EMAGE mouse gene expression spatial database

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EMAGE:10748

Pamr1 peptidase domain containing associated with muscle regeneration 1 ( MGI:2445082)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001025. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001025_01	euxassay_001025_02	euxassay_001025_03	euxassay_001025_04

euxassay_001025_05	euxassay_001025_06	euxassay_001025_07	euxassay_001025_08	euxassay_001025_09

euxassay_001025_10	euxassay_001025_11	euxassay_001025_12	euxassay_001025_13	euxassay_001025_14

euxassay_001025_15	euxassay_001025_16	euxassay_001025_17	euxassay_001025_18	euxassay_001025_19

euxassay_001025_20	euxassay_001025_21	euxassay_001025_22	euxassay_001025_23	euxassay_001025_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10748_wholemount_strong.wlz

10748_wholemount_moderate.wlz

10748_wholemount_weak.wlz

10748_wholemount_possible.wlz

10748_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10748_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

strong

regional

strong expression: see section 01 02 03 04 05 06 07 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 24

foregut-midgut junction

moderate

regional

moderate expression: see section 14 15 16 17 18

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 13

pons ventricular layer

strong

regional

strong expression: see section 13

midbrain ventricular layer

moderate

regional

moderate expression: see section 13 14 15 weak expression: see section 12

tongue

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

stomach

moderate

regional

moderate expression: see section 09 10

hindgut

moderate

regional

moderate expression: see section 09 14 15 16 17 18

midgut

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20

axial skeleton

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19 20

pectoral girdle and thoracic body wall skeleton

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19 20 21 22

tail

strong

regional

strong expression: see section 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T202
Entity Detected:	Pamr1, peptidase domain containing associated with muscle regeneration 1 ( MGI:2445082)
Sequence:	sense strand is shown >T202 CTGAGCCTAGAGTTTGACTACATGTGCCAATATGACTACGTGGAGGTCCGCGATGGGGATAATAGTGACA GCCCTATCATCAAGCGTTTCTGTGGCAACGAGAGGCCAGCTCCCATCAGGAGCACTGGCTCTTCACTCCA TGTCCTTTTCCATTCTGATGGCTCCAAGAACTTCGATGGCTTCCACGCTGTCTTTGAGGAGATCACAGCG TGCTCCTCATCCCCTTGTTTCCATGATGGCACATGCCTCCTTGACACCACTGGGTCTTTCAAGTGTGCCT GCCTGGCTGGCTACACTGGGCAGCGCTGTGAAAATCTACTTGAAGAAAGAAACTGCTCAGACCTTGGGGG GCCAGTCAATGGGTACAAGAAAATCACAGAAGGTCCTGGACTTCTCAATGAGCGCCATGTAAAAATTGGC ACGGTTGTGTCTTTCTTTTGTAACGGCTCATACGTTCTGAGTGGCAATGAGAAACGAACTTG
Notes:	The probe template was PCR amplified from IMAGE:2648551 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2648551 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10747 same embryo
	EMAGE:10745 same embryo
	EMAGE:10750 same embryo
	EMAGE:10749 same embryo
	EMAGE:10746 same embryo
	EurExpress:euxassay_001025 same experiment
	MGI:4827036 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source