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EMAGE:8862

Tmem180 transmembrane protein 180 ( MGI:1922396)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8862 EMAGE:8862 EMAGE:8862 EMAGE:8862 EMAGE:8862
"Pseudo-wholemount" of euxassay_010032. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010032_01 euxassay_010032_02 euxassay_010032_03 euxassay_010032_04
EMAGE:8862 EMAGE:8862 EMAGE:8862 EMAGE:8862 EMAGE:8862
euxassay_010032_05 euxassay_010032_06 euxassay_010032_07 euxassay_010032_08 euxassay_010032_09
EMAGE:8862 EMAGE:8862 EMAGE:8862 EMAGE:8862 EMAGE:8862
euxassay_010032_10 euxassay_010032_11 euxassay_010032_12 euxassay_010032_13 euxassay_010032_14
EMAGE:8862 EMAGE:8862 EMAGE:8862 EMAGE:8862 EMAGE:8862
euxassay_010032_15 euxassay_010032_16 euxassay_010032_17 euxassay_010032_18 euxassay_010032_19
EMAGE:8862 EMAGE:8862 EMAGE:8862 EMAGE:8862 EMAGE:8862
euxassay_010032_20 euxassay_010032_21 euxassay_010032_22 euxassay_010032_23 euxassay_010032_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8862Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8862_wholemount_strong.wlz
8862_wholemount_moderate.wlz
8862_wholemount_weak.wlz
8862_wholemount_possible.wlz
8862_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8862_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 19 20 21 22 23 24 weak expression: see section 01
mandible
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 17 18 19 20 21 weak expression: see section 04 10 14 15 16 22
maxilla
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 17 18 19 20 weak expression: see section 10 15 16
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 22 23 moderate expression: see section 05 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31820
Entity Detected:Tmem180, transmembrane protein 180 ( MGI:1922396)
Sequence:sense strand is shown

>T31820
GTGCAGCTGGCTCCTTGTCAGTCTTCGCCTCCTACGCCTTTTGGAACAAGGAGGACTTCTCCTCCTTCCG
TGCCTTCTGTGTGGTACTAGCTGCTGGCTCTGGGCTGGGCTTTCTGGGAACCACACAATTATTGAAGCGG
CAGATTGAAGCGACCAGAAGGGACAGGGGTTGCCCGGGCCTGGACCTGGATGGTGGCGTGTGTGAAGAGG
AGCCGCCAGTGGGTGGTGAGGAAGCAGGCAACATCACCTTGGGCCAGTACCTCCGGCAACTGGCACGCCA
CCAAAACTTCCTGTGGTTCGTGGGCATGGACCTGGTACAGGTGTTCCACTGTCACTTCAACAGTAACTTC
TTCCCCCTCTTCCTGGAACACTTGCTGTCTGACCACATCTCCCTGTCCACTGGCTCCTTCCTGTTGGGCA
TCTCCTATGTCGCTCCTCATCTCAACAACCTGTACTTCCTGCCCCTGTGCCGGCGCTGGGGTGTCTATGC
AGTGGTGCGGGGGCTCTTCCTGCTCAAGCTGAGCCTTAGCCTCCTCATGCTGTTGGCTGGCCCAGACCAC
CCTGGCCTGCTTTGCTTCTTCATTGCCAGCAACCGTGTCTTCACTGAGGGTACCTGTAAGCTGCTGACAC
TGGTGGTCACTGACCTGGTGGATGAGGACCTGGTACTGAACCATCGGAAGCAGGCAGCCTCGGCGCTCCT
CTTTGGCATGGTTGCCCTGGTGACCAAGCCTGGCCAGACCTTTGCCCCACTGCTGGGCACCTGGCTTCTC
TGCTTCTACACAGGTCACGACCTTTTTCAGCAATCCCCAATGACCCCTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6399588), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59440. Forward Primer - name:059440_F_IRAV99_f04_4930538D17Rik, sequence:GTGCAGCTGGCTCCTTGT; Reverse Primer - name:059440_R_SP6_IRAV99_f04_4930538D17Rik, sequence:ACAGGGGTCATTGGGGAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8864 same embryo
 EMAGE:8861 same embryo
 EMAGE:8866 same embryo
 EMAGE:8863 same embryo
 EMAGE:8865 same embryo
 EurExpress:euxassay_010032 same experiment
 MGI:4828790 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS