EMAGE mouse gene expression spatial database

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EMAGE:10714

BC031353 cDNA sequence BC031353 ( MGI:2387648)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003223. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003223_01	euxassay_003223_02	euxassay_003223_03	euxassay_003223_04

euxassay_003223_05	euxassay_003223_06	euxassay_003223_07	euxassay_003223_08	euxassay_003223_09

euxassay_003223_10	euxassay_003223_11	euxassay_003223_12	euxassay_003223_13	euxassay_003223_14

euxassay_003223_15	euxassay_003223_16	euxassay_003223_17	euxassay_003223_18	euxassay_003223_19

euxassay_003223_20	euxassay_003223_21	euxassay_003223_22	euxassay_003223_23	euxassay_003223_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10714_wholemount_strong.wlz

10714_wholemount_moderate.wlz

10714_wholemount_weak.wlz

10714_wholemount_possible.wlz

10714_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10714_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 04 05 06 07 22 23 24 moderate expression: see section 08 09 10 21

hindlimb digit 1 mesenchyme

moderate

regional

moderate expression: see section 05 24

hindlimb digit 2 mesenchyme

moderate

regional

moderate expression: see section 04 05 06 23 24

hindlimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 04 05 06 23 24

hindlimb digit 4 mesenchyme

moderate

regional

moderate expression: see section 04 05 06 07 23 24

hindlimb digit 5 mesenchyme

moderate

regional

moderate expression: see section 06 07 23

lower leg mesenchyme

moderate

regional

moderate expression: see section 01 02 03

upper leg mesenchyme

moderate

regional

moderate expression: see section 02 03

adrenal gland

moderate

regional

moderate expression: see section 09 10 11 18 19 20

pituitary gland

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

meckel's cartilage

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 19 20 21 22 23 24

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 13 17

lower jaw molar

moderate

regional

moderate expression: see section 06 07 08 09 10 20 21 22

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 13 17

upper jaw molar

moderate

regional

moderate expression: see section 06 07 08 09 10 19 20 21 22

testis

moderate

homogeneous

moderate expression: see section 06 07 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5365
Entity Detected:	BC031353, cDNA sequence BC031353 ( MGI:2387648)
Sequence:	sense strand is shown >T5365 CTGCCTTTGTGAGAAGCCTGCACGGGGAAGCGCAGCACCCAGCGCTTGCTCCACTCGCTCGCTCTCTCCT CTCCCGTGCTTTCCCTCCGCGGCTGACAGGGTAGCAGCTGACAGCCGGAGGCTTCCAGTATGGTTTTCAG TGGGAACAAAGGGCTCCGCAGACAAGATGCCTTGGATGAGTATTTTGAATATGACGCAGAGGAGTTCTTG GTCTCTTTGGCCTTGCTGATAACTGAAGGACGAACCCCGGAGTGCTCTGTGAAAGGCCGTGCAGAGAGTT TCCACTGCCCTCCAGCGCAGTCTCGCTTCCCAGGAACGGCCAGACATGAATGCAGCGACAAGCTGGCCCA GTGTCGCCAAGCCAGACGAACCAGATCTGAGGTCACACTGTTGTGGAAAAATAATCTCCCAATCATGGTG GAGGTGATGCTCCTGCCGGATTGCTGCTTCAGTGACGAGGGGCCTAGTACAGAGGGGGCAGATCTGAATG ATCCCGCCATTAAGCAAGACGCACTGTTACTGGAGAGGTGGATCTTGGAGCCAGTTCCCCGACAGAATGG TGACCGCTTCATCGAAGAGAAGGCACTTCTGCTGGCTGTCAGATCATTTGTGTTTTTTTCTCAGTTAAGT GCTTGGCTGAGTGTTTCTCATGGTGCTATTCCACGAAACATTCTTTACAGAATCAGTGCTGCGGATGTCG ACCTGCAGTGGAGTTTTTCACAGACTCCAACTGAGCATGTGTTTCCCGTCCCCAATGTCTCTCACAATGT GGCCTTGAAAGTCAGCGTTCAGTCCTTACCCAGACAAGCTCATTATCCGGTTTTGACCTGTAGTATTCAT ACTAACATTGGTCTGTATGAGAAGAGAATTCAAGAACAGGAGCTTAGAGCCTGTCAGCACCATGGCCCTG GTGAGGTGGAGTACCGTTGTCCCAGCAGCTCACAGAGCCTATGCAGCAAGCACACATGGACCATGGCACC CGTGAGTGCCCTGCACGTCAGAAGTGGCATGCCTCCCGAGTACACTGCAGCCATTAGAAATGTCAGGCTC TGCCCAGGCACAGGCAGCAAGTCTGACCAGGGGGCACCTCAAGCCAGTGTCCTGGGCTTCAGTGGCACTG GGGGAGAAGTGAGGTCACAGGAGGCGTCAGTGAGAACGTTCAAATCACTGCCTGTGGTCGATTCCAGTGT CTCCAGCTGTCAGAGCTCTCGGCAGCCAGTAGGGGAACCTAACCCTTTAATGGACTCCTTGATTCAGGAT CGACAAGAAGTCATTGCCAGGATTGCTCAGCACTTGATTCACTGTGACCCCAGCTCCTCTAGAATGTCTG AACTCCCCTTTAACACCCAGGAGTCCACGTCACTTAGCCCAAAGCTTCACCCAGTTTCACAGGAAAGTGG GTGTGTGAGGAGATATAAAGAAGCATTCTCTGTCTCCTTTGGGAGTCCAGAGTTTGGCTCCCCAGGAGAC AGCAGGGAGGGGAAAGTCAGAGAGAAGTCAGAAACCAGGCCAGGAGAAACTTGCACTTCTCACAGTCTGT ACCCTCGCCAGCCTGCTGGGGAGGCGAACCCACTGATTGGCTCTTTACTCCAGGAGCGGCAGGACGTCAT AGCCAGGATTGCACAGCACCTAGAACACATCGACCCCGCAGCTCACATTCCCCGGCCTGCATTCAGCAAG CACGACTCCAATTCCATTCCTTCTAAAGTGTTCAGGAGTTCATTTGATGACAAAACCTTGTTGAAGAAGG GCAGAGAGAATGTCTCTGTCTCTGTGTCTCATACAGAAGTGTCCTTATTGGGAGACAGAGGTGATGGGGA AAGCCTCAAACCTAGTAAATGCTTGCGATCTTTCAAATACAGCCCTCAAGAAAAGCCTTTGAAACCTGAA GTAAGAACTCAGCATCAGAACCATCCTGATAGCATCACCCCCACAGCCAGGCAGGAGCTGCTGAACAAGG CTGCTGGCTTACTAACTTCCTCTAACACAGCTCTCTGCAAAGAAAGCAGCTTGGGTTTGGTTAGCAGATT GGAAAGTACATCTTGTAAATTACAACTTAAGGAGCAAGAAATGAGCCATGAAACTGAGAAACAGTGTTTG CATTGCAACAGTATTGACAAACAGATTTGCACAAATACATGTACGGAGAAAATCAACGACGAGCATAGTC CAGGATCTCTCCGTCATCTCCAGTGTGATGATTCAAAAGGTATTGACTCAAAACTAAAAGTGACTCTGCT GGAAATGCCTGACAGTTTGAACAAGAACAAAACTAATTGTTCAAGTAAAGACTCCAAAAGATCCAAGACG TGTGAGCAAAGCATTCGGGTTGGCACAGAAAATGACCTCAGTGAAGAGAATGAGGGTCCCGGAAGCACTG TATCAGACCGACTCAAAACTGAGCAGGAGGCAAAAAGGGACTCAGGTGCTGGAAAACCTCACAGCGTAAA GCACTGTTTGTCTGCAGGCGAGAGGCTGCACAGCGCAGACATGTTGAGGACTACACTGAAACATTCAAGT GTCTGGAGGAAACATAATTTTCATTCCTTGGATGGGACATCAACCAGAGCCTTTCATCCTCAGACGGGGC TGCCTCTCCTCTCAAGTCCAGTTCCTCAAAGAAAAACACAGTCAGGCTGCTTTGATCTAGACGCGTCGCT GCTGCATCTGAGAAGCCTATCCTCCAAAAGCTCTCGGCCATGCCTGAATATTGACGAGGACCCAGATGTT CATGAAAAACCTTTTTTGAGTTCAAGTGCTCCACCAATCACAAGTCTTAGTCTCCTAGGAAATTTTGAGG AATCGGTTCTGAACTACCGTTTAGACCCCCTCGGCATAGTCGATGGCTTTACGGCCGAGGTGGGAGCCAG TGGGACTTTCTGCCCAACGCACTTGACTCTGCCAGTGGAAGTGTCGTTCTACAGTGTTTCCGATGATAAT GCTCCCTCGCCTTACATGGGTGTGATTACTTTAGAGTCCCTTGGTAAAAGGGGTTATCGAGTACCTCCTT CAGGAACGATACAAGTGACCTTATTCAATCCCAATAAGACTGTGGTGAAGATGTTTGTAGTCGTGTATGA CCTACGAGGTATGCCAGCCAATCACCAGACATTCCTGAGACAGAGGACGTTCTCAGTTCCTGTTAAACAA GAGATGAAGAGAAGTATTAACAAAGAGAACGTCCAGCACACGGCACAGTTACTACGCTACCTCATACACC TGAGGTTCCAGAGTTCTAAATCTGGAAAGATCTACCTCCATAGAGATGTCCGGCTCCTGTTCTCTAGAAA GTCCATGGAGGTCGATAGCGGTGCTGCGTATGAACTCAAATCCTACACTGAGTCGCCAACAAACCCTCAG TTTTCACCAAGATGTTGATGAGGGGGACAACTTAAAAGTATTTGCTCAGTACCCAAGCCTGAACATGAGC ATTCACAGAATGGAATACATCGAACGAGAGCCAGCGGAGCAGAGTGGCCCTCCTCTGTTGTCCTGGACCA GATTTTATTAAAGGACCCCTGAAGTGAGACCCGTGATTCCTAAGTGGACTGGAAGCACCCGTGTCGTCGC CCTTTTTGTACTGTTGAAACCACTTCATTGGACAGTTGCAATAGCAAAATCCCCAGTTAGATTAGTGTTT ACACATTTTATTTCTGTTATTTAATATTTAATGTTTTCCTTTATAGTCAAGTGATGTTTGTCTCTAGTGT TCTAATGTAGCACAATCCTATGTAAAATCATACTATGTATTTTTGACATTATTGTTGAAATTGAAATATA TGCATAAGTCTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5363230 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5363230 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10710 same embryo
	EMAGE:10709 same embryo
	EMAGE:10712 same embryo
	EMAGE:10711 same embryo
	EMAGE:10713 same embryo
	EurExpress:euxassay_003223 same experiment
	MGI:4823423 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source