EMAGE mouse gene expression spatial database

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EMAGE:10301

Gapvd1 GTPase activating protein and VPS9 domains 1 ( MGI:1913941)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000318. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000318_01	euxassay_000318_02	euxassay_000318_03	euxassay_000318_04

euxassay_000318_05	euxassay_000318_06	euxassay_000318_07	euxassay_000318_08	euxassay_000318_09

euxassay_000318_10	euxassay_000318_11	euxassay_000318_12	euxassay_000318_13	euxassay_000318_14

euxassay_000318_15	euxassay_000318_16	euxassay_000318_17	euxassay_000318_18	euxassay_000318_19

euxassay_000318_20	euxassay_000318_21	euxassay_000318_22	euxassay_000318_23	euxassay_000318_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10301_wholemount_strong.wlz

10301_wholemount_moderate.wlz

10301_wholemount_weak.wlz

10301_wholemount_possible.wlz

10301_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10301_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 11 weak expression: see section 12 14

nervous system

weak

homogeneous

weak expression: see section 12 13 14 15 17

central nervous system

moderate

homogeneous

moderate expression: see section 19

telencephalon

moderate

homogeneous

moderate expression: see section 19 20 21 22 weak expression: see section 18

facial vii ganglion

moderate

homogeneous

moderate expression: see section 17

glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 07 17

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 03 04 06 07 08 17 18 20 weak expression: see section 16

vagus x ganglion

moderate

homogeneous

moderate expression: see section 07 08 09 17 18

vestibulocochlear viii ganglion

moderate

homogeneous

moderate expression: see section 07 09 11 17

spinal cord

moderate

homogeneous

moderate expression: see section 10

dorsal root ganglion

moderate

homogeneous

moderate expression: see section 08 09 10 11 17 18

inner ear

moderate

regional

moderate expression: see section 22

metanephros

moderate

regional

moderate expression: see section 08 09 19 weak expression: see section 06 07

nucleus pulposus

moderate

homogeneous

moderate expression: see section 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3552
Entity Detected:	Gapvd1, GTPase activating protein and VPS9 domains 1 ( MGI:1913941)
Sequence:	sense strand is shown >T3552 GGCCTCNAGGCCAGATTCGGAACGAGGAAAACCAGAACAGAAATCTTTTAGGAAACGTCTAGATCTTGGT TCATGAATTCCCTGTTCGTCCTTCTCATTTTTAGAAAGAACAGTGTGCCCCTTCCAAGTTGCTGTGTGAG CGTTCGGTCGTGAGGCACCTGCACGGAGGGTCTCAGGAGATGCGCAATACGGGCTCCCTAGATACACTCT CCTAAGGAAGAGCTAAGAAGGAAATGTAAATCTAATATATATTTATATTTGATGTACTATGGGCATCTTC AGACTCTAATAAACAACAAGGACAGTTGCTGAGAGTAGGCTGTGACATTCCCTCTTGTGAAATGGTTTCC TTGACAAAATTTAAGCTGAGCTTAAAAGCAAAAGCAGAAATATTTATTAGTCTGGAGTAGAGTTTATCGC TCTGCATGTGTGGAGTTTGATGGGGAAGTCATGAAGGCGTGTACCACCATGGACATGTGTTGAGCCTGCG CATTGGGGCAGACTCCACCATGTGGACAAGCAGTACGTGGCATGGCAGGTGTGCTCATGCGTCCTTCCTT TGCACCTGCGCACTGAG
Notes:	The probe template was PCR amplified from IMAGE:3166837 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3166837 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10302 same embryo
	EMAGE:10298 same embryo
	EMAGE:10303 same embryo
	EMAGE:10299 same embryo
	EMAGE:10300 same embryo
	EurExpress:euxassay_000318 same experiment
	MGI:4824994 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source