EMAGE mouse gene expression spatial database

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EMAGE:7715

Lrp11 low density lipoprotein receptor-related protein 11 ( MGI:2442989)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007470. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007470_01	euxassay_007470_02	euxassay_007470_03	euxassay_007470_04

euxassay_007470_05	euxassay_007470_06	euxassay_007470_07	euxassay_007470_08	euxassay_007470_09

euxassay_007470_10	euxassay_007470_11	euxassay_007470_12	euxassay_007470_13	euxassay_007470_14

euxassay_007470_15	euxassay_007470_16	euxassay_007470_17	euxassay_007470_18	euxassay_007470_19

euxassay_007470_20	euxassay_007470_21	euxassay_007470_22	euxassay_007470_23	euxassay_007470_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7715_wholemount_strong.wlz

7715_wholemount_moderate.wlz

7715_wholemount_weak.wlz

7715_wholemount_possible.wlz

7715_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7715_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 09 10 16 17

pons mantle layer

moderate

regional

moderate expression: see section 07 17

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 19 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 09 18

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 17 18 19 20

trigeminal v nerve

strong

regional

strong expression: see section 09 10 16 17

ventral grey horn

strong

regional

strong expression: see section 09 10 11 12 13 14 15

cervico-thoracic ganglion

strong

regional

strong expression: see section 09 moderate expression: see section 10 16 17

cervical ganglion

strong

regional

strong expression: see section 09 moderate expression: see section 10 17

thoracic ganglion

moderate

regional

moderate expression: see section 11 12

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 13 14 15 16 17 18 19

neural retina

moderate

regional

moderate expression: see section 01 02 03 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 14 15 16 17 18

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09

midgut

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

lower jaw incisor

strong

regional

strong expression: see section 11 12 13

upper jaw incisor

strong

regional

strong expression: see section 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8975
Entity Detected:	Lrp11, low density lipoprotein receptor-related protein 11 ( MGI:2442989)
Sequence:	sense strand is shown >T8975 AGCCGGCACACTCGCCGCCTCCGTGAAGCAGCCAGGCCCCGGGGCCGCCGAGCTCTGAGGCCGCGCTCGT GCCATGGCCACAAGGGGAGGAGGACCGGGCCCCGGCTTCCGGCACCGCGCGCTCCGCGGGCTGCTGCTGC TCTGCCTCTGGCTGCCCGGCAGCCGGCCCGGGGAGCCCGCCGCTCCGTCGTCCGGAGTGGATAGGCTGCT TCAGGACTTTCGCCGGCAGCTACAGCGGGCACGGCCTCGCGAGGAGCTAGAGCCGGAGCTACTGGGCGGC CCGCGGGAGGATTGCCCGGGTGCGGGCGGTACGGCGGTCTACCGGGCGGTGCCCGATACCATCATCCGCA CCCAGGATTCCATCGCGGCGGGAGCCAGCTTCCTGCGCGCACCCGGGAGCGTGCGGGGTTGGCGGCAGTG CGTGACGGCCTGCTGCTCCGAGCCGAGCTGCTCGGTGGCAGTGGTGCAGCTGCCCCGGGGGCCCAGCGTG CCCGCACCTATGCCCGCACCCCGCTGCTACCTGTTCAACTGCACGGCGCGCGGCCGCAGCGTCTGCAAGT TCGCGCCACTCCGCGGGTACCGCACTTACACCCTCAGTCGCGCCGAGGACGCCGCTGGGATCCCTCCCCG GCCAGATGAGGACAAGCCTCCCGTTAGCAAGGCTGGGAAGGATGTGGTTTTACATCTGCCCACAGACGGG GTAGTTCTAGATGGTCGAGAAAGCTCGGACGACCACGCCATTGTCCTTTATGAGTGGACGCTGCAGCAGG GTGACCCATCGTCAGTGGACATGAAGGTGCCTCAGCCAGGAACCCTGAGGCTGTCCCGCCTGAAGGAAGG AGCTTACATCTTCCAGCTGACTGTGACTGACTCCGTGGGTCAGAGAAGCTCCGATAACGTCTCTGTCACT GTGCTTCCCAGGCCCTACTCCACGGGAGGATGCTCCAGTGCTTGCTCGCGCTACCATTTCTTCTGTGACA GTGGCTGCTGTATTGACATAGCGTTGGCTTGTGATGGCGTGAGGCAGTGTCCCGACGGGTCTGATGAAGA CTTCTGTCAAAACTTGGCTCTTGACCGCAAGTTGGTGACACACACAGTGGCTACTTCTGCCCAGCCAGGA GCCATGGGATTAAATGAAGGTGAAGGAGACCCCAAGTTGGAAAAATCCCAGAGAGCCACTACCCACAACC AACCCGCTACTGTATCCCACCCAGAGACGAGGATCCACTCCACCCAGAAGGCGCCAGAGAGTCAGATCAA TCCTGTCCAGCCAGCAATTCCTCAGGAAAGAACCAAGAAGAAGGAAATTATGATCTCAAGTCCAAGAGTG GCCAAGCAGGAGGGGAGCACCCGGCCCCGGAAGCAGGTGCGGTGCTACCTCTGGCATTAGGTCTGGCCAT CACTGTTTTGTTGCTTCTCATGGTGACATGCCGACTCCGACTGGTGAAGCAGAAACTCAAGAAAGCCCGG CCCATTACATCTGAGGAATCGGACTACCTCATCAATGGGATGTATCTGTAACAACGCTATTTAAGCAGCT CGGGGCAAGGATGGCTATCACTCACAATCTGTTCTTTGGGTTCTATCATTAAGATGTTTTGTTTTAAACT GTGCTAGAAGACAGTGCAAACACAAGCCTTGACTTATCATGAAATAATTTCCCTGGGATGTACAATGTCT TCAAAGGTAGAGAGGAATTCATAAGACTGAAGCTTGTTTGCTGAGACAGAAAGTCAGCATGTGACAAGGT GACAGATGGAAGTCCTGCCTTTTATTTGTGAGGTGCCAACTGCTGGCCCCTATCAGCTTTCTTATCTTCT GGCTCTCTTCTCTCCACTGAGTTCTCATGAGCTACACTTTCTTTTCCTCTGCTAAATACCTCTGAAAAAG GATCTTAAGTGAATTTACCCTCAGTGAGATGTTACCAATGGCAGGTCTGTCTGGATGTTATAAGCATGCT GATTAGATCAGTGATGTCCCACAGGGGAGGAATATAGGTTCTCCACCCAGCCCCACCCTATGCTTCTGAC CAGGCCAAGGTGGTAGCTGCCACACCTGGCTGACCTTAGAAGAATCCCTTTTGGCTAGGGGCAACTGTAG GTGGTTATGAAATGAGAACAAGCTGGCAGCAGAGGAGAGGGACCTGGCAGGCAAAAGCAAATAGAACCCC AGCCGTGGGATTCGTAAGGACAATCTACTCAATGCTGCTTCATGGAATGGAGCGGTGTGGCTGCTTTACA TGTGCACTGGTTTTCAACATGAGCCTTTCTGATGAAAAAACGTGCGAATTATCAATCCAAAAGGTGATGT CTGGGTGTTTGTAAACATGTTGCCATGTCCAGGTATCACAGGCTTTTCAATGGAATGGGTCCTGATGCTT CCCTTCGTGTGTCTTGCAGGGCTGGAGGTGGGACCCAGGAGCCTGGTTGGGCTAGATAAGTGCTCTACCA CTGATCTACACCCCAGCCCTCACTGTTCTTTATGTTATTAAAATGTTATAATGTACAATCAGCTAATGTC AGGATGTATTTCCAGAGCAGAAAGCACTTTTGCACAAGTTCCCCTTTGCAGGAAACTGGCATCCAGCTTA ACACCTTTAAGTTTGTAGACAGTAAAGGCAGACCTTCCCATCACCACTTAGTGCCAGGTTGTATACTCTA GAGGCTACTCTGAGGGGACTGCAGTGTGGTGCCTGTTTATAATCATGATGTTCCTAGTTCTTTACCCTGA GTGGCAAACGTTACAAAACACTAAAAACCACTGTAGTCTCATTAGTTTAGACTCAATATAAAGTAGGTGG TTCCTTTAACAAGGGGTTTGATTTTGGTGAGGGAATATAACCTATTGAAATGTATTAATCAAAGGGTAGT TCTAGAAAAACAAATGTTTGCTTTTGTCTCTTGAGACTCTAACTCCAGCCTCTTACTGACTATTTAGAGC TAATCAATGGTGTAAATGTTACAGTAGCAGTCTGAGAGATGAATGTTGAGTGAACTTACCTACACTCTGT GATGCTGTGCGGCTCATTTTCATAGAGGTCACCTATAGCCATACCCAGTAAATAGAAACTGATAAGGCAT GTTTCTTTCTGCACATCTTTTATATACAAAGAAGTTATCTACAATCTAGTATTGCTATTGTTTTGAAGTA CTTGAGAAAAGATGCCTTGTATGTACCAGTCCTTTAATAAACTATTTTATTCTTATGCCTTTAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6810402 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6810402 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7714 same embryo
	EMAGE:7713 same embryo
	EMAGE:7712 same embryo
	EMAGE:7716 same embryo
	EMAGE:7711 same embryo
	EurExpress:euxassay_007470 same experiment
	MGI:4825980 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source