	euxassay_007458_14

euxassay_007458_15	euxassay_007458_16	euxassay_007458_17	euxassay_007458_18	euxassay_007458_19

euxassay_007458_20	euxassay_007458_21	euxassay_007458_22	euxassay_007458_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7714_wholemount_strong.wlz

7714_wholemount_moderate.wlz

7714_wholemount_weak.wlz

7714_wholemount_possible.wlz

7714_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7714_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8969
Entity Detected:	Arhgap1, Rho GTPase activating protein 1 ( MGI:2445003)
Sequence:	sense strand is shown >T8969 GTTTGCCGAGCTTTGACAGGCGTGGCAGAGGAAGCATTGCCGGAACGAGCCATGGACCCTCTCTCAGAGC TGCAAGATGACCTGACGCTGGATGACACCAGCCAAGCTCTGAACCAGTTGAAGCTGGCCTCCATTGATGA GAAGAACTGGCCTTCTGATGAAATGCCAGACTTCCCCAAGTCAGATGACTCCAAAAGCAGCTCTCCAGAA CCTGTGACACACCTGAAGTGGGATGACCCTTACTATGACATTGCCCGGCACCAGATTGTGGAAGTAGCCG GTGATGATAAGTATGGGCGGAAGATCATTGTATTCAGTGCTTGCCGGATGCCCCCCAGCCACCAGCTGGA CCACAGCAAGCTCTTGGGGTATTTGAAACACACATTGGACCAGTATGTGGAGAGTGACTACACACTCCTG TACCTGCACCATGGCCTGACCAGTGACAACAAGCCCTCCCTCAGCTGGCTACGGGATGCCTACCGAGAGT TTGACCGCAAGTACAAGAAGAACATCAAGGCTCTGTACATCGTGCACCCAACCATGTTCATCAAAACTCT GCTTATCCTCTTTAAGCCTCTCATCAGTTTCAAGTTTGGACGGAAGATCTTCTACGTGAATTACCTGAGT GAGCTGAGCGAGCACGTGAAGCTGGAGCAGTTGGGGATCCCTCGCCAAGTGCTCAAGTATGATGACTTCC TCAAATCCACACAGAAAAGCCCTGCAACAGCTCCCAAGCCCATGCCACCACGGCCCCCCCTGCCCAATCA GCAGTTTGGGGTCTCCTTACAGCACCTCCAGGAGAAAAGCCCAGGCCAGGACCCCATTCCCATTGTCCTC AGGGAGACCGTCGCCTACTTGCAGGCTCACGCTCTCACCACTGAGGGCATCTTCCGGAGGTCTGCCAACA CCCAGGTTGTCCGGGAAGTGCAGCAGAAATACAATATGGGGCTGCCGGTGGACTTTGACCAGTACAACGA GCTGCACTTGCCCGCTGTCATCCTCAAGACATTCCTTCGGGAGCTTCCTGAACCCTTGCTAACCTTTGAC CTGTACCCCCATGTTGTGGGCTTCCTCAATATTGATGAAAGCCAGAGAGTGGAAGTGACACAGCAGGTTC TCCAAACTCTGCCAGAGGAGAACTACCAGGTGCTTCATTTCCTGACTGCCTTCCTGGTGCAGATATCTGC CCACTGTGACCAGAACAAGATGACCAACACTAACTTGGCTGTAGTCTTCGGCCCTAACCTGCTCTGGGCT AAAGATGCTGCCATCACCCTCAAGGCTATCAATCCCATCAACACCTTCACTAAGTTCCTCCTGGATCATC AAGGGGAATTGTTCCCCAGCACAGATGCCCAGGGGGTCTGAGCTCACCACCTCCAGCCCCTTCTCTGGCA GCCCGAGTTGGACTTGTCCTCCTGGCATTAACCTTACTGGAGTCCAGGGCTCCTGCCTAGGAAGGGGCCC TGAAGCCCCAGAAGATGGAAGTTGGAGCCTACTAACTGGGCAGCCCCTCCTGTCCCTCTGCCCGTCCCAC CTTACCGGCCCTGGAGGCCTCTGTTACCGTAAGACATTTTCTTTGCCTTATACCTCTCACTGCCTCTCTG GTTGCTGGGCTTGGTTCTTCTAGGATTTAGCCACAGTAAACCTTTTCCTTTTTCCTGCCTGTTTCCCTGG CGAGTGTAGAAGACTACATCACTGCTGGCTGTGCTGGTAGCTGGGGCTGGTCCTCTCTTTCCTGATTGCT AGGAAGTGAGCATTGGCCTGGGTGCAAGTTCCCTGTGCCTGCCCTCCCTGCTAATAGGGATGATAAGACC AGAGTTGGCCTGTCACTTCTGCCTCCTGGGTCTGTTCTTCCCCAAGTGGTACTGCCCCCAGAGTGTACTA TTCCCTCATTTCTGCCACACTTGGCACTTTGGATATCAAGACTCAGAATCTGAGTGTCTATTGAAATGGT CCTGTCCACTCCCAGGGAGAGCCGTAGTGTCTGGTAGGATCAGTTACAGCAGTCCTGGCCCTCCATTCAT TCTCATGTGCACAGATCTTCAGCCAAAGGCTATAGTAGGTCTTTCAGGTGTGGAGTCATTTTGATCCCTG TTCTAGCCAGAGATACCAATGTCTCTCACTTTCTACCTGGGGACTCTCGGAGAGTCAGAAACTCAGAAGC CCTGAAGACTGACAGAGCTTTTACTAATAGATCACCCCTGCTGGATAGGTTCTAGCAGCTTCCTTAGGGG AGTGTGGTGGCCACAGGGCCTCTTGTCTTTCAGAAAGCTGAGCCCCAGTGATGATTGGTAGCTTTCATTC TATTCCTAATGAAGCCTTGTGACTCTTCCCGAAGTCCACTGCCTAATGACAGGGAGGTACCTGCAGTGAC CTTGCCCTACAGGTGTGGCTAGGCATGACAGCTCCAGAGGTGGTACTGGCTACCATGAGATCTGTACTGA TGATCTTGCAGCTGAGGCTGGGCCTGACCTCACTGGCTCTTTCTTGCACCACTGTTGTAGCAGGTCTCTG GTGCCACAAGTGCTGCTGTCAGTCCCCAGCCTCACACCTGACCCAGCCTGGGTCACTCTGCTTTTGCCCA CATTTTTGTATCAAGTGTTGCTACACTCCATTTACTGAGGAGCCTTTGGACCTCTTGTTTTCCTCCTGCC CGGTCCTGTCAGTCTGTGGCAGGGGACGCTCTTACTGTGAATCTAGTGTTCACATTTACCACGATTTCCT TGGCACCAATCATGTATTTTACCGTTTGCACTTTTTGTATTTCAATAAAAATGTAAATGAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6810195 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6810195 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7713 same embryo
	EMAGE:7712 same embryo
	EMAGE:7715 same embryo
	EMAGE:7716 same embryo
	EMAGE:7711 same embryo
	EurExpress:euxassay_007458 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source