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EMAGE:7795

Prkcdbp protein kinase C, delta binding protein ( MGI:1923422)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7795 EMAGE:7795 EMAGE:7795 EMAGE:7795 EMAGE:7795
"Pseudo-wholemount" of euxassay_007523. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007523_01 euxassay_007523_02 euxassay_007523_03 euxassay_007523_04
EMAGE:7795 EMAGE:7795 EMAGE:7795 EMAGE:7795 EMAGE:7795
euxassay_007523_05 euxassay_007523_06 euxassay_007523_07 euxassay_007523_08 euxassay_007523_09
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euxassay_007523_10 euxassay_007523_11 euxassay_007523_12 euxassay_007523_13 euxassay_007523_14
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euxassay_007523_15 euxassay_007523_16 euxassay_007523_17 euxassay_007523_18 euxassay_007523_19
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euxassay_007523_20 euxassay_007523_21 euxassay_007523_22 euxassay_007523_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7795Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7795_wholemount_strong.wlz
7795_wholemount_moderate.wlz
7795_wholemount_weak.wlz
7795_wholemount_possible.wlz
7795_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7795_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2159
Entity Detected:Prkcdbp, protein kinase C, delta binding protein ( MGI:1923422)
Sequence:sense strand is shown

>T2159
TGGCCTCGAGCCAGATTCGGACGAGGCTCAGGGAGAGCAGGCCCGGTGGTCAGAGATCATGGGGGAGAGC
GCACTGGAGCCCGGACCTGTGCCTGAGACGCCCGCAGGGGGTCCGGTGCACGCAGTCACCGTGGTGACCT
TGCTGGAGAAATTGGCCACCATGCTGGAGGCTCTGCGGGAGCGGCAAGGCGGCCTGGCTGAGAGGCAGGG
CGGCCTGGCGGGCTCCGTGCGTCGCATACAGAGTGGCCTGGGTGCGCTGAGTCGCAGCCACGACACCACC
AGCAACACACTGACGCAGCTGCTGGCCAAGGCGGAGCGCGTGGGCTCGCACGCCGACGCAGCCCAGGAGC
GGGCCGTGCGTCGCGCTGCTCAGGTGCAGCGGTTGGAGGCCAACCACGGGCTGCTGGTGGCGCGCGGGAA
GCTGCACGTCCTGCTCTTCAAGGAGGAGACTGAAATTCCAGCCCGCGCCTTCCAGAAAGTCCCAGAGCTC
TTGGGTCCGGAGGACCAGTTGGTGCTGGGCCCA
Notes:The probe template was PCR amplified from IMAGE:875998 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:875998 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7794 same embryo
 EMAGE:7793 same embryo
 EMAGE:7792 same embryo
 EMAGE:7796 same embryo
 EurExpress:euxassay_007523 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS