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EMAGE:8882

March11 membrane-associated ring finger (C3HC4) 11 ( MGI:3608327)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8882 EMAGE:8882 EMAGE:8882 EMAGE:8882 EMAGE:8882
"Pseudo-wholemount" of euxassay_010112. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010112_01 euxassay_010112_02 euxassay_010112_03 euxassay_010112_04
EMAGE:8882 EMAGE:8882 EMAGE:8882 EMAGE:8882 EMAGE:8882
euxassay_010112_05 euxassay_010112_06 euxassay_010112_07 euxassay_010112_08 euxassay_010112_09
EMAGE:8882 EMAGE:8882 EMAGE:8882 EMAGE:8882 EMAGE:8882
euxassay_010112_10 euxassay_010112_11 euxassay_010112_12 euxassay_010112_13 euxassay_010112_14
EMAGE:8882 EMAGE:8882 EMAGE:8882 EMAGE:8882 EMAGE:8882
euxassay_010112_15 euxassay_010112_16 euxassay_010112_17 euxassay_010112_18 euxassay_010112_19
EMAGE:8882 EMAGE:8882 EMAGE:8882 EMAGE:8882 EMAGE:8882
euxassay_010112_20 euxassay_010112_21 euxassay_010112_22 euxassay_010112_23 euxassay_010112_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8882Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8882_wholemount_strong.wlz
8882_wholemount_moderate.wlz
8882_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8882_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38156
Entity Detected:March11, membrane-associated ring finger (C3HC4) 11 ( MGI:3608327)
Sequence:sense strand is shown

>T38156
TGCGAGCTGTGCTGTTATAGATACCACGTCACAGCCATTAAAATGAAGCAACCTTGCCAGGTAGACACAA
TGTTCACTCTTCCAGGAATAATTTTGTTAACACGGGTACACTTGAATTTTGTTTTGGCTTAAATGGTCTC
CTTAATACTACTTAAGAATATTTAATACAAGTTGCAGTAAGACCAAGATGTAGAATTTGTAGGTGGTGCT
TAGAGAAAGCCATGACATTCTTTCTGAGAAACTCCAAGGGCATTTTAACCAAATGATTTCCTTTATAATT
TGTTGTTTACTGATTAAATTATACTGAATTCTGTTTCTTAAAGGCTATATTGGTAAAAATGAATCCACAT
GAGTCTGTTTTATTAATAAGAGATGGTATACAGCTTATTTAGCAGACTTTTTTTTTTTTTTGCATTTTAA
ACCATACCTAGCTCAATTTATGGAAAAGAGATGCAAATGAAGTGGCTGTTTACCAGTGTGGAAACTTCCA
GTTGAGTCTGTCAGCTCAAGACTAAACATTCTTCCTGCTTATGTTTTCTGTTCCATAAGAGTTTTAAAAA
ATTTTTTTCCACATTAACGCTTGGTGCCATTCAGTTACATATGAGACGCAGGCATGTTCCCTGCAGCCGA
TCTCCATAATGCTGGTGAAGTTCTACCTAGTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 105287. Forward Primer - name:105287_F_cDNA_9630025C22, sequence:TGCGAGCTGTGCTGTTATAGAT; Reverse Primer - name:105287_N_SP6_cDNA_9630025C22, sequence:AACTAGGTAGAACTTCACCAGCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8883 same embryo
 EMAGE:8881 same embryo
 EMAGE:8879 same embryo
 EMAGE:8884 same embryo
 EMAGE:8880 same embryo
 EurExpress:euxassay_010112 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS