EMAGE mouse gene expression spatial database

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EMAGE:10709

Zfyve27 zinc finger, FYVE domain containing 27 ( MGI:1919602)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003201. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003201_01	euxassay_003201_02	euxassay_003201_03	euxassay_003201_04

euxassay_003201_05	euxassay_003201_06	euxassay_003201_07	euxassay_003201_08	euxassay_003201_09

euxassay_003201_10	euxassay_003201_11	euxassay_003201_12	euxassay_003201_13	euxassay_003201_14

euxassay_003201_15	euxassay_003201_16	euxassay_003201_17	euxassay_003201_18	euxassay_003201_19

euxassay_003201_20	euxassay_003201_21	euxassay_003201_22	euxassay_003201_23	euxassay_003201_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10709_wholemount_strong.wlz

10709_wholemount_moderate.wlz

10709_wholemount_weak.wlz

10709_wholemount_possible.wlz

10709_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10709_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 21 22 23 24 weak expression: see section 06 07 08 09

facial vii ganglion

moderate

regional

moderate expression: see section 05 23 24

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 09 20 21

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 19 20 21 22 23 24

vagus x ganglion

moderate

regional

moderate expression: see section 10 19

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 09 10 19 20 21

cervico-thoracic ganglion

weak

regional

weak expression: see section 11 12 18

cervical ganglion

weak

regional

weak expression: see section 11 18

thoracic ganglion

weak

regional

weak expression: see section 14

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 13 17 18 19 20

not examined

regional

not examined expression: see section 02 03 04

neural retina

moderate

regional

moderate expression: see section 02 03 04 weak expression: see section 01

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 13 14 16 17 18 weak expression: see section 09 10 11 12 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5362
Entity Detected:	Zfyve27, zinc finger, FYVE domain containing 27 ( MGI:1919602)
Sequence:	sense strand is shown >T5362 GGCAGACGGTTCCCGGGTGGACCTGCGACTTCGGAGCGTCGGTGGGAGGTGGCGTGGCGTGGCGTGGGGT GGGGCGGGCCTGGCCTGAGGGACCGCTCCGGGGCTTGGTGGCCCTTCTGTCCCCCTGGCCGTTCCTGTCT CTTCCTTTCCTGGGACAGCGTCCGGAATGCAGACTTCGGATCGGGACCTGAGTGGGCCGGAGGCGAGCCC CAGCGGGATGCCTGAGGTTCTCTCTGAGTGTCCACCTGCCCCTACCAAGTCAGCAGCGTTTGATCTCTTC AACCTGGTCCTGTCCTACAAGAGGCTGGAGATCTACCTGGAACCCCTGAAGGATGCAGGGGACGGTGTTC GATACTTGCTAAGGTGGCAGATGCCTTTGTGTTCCTTGCTGACTTGCCTGGGCCTCAACATCTTGTTCCT CACTTTGAACGAGGGTGCGTGGTACTCCATGGGTGCCTTGATGATCTCGGTGCCTGCCCTACTGGGCTAC CTTCAGGAGGTGTGCCGGGGACAGCTGCCAGAGTCGGAGCTGATGCGGAGGAAGTACCACAGCATAAGGC