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EMAGE:7793

Fancm Fanconi anemia, complementation group M ( MGI:2442306)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7793 EMAGE:7793 EMAGE:7793 EMAGE:7793 EMAGE:7793
"Pseudo-wholemount" of euxassay_005236. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005236_01 euxassay_005236_02 euxassay_005236_03 euxassay_005236_04
EMAGE:7793 EMAGE:7793 EMAGE:7793 EMAGE:7793 EMAGE:7793
euxassay_005236_05 euxassay_005236_06 euxassay_005236_07 euxassay_005236_08 euxassay_005236_09
EMAGE:7793 EMAGE:7793 EMAGE:7793 EMAGE:7793 EMAGE:7793
euxassay_005236_10 euxassay_005236_11 euxassay_005236_12 euxassay_005236_13 euxassay_005236_14
EMAGE:7793 EMAGE:7793 EMAGE:7793 EMAGE:7793 EMAGE:7793
euxassay_005236_15 euxassay_005236_16 euxassay_005236_17 euxassay_005236_18 euxassay_005236_19
EMAGE:7793 EMAGE:7793 EMAGE:7793 EMAGE:7793
euxassay_005236_20 euxassay_005236_21 euxassay_005236_22 euxassay_005236_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7793Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7793_wholemount_strong.wlz
7793_wholemount_moderate.wlz
7793_wholemount_weak.wlz
7793_wholemount_possible.wlz
7793_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7793_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
mandible
moderate moderate
regionalmoderate expression: see section 10 11 12 15 16 17 18 19 20
lung
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
temporal bone petrous part
strong strong
regionalstrong expression: see section 01 02 21 22 23 moderate expression: see section 03 04
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38
Entity Detected:Fancm, Fanconi anemia, complementation group M ( MGI:2442306)
Sequence:sense strand is shown

>T38
TGAAGGGGAGTTTGCCCTCTGCATTAGTTTATATCATGGTTATGAGTTACTGCAGCAAATGGGAATGCGG
TCATTGTATTTCTTTCTTTCTGGAATCATGGATGGAACTAAAGGAATGACACGGGCAAGAAATGAGCTCA
GCCGAAATGAGGACTTCATGAAGCTGTACACTCACCTGCAGAGCGCGTTTGCACCAGCCAGCACCAGCGA
TGCTTCGGCTTTCCAGAGAGGAAATAAAGAGAAAAAATTTGTCTATAGTCATCCAAAGTTAAAGAAACTG
GAAGAAGTTATACTGGAACACTTCAAGTCATGGAATGCTAAAGCCACCACTGAGAAGAAGTGCCACGAGA
GCAGAGTCATGATCTTCTCGTCATTCCGAGATAGCGTTGAGGAGATTGCAGAAATGCTTTTACAGCACCG
GCCAGTTATTAGAGTGATGACCTTTGTTGGCCATGCTTCAGGGAAAAACACAAAAGGTTTTACCCAGAAG
GAACAACTGCAGGTGGTGAGACAGTTCCGAGACGGTGGCTATAACACACTGGTT
Notes:The probe template was PCR amplified from IMAGE:944890 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:944890 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7794 same embryo
 EMAGE:7792 same embryo
 EMAGE:7796 same embryo
 EMAGE:7795 same embryo
 EurExpress:euxassay_005236 same experiment
 MGI:4824776 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS