EMAGE mouse gene expression spatial database

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EMAGE:10641

Arhgap24 Rho GTPase activating protein 24 ( MGI:1922647)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003218. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003218_01	euxassay_003218_02	euxassay_003218_03	euxassay_003218_04

euxassay_003218_05	euxassay_003218_06	euxassay_003218_07	euxassay_003218_08	euxassay_003218_09

euxassay_003218_10	euxassay_003218_11	euxassay_003218_12	euxassay_003218_13	euxassay_003218_14

euxassay_003218_15	euxassay_003218_16	euxassay_003218_17	euxassay_003218_18	euxassay_003218_19

euxassay_003218_20	euxassay_003218_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10641_wholemount_strong.wlz

10641_wholemount_moderate.wlz

10641_wholemount_weak.wlz

10641_wholemount_possible.wlz

10641_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10641_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 17 18 19 20 21 moderate expression: see section 07 08

vibrissa

moderate

regional

moderate expression: see section 04 weak expression: see section 05 06 17 18 19

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 05 06 07 08 09 11 12 13 14 15 16 17

4th ventricle lateral recess

moderate

regional

moderate expression: see section 03 04 05 06 07 09 10 11 12 14 15 16 17 18 19 20

medulla oblongata basal plate

moderate

regional

moderate expression: see section 04 05 06 07 16 17

pons

moderate

regional

moderate expression: see section 06 15 16

pons ventricular layer

strong

regional

strong expression: see section 11

facial vii ganglion

weak

regional

weak expression: see section 03 18 19 20

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 17

trigeminal v ganglion

weak

regional

weak expression: see section 02 03 04 05 06 07 15 16 17 18 19 20

vagus x ganglion

weak

regional

weak expression: see section 07 16

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07 16 17

ventral grey horn

moderate

regional

moderate expression: see section 10 11 12 13 14

dorsal root ganglion

weak

regional

weak expression: see section 07 08 09 10 14 15 16

viscerocranium

moderate

regional

Expression in the turbinate bone.

meckel's cartilage

moderate

regional

moderate expression: see section 02 03 04 weak expression: see section 16 17 18 19 20

lower jaw molar

moderate

regional

moderate expression: see section 04 weak expression: see section 05 06 16 17 18 19

upper jaw molar

moderate

regional

moderate expression: see section 04 weak expression: see section 05 06 16 17 18 19

liver lobe

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

kidney calyx

strong

regional

strong expression: see section 04 05 06 07 08 09 14 15 16 17 18 19

axial skeleton

moderate

regional

moderate expression: see section 12 weak expression: see section 11 13

cranium

strong

regional

strong expression: see section 21

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4833
Entity Detected:	Arhgap24, Rho GTPase activating protein 24 ( MGI:1922647)
Sequence:	sense strand is shown >T4833 GAGACGTGGTTTCCATTCCTGGGTGATGGAGTCATGGGAGAAACTGCATTGAGCAAACGAAGTGGGACCT GAGCCGGCTCCAGTGTTCACTAAGCCGCTCAGGGCAGAGCAGCTGCTGTCTCTCCCAGCCTGCATCTGTC ACTGACCACTGAAGTGTGTGTGCAAGGAGGTGAACGAGATCGGATGACAGCCAATCATGAGAGCTATCTC CTCATGGCGAGCACCCAGAATGACATGGAAGACTGGGTGAAGTCCATCCGCCGAGTCATATGGGGACCTT TTGGAGGAGGTATTTTTGGACAGAAACTGGAAGACACAGTCCGATATGAAAAGAGGTACGGGAACCGGCT GGCACCCATGCTGGTGGAGCAGTGTGTGGACTTCATCCGACAGCGGGGGCTGAAGGAAGAAGGCCTCTTC CGGCTGCCGGGCCAGGCGAACCTGGTCAAGGAGCTCCAGGATGCGTTCGACTGTGGAGAGAAGCCCTCCT TCGACAGCAACACCGACGTCCACACTGTGGCATCCCTGCTGAAATTGTATCTCCGAGAACTTCCAGAGCC CGTCGTTCCTTATGCAAAGTATGAAGACTTTTTGTCCTGCGCCACGCTGCTCAGTAAGGAGGAAGAAGCA GGTGTTAAGGAGCTAATGAAACAAGTGAAGAGTTTGCCTGTGGTTAACTACAACCTTCTGAAGTACATTT GCAGGTTCCTGGATGAAGTGCAGTCCTATTCAGGAGTTAACAAGATGAGCGCACAGAACTTGGCAACTGT CTTCGGTCCTAACATCCTGCGCCCAAAGGTGGAAGATCCCTTGACCATCATGGAGGGCACTGTGGTGGTC CAGCAGTTAATGTCAGTGATGATTAGTAAGCATGATCGACTCTTCCCCAAAGACACAGAACCACAAAGCA AGCCCCAAGACGGCCCGAACAGCAATAATAACGATGGTCATAAGAAAGCCACCATGGGTCAGTTGCAGAA CAAAGAAAACAATAATACCAAGGAGAGCCCCGTCAGAAGGTGCTCTTGGGACAAGCCTGAATCCCCCCAG AGAAGCAGCGTGGACAATGGATCCCCCACAGCCCTATCAGGCAGCAAAACCAACAGCCCAAGGAACAGTA TCCACAAGCTGGATATTTCCAGGAGCCCCCCTCTCATGGTCAAAAAGAACCCAGCCTTCAACAAGGGCAG TGGGATTGTCACCAACGGGTCATTCAGCAGCAGCAATGCAGAAGGTGTCGAGAAGCCTCAAACTACTCCT AATGGGAGTTTACAAGCCAGAAGGACCTCTTCACTGAAGAGTTCTGGTACCAAAATGGGCACTCACAGTG TACAGAATGGCACTGTCCGCATGGGTATTCTGAACACTGACACACTAGGGAATTCCTTGAACGGCCGGAG CATGAGTTGGCTGCCCAATGGCTATGTGACCCTGAGAGATAACAAACAGAAGGAGCCAGCAGGAGAGTCA GGCCAGCACAACAGACTCTCCACCTACGACAATGTCCACCAGCAGTTCTCCTCGATGAGCCTCGACGACA AGCACAGCGTGGACAGTGCCACGTGGTCCACCTCCTCCTGCGAAATCTCCCTCCCTGAGAACTCCAATTC CTGCCGCTCCTCTACCACCACGTGCCCAGAGCAAGACTTCTACGTTGGGAACTTTGAGGACCCTGTTTTA GACGGCCCACCTCAGGATGATCTTTCCCATCCCGGGGACTATGAGAACAAAAGTGACCGCAGGAGCGTAG GGGGCCGAAGCAGCCGCGCCACGAGCAGCAGCGATAACAGCGAGACATTCGTGGGCAACACCAGCAGCAA CCACAGTGCACTTCACAGTTTAGTTTCCAGCCTGAAGCAGGAGATGACCAAACAGAAGATCGAGTATGAG TCCAGGATAAAAAGCTTAGAACAGCGGAATTTGACTTTGGAAACGGAAATGCTGAGCCTCCATGACGAAC TGGATCAAGAGAGGAAAAAGTTCACCATGATAGAAATCAAGATGAGAAACGCTGAGCGCGCAAAGGAGGA TGCGGAGAAAAGGAACGACATGCTCCAGAAGGAGATGGAGCAGTTCTTCTCCACATTTGGAGACCTGACA GTGGAACCCAGGAGAAGCGAGAGAGGAAACACAATCTGGATCCAGTGAGCCTTTCTCTGTCCTGGACCGC TCGCTCCGCGGAGGACTCTGGGGACTCCGGGGCAAACAGTGTTGGGGAACAGGTGTGGGATCAGGTGGTT GGTCTGTGTAGAGCTTCATCTGGTGCAGGAATCTCATTTCCAGACATCACTATCCATATCTGCAGTGTGT ACCAAAGTTATATCATGCTCCATAATGCTACTGTCAAGTGTTACAACTGGATATGTGTATAGAGAGTAGT TTCTAAGATGTCAATAAGAGTAAGAAGCATATATCTCAATGATTATTTTATTGCAAGTCCTGTATTTAAA TGTCGAATCAATACTTTGTTGCAATTTAGCTTGCATTCAAGCTTCACCCCGTGCACTTAACATAAGCTAT TTTTGGCATCGTGTTATCATCTGCTTATTTTATAGATCAATATTTTTATTTCCCCTTTTGTCGAGGAAAT GGAGATAAGAAGAGATATAAATATATAAATATATATAAGATGAGTTATTAAAGTCAAAAGAATACTTTGT GGCTGTGCTGTTTGTGCCAATAGACCTTGCCATGACCAAAAAAGAGCAATGTAAAACAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:5064885 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5064885 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10637 same embryo
	EMAGE:10642 same embryo
	EMAGE:10640 same embryo
	EMAGE:10639 same embryo
	EMAGE:10638 same embryo
	EurExpress:euxassay_003218 same experiment
	MGI:4823220 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source