EMAGE mouse gene expression spatial database

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EMAGE:10853

Fam115c family with sequence similarity 115, member C ( MGI:2385258)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003455. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003455_01	euxassay_003455_02	euxassay_003455_03	euxassay_003455_04

euxassay_003455_05	euxassay_003455_06	euxassay_003455_07	euxassay_003455_08	euxassay_003455_09

euxassay_003455_10	euxassay_003455_11	euxassay_003455_12	euxassay_003455_13	euxassay_003455_14

euxassay_003455_15	euxassay_003455_16	euxassay_003455_17	euxassay_003455_18	euxassay_003455_19

euxassay_003455_20	euxassay_003455_21	euxassay_003455_22	euxassay_003455_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10853_wholemount_strong.wlz

10853_wholemount_moderate.wlz

10853_wholemount_weak.wlz

10853_wholemount_possible.wlz

10853_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10853_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb

weak

regional

weak expression: see section 01 22 23

hindlimb

moderate

regional

moderate expression: see section 04 05 weak expression: see section 21 23

gland

moderate

regional

moderate expression: see section 20

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15 16

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 10 17 18 19 20

vibrissa

moderate

regional

moderate expression: see section 03 04 05 22 weak expression: see section 21

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 17

spinal cord lateral wall

moderate

regional

moderate expression: see section 12 13 14 weak expression: see section 15 16

dorsal root ganglion

weak

regional

weak expression: see section 08 09 11 12 13 17 18 19

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 04 23

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 12 13 14 weak expression: see section 15 16

tongue

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07

midgut

moderate

regional

moderate expression: see section 04 05 06 12 13 14 15 16 17 18 weak expression: see section 07 08 09 10 11

lower jaw incisor

moderate

regional

moderate expression: see section 15 16 17

lower jaw molar

moderate

regional

moderate expression: see section 20

oral epithelium

moderate

regional

moderate expression: see section 05 06 07 08 09 18 19 20

upper jaw molar

moderate

regional

moderate expression: see section 20

bladder

moderate

regional

moderate expression: see section 12 13 14 weak expression: see section 15

metanephros

moderate

single cell

moderate expression: see section 08 09 weak expression: see section 10 11 17 18 19 20

lung

moderate

regional

moderate expression: see section 04 05 06 08 09 10 11 12 13 14 15 16 17 20 23 weak expression: see section 07 18 19 21 22

not examined

regional

not examined expression: see section 08 09 11 12 13 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6227
Entity Detected:	Fam115c, family with sequence similarity 115, member C ( MGI:2385258)
Sequence:	sense strand is shown >T6227 CAGCGACAGTCAGCCGGCAGCAACCTCTGTGGTCTGAATTTTCTGTCCTGGTTTGTTCCGGTGTGGGGCT GAGTGTCCGAGCACTAAGACGACGCTGGGAGGAAATCCTTAAAGTGCCAGAAGGCTACTGGACGCCGTTC CCAACAGTGTATCTGCTCACAGCAACGGGCAGAACCATGGCAACAACTCCCGATGCTGCTTTTGAGGCCC TCATGAATGGAGTGACCAGCTGGGATCTCCCTAAAGAATTCACTCCCAGTGAACTCCTTCTTATTGGGGA GGCAGCCTTCCCAGTGATGGTGAATGACAAGGGCCAGGTGCTCATTGCTGTTTCTTTCTATGGCCAAGGC CGCCTGGTGGTCGTATCCCATGAGAGCTACCTGATGCATGCTGGCTTGGCTCCTTTTCTTCTCAATGCAG TGAGCTGGCTCTGCCCCTCCCCAGGGACTCCCATTGAAGTGCATTCTTCCTTAGCATCACTAGTTAACAT CCTACGTGGCTCTGGGATAAATGCCTTGGTTCAGCCTGAGCCTGGAGAGGCTCTGGGAGTTTATTGCATT GATGCCTACAACGACACCTTGACCAAGAAGCTGGTCCAGTTTGTGAAGCGTGGTGGAGGCTTGCTCATTG GAGGTCAGGCCTGGAATTGGGCCAGTCAGCATGGCAGTGACAAGGTGCTATTCAGTTTCCCTGGGAACAA GGTGACAAGTGTGGCTGGAGTCTACTTCACAGATGTCTATGGAGATATAAACCGGTTCAAAGTCTCTAAG AAGATACCCAAGATCCCACTGTATATCAGGTGTTGGGAGGAGCTCAGACATGACCAGGACCAGCTTCTGG ATGGAATCTCTATGTTGGATGTCAGGACTGGGGGTGTCCCCTCACAGCTGTTGGTGCATGGGTCCCTGGC CTTCCCTCTGGGGCTCGATAACTCCTTCCTTAGCTGCTTCCTAGCAGCTGCTCACTATGGCCGTGGTCGT GTGGTGTTGGCTGCACATGAGGCCATGCTCTGCGCTCCTAAGATGGAACCCTTTTTGCTCAATGCTATAC GCTGGTTGTCCAGAGGTCAGGAAGATAATATTGGGGTGAACACACGTCTCAAGAATCTGAATTCCCTATT GTTGAAGCATGGCTTGAAATGCAGTCTGGAGTCCCATTTGACCGATGATATGTGTGTCTACTGCTGTGCA GCTTATAGTGACCAGGAGGCCAAGAAAATACAGGAGTTTGTAGCAGAGGGTGGAGGGTTGCTCATTGGTG GTCAGTCCTGGTGGTGGGCTTCCCAGAACCCAGGCAGCTCTGCTTTAGGTAGTTTCCCTGGGAATGTCAT CCTCAATACTTTTGGCCTCAGCATTTTGCCTCGGACAGTCAGCCCAGGCTGTTTTCCTATCCTTCATATA GATATAAGAAACTATCACTTTCGAGGGGCTCTTTCTGAGTTCCAAGCTATGTTGAACCACAAGGAGGGGA ACTTGGAAAAGAGGTATTCGGGAAAGTTGGGAGTAGATGGAGCAGGCTTTCTCCAGATTCCTGCACAGGG AGTTCCTGCTTACTTATCTGTGCACCGGATCCTGAGGAAGATACTGCGACAGGCAGGCCTCCCAGCTGTG AGCAAGAGTAATCCAGTGTCCAGTCACTCCTATGAGGCTGCGATACTCCAGCTAGCCACAGAGCTGGCAC ACTCTGGGAGTGATTGCTