EMAGE mouse gene expression spatial database

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EMAGE:10299

Pex5 peroxisomal biogenesis factor 5 ( MGI:1098808)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000323. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000323_01	euxassay_000323_02	euxassay_000323_03	euxassay_000323_04

euxassay_000323_05	euxassay_000323_06	euxassay_000323_07	euxassay_000323_08	euxassay_000323_09

euxassay_000323_10	euxassay_000323_11	euxassay_000323_12	euxassay_000323_13	euxassay_000323_14

euxassay_000323_15	euxassay_000323_16	euxassay_000323_17	euxassay_000323_18	euxassay_000323_19

euxassay_000323_20	euxassay_000323_21	euxassay_000323_22	euxassay_000323_23	euxassay_000323_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10299_wholemount_strong.wlz

10299_wholemount_moderate.wlz

10299_wholemount_weak.wlz

10299_wholemount_possible.wlz

10299_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10299_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 09 10 11

cerebral cortex

moderate

regional

moderate expression: see section 01 02 03 04 05 06 09

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 06 07 08 09

cerebellum

moderate

regional

moderate expression: see section 03 04 05

midbrain marginal layer

moderate

regional

moderate expression: see section 08 09

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08

vagus x ganglion

moderate

regional

moderate expression: see section 07 08 09

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08

spinal cord

moderate

homogeneous

moderate expression: see section 11

dorsal root ganglion

strong

homogeneous

strong expression: see section 09 10

inner ear

moderate

regional

moderate expression: see section 02

tail dorsal root ganglion

moderate

homogeneous

moderate expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3544
Entity Detected:	Pex5, peroxisomal biogenesis factor 5 ( MGI:1098808)
Sequence:	sense strand is shown >T3544 GGTAAGTCTCTCCTTGCCCCATCTTCCTGGGAATTGGTAACAGTCCAGCTTCCTTGGGCAGTTGTGTGTA AGAGGCTCCCCTGAGCATCTGTGCGACGCCAGCTGGGATGGATGTTAGGAGCTGCCCTGCTCGCTGAAAT GGTGATGCGGCTCCCTAGAGCCGAACTTGGAACAAGTGCTCGCAGCTCGGTCACTCCGGGAGCGAGCATG GATCTTCTGTGGGTGGACACTGAAGCAGCAGATGCTCATGTGGTTACTGTACTGTCCTCAGGGCCAGGGC TTGTCACTGCAGGTGGCCTGGCCTATGGGGGAGGGAGGTTCATTTGTGGAATGAGCCCAGAGATGCGACA GTAGTGATACGGTCAGCACTGCAGTCCTCTCTTCTTGGGAGACTTGGAAGTAGGAACTAAACAAACTCTT GTAGCAACCTGGTTCAGAAGTTGCATTCTTGCCAAATTCCCAACCAAGGGAGGGTTCAGTGTTACCATAA GCCTTCTTGAAACCTTTATAGGGGATATCATT
Notes:	The probe template was PCR amplified from IMAGE:3166734 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3166734 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10302 same embryo
	EMAGE:10298 same embryo
	EMAGE:10301 same embryo
	EMAGE:10303 same embryo
	EMAGE:10300 same embryo
	EurExpress:euxassay_000323 same experiment
	MGI:4827160 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source