AGGAAGACCTGCAGAGAGTTCGCCTTTCCCGAGTGCACCTTTCCCGCCCTGAGGCTGTTGCTGAGGTGAA GAGCTTCTTGATCCAACTGGAAGCCTTCTTGGCCCGCCTGTGCTATACCTGCGAGTCAGCCTACCGTGTA CTTCACTGGGAGAACCCCGTGGTGTCCTCACAGTTCTATGGCGCTCTTCTGGGCATGGTGTGCATGCTCT ACCTGCTACCGCTCTGCTGGGTCCTCGCCCTGTTAAATAGCACACTCTTCCTGGGAAATGGGGACTTCTT CCGAGTGGTGTGTGAGTACAGGGCCTGCCTGCAGCGGAGGATGAACCCCCGGCAGGAAGAGTGTGCCTGT GAGAGCTCAGCACTGCAGGGGGCCGGAGGGAGGGGCCTGCTGGACAGCTCGCCTGCCCCCACACCCACCG AGGACCTCACGCCAGGCAGTGTGGAGGAAGCTGAGGAGGCTGAGCCAGATGAGGAGTTCAAAGATGCAAT TGAGGAGACCCACCTGGTGGTGCTGGAGGATGAAGAGGGCACCCCGTGCCCAGCAGAGGATGAGCTGACC CTGCAGGACAATGGATTCCTCAGCAAGAATGAGGTGCTGCGCAGCAAGGTGTCCCGGCTTACAGAGCGGC TTCGCAAGCGTTACCCAACCAATAACTTCGGGAATTGTGCAGGCTGTGCCGCTACGTTCTCCGTGCTGAA GAAGAGGCGGAGCTGCAGCAACTGTGGGAACAGCTTCTGCTCGCGGTGTTGCTCCTTCAAGGTGCCCAGG TCCTCTATGGGAGCCACAGCTCCTGAAGCCCAGAGAGAGACTGTGTGTGTGTGCGCCTCCTGCAATCAGA CCCTGAGCAAATGAGAAGAGAAGCCAGGCTCCAACTGTCCTGGGGCCAGCAGTGACCCTACTGTCCCAAC TCTGGGCCTCTGAGGCCTGTGCTGGGCAAACGTAGCCTGACAGCTAGGTCCTTCCTGCCCTTCCCCTGCT GCCTCCAGCTGGGCTGGTGATGCTCTAAGGCCCAGGGGGAGAGGAGCCCCTGGGGTCCCGGCTCTTGGTT CTGCTCTATCCTATACCCTGTTAACTCAGGGCTAGCGAGGGAGTGTCCACCAGAGGGCAGAATTGAGCAC AGCCTGCTCTCATCTGGGCTCTGGTCGTCAAGGTCAGGCTGGCTAGACCTTAGGATCCAAGAGACTTGAG GTTGGAAAAGAGGTTCTTCCCTGTTGGGTACTTGTCAGTCTCTCAGCCCCAGAGAGCCTGCGAGGGGACT TAGAGCTCCATGGCCTGCCTTTGCCTTTTGAATAGCCTCAGGACTGGCGTTCCCAGGTGTCCCAAGGAGA GGGAAGGTGAGGGTGCTGCTCAAGTCCTTCAGTGTCAAGTGGATGGCAGGATGGGGAAGCTCTGCTTACA GAATCTTAACTCCCATGGTTCTCTGGTGTCCTCAGCCCTGCTGGGGTGGCTACAGGACCCTCACATCACT CTTCCATCCTGCTCTGAGTCTAGGGCACCCCACCTTTCTCCCACTGCCACATATCTCTAGCCCTTATCTC ATGGAACATGCGGCCAGGTGAGTGGAGCAGAGAGACTGCAAGCCTTTGAGGTCCAGGGACGTGTAGGACT GCCCGGATTTCAGGGCTGTTCCTGGAGTAGAGATAGCATTGTAGGATAGGGTCAGTCACATGGCCAGTTT TATGTTCCCTCTGACCTGCCAAGGGTGCTGTGCCTCTTCTTAAGCCATGAGCTGGCTCAGGCCCCTTCCC CTGGAGCTTTTTCAGAGTATTTATTTATTTATTTCATGGTTCCTGAGAGCACGCAGCACAGGGTAGGATG AAGAAGACAAGGGGCTTCTGCCTGAGACCCTTCTGCAGTCATGGACTGCCCTTGGAGCTGATCTTCAGGA ACTGTTTCTGCTGCCCAGTCATGGGTGTGGTGCCCACACCTGAACCATTTGAGGTCCTCCTTGCTGATAG GCCACACACACACAGCAGTAGAGCACTGAGGGTTTGGCAGTGGGGACCAAGCAGGTGAGGTGTACTCCTT TGTCCTGGTGAGAGATGCTCACCTCTGCGTATTTATACTGTAGAGCAGTAGACAGGAACGCTGTGGAGAA TTGTCAATAAAAGGCCCCATGCTTATGGTTTTGTCAGGTCTCCTAGTGCTGTTCAGGTCTTTCATGTTGC CCAGGGTGGGCTGTAGGTGGGCTTTGCACACCTCCTGTGGCAATTCGTGAGCCAGCCAGGCCCAGTGGAA AGCTGTGCCTTATCAACAGGAGGGGTGGGCTTTATCTTGTGACCAGACCCACGGTCTTGGATTGAGGTTG GGACCAGAACTTAACTATAAATAATGCAGGCTTCAGAATGGAAAGGTTTTAGCTAATCCTCTGGGAAAGA CAGAAAGGCCAGCGCTGCAGGAGGCTGAGAGCTGGTAAGCAGGTTTCTGGTGGGGTGAGGTGCAAGTTGG CCCTGTGATCAGGCTAGGCTGGAGCTGGGCACTGCACACATTATGTTAGCGCTGGCACCAAGCTCATGAG GTAGGCTCTGTTTTCCACATGGTAAGGCCGGCAGTGCACAGCAGCAGCACTGTGAGCCAGCTCTGAGCTC AGCCCCACCTACCAAGGACCCATGTGCTATCATCCTGTAGCCTTTAGAAAGAATGCACATCTGTCCTGTG TCCTTATAGCAAAAGCCACTGCTGCCTCCTCTTCCTCCTCTCCCTCCTCTTCTTCCACCACCTCCTTCAA CAAAGAATGATTTTAACTTTTTTTTTTTTTTTTTTTTTTTAAGGTATCAGGCCATCTTATTGAAAGGTGA CTGTGTAGCCGGGCGTGGTAGCGCATGCCTTTAATCCCAGGATTTGGGAGGCAGAGGCAGGCGGATTTCT GAGTTCAAGGTCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGCCAGGGCTACAGAGAAACCCTGTCTTG AAAAACCGAAAAACAAAACAAAGCAAAAGGTGACTGTCACTGCTGAAAAAGGCTGGCTGTAGTCACAGAT GTGAGACAGGGGGCTTGCCTTTTTTGTTTCCTGGAGGTCCCTGTGAGAACAGGTGGGGAGGCTGTCTTCA GATGCAGGTTCCTCTTAAGAGAGCAGAGCTAACAGGGTTCAGCAAGGTTGGTGATGGTGTCACCATGGAG CCTGTGGTACAGAGATCTCAATGTGGAATGATAGACAGCTTGCACGTTGTAGAATTGGATGTCTGTGTCA AAATACACCTTCCAATTTGCTGGTACCCAGGAACAGAGACAGAGAGCCAGGTTGTGTTAGAGTCCTGGCA TGTGGCAGGGCAGGTTTGGAACTCTGGCATCTAGCCTGACTTGGGCTCAGGTCTGTTGAGTCTTTTGTGG TCTAGTATGTGGATGTCTTCAGAAGGTCTTGGCAGACCTGGAATAGGTGATGATTTTGGGGTGTTTTCTG TAGCCCCTGGGGCTGAAGCCAAATGAATCTCCTTTTTCAGAGATCTGTGTCTCTTCTCACAGCTGGCCCT GTTCACAGGTCATCCTGCCAGGGACTATACCTAGCCAGTAGCTCATGTCTTACAGTGTCACAGTTTGTCT AGTTCTTGGATATTCTAAATAGGTAAATAGAGGTACTGGGGTGGGGGGTGTTAAGTTCCCCACCCAAAGT GGAGTGGGAAACACTTGTGTTACAAGTGTTCATAGCAGCTATGGTCTAAGAAGCCTTGGGTAAAGGCCAT GTTCTGGTCCCCACTCTCTAAGGAGCTAGCCACTGAGCTAACAGACTGGTCTGCCCCACCCCCACCCTCT TAGCACCATCCCCCTTCAGTCTCTACCAGAACAGAAGTGGCCTTGTGGCTGGAGCCCCCACCTTCCCTAG CTCCCAGTGACCCCACCTCCTGCCAGCAACACCCCAGCAGCACATTCCATTCTCTGGCACTGCTGGTGGC TGGGGATTCCGGGCTCCTTTGGCGGTGAGCCTTCTTTGGGGCTCCAGCAGAGGCTGTTTGGGCTGGGACA CTAGGCTCCAGCCACACACATTCCAGCAACAAACACCCCCCACATTAGATTTCCCAAGGAGTGAACTTTA GCCAGGATTACAGATGGCTGCACAGTTGTGGGATGTGAGCTTGCTTTGTGCAGACCTGAGGCTGGATGCT AGTAGGGGTGAAAAGGAATGGGTGGGAGCGGCAGGCTTCTCTGCTGCTGCCTGGGCTCTTGTAGCATCAC GGAAGCAGCTGAAGGCAGCCTTTGAGATTGCCTGTCCCGTCCTATCTCACCTCTGTAGAAGGTGGTAATA GTCCTCAGACTTTTAAGCTGTAGGCTGTGATTCCGTGTGGTATCCCGTAACTGCATGTGAGGATTGGGAA AAGTTTGGTGACAGTAAAAGGCTATCAGACACCGAGCAACTAAAAAACAATTCAAAACCAAACTTGAAAA TCAGTTGGAGGTGTTTGTGGAGTCCTGTCCTGAGCATCGCAGTCTTGGTTCTGAACACACAACAGGCACC CTGTGCCCTCTGCCTCCCCTCCTGCCACACAGCCAGTGAACACTCCTTGAATCACACAGATGTGAGACCA CTGCATATTGTGAACTGCAGTGTTTTTACTGTCCAGTCAGCTCCTGCTTTTACAGGAACACAATGGCTTA CAGAAAATAAGGACATACTATTGCCCTGCTTGCATGTAAATATCAAAAAAGTACATCTTTGCATCACATT ATATTGGAATACTTGATTTTAAAAATTAATGTTTGTGGACTAGTGAGATGACTCATTAGGTAAGTCCTGG TCACATGGGCTGGACCCCTGAATTTGATCCCTGGAACCTGTGTTAAAAAGCCTGATGTGTTGCGTCTGTA GTCTCAGTGTTCCTATAGCAAGATGGGAAGAGACAAGAGGATTGCTCTGGAGTGTGCAGGAGGCTGGCAT AAACCGGAGACTGCCTCAAGAAGATTGAAGGACAGAAGCAGTTCCTCTGGCATCTACACTGTAGCACAAG TAATAACAAAAATTAAAAAAGAAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAGGGCG
Notes:	The probe template was PCR amplified from IMAGE:5363403 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5363403 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10710 same embryo
	EMAGE:10712 same embryo
	EMAGE:10714 same embryo
	EMAGE:10711 same embryo
	EMAGE:10713 same embryo
	EurExpress:euxassay_003201 same experiment
	MGI:4829350 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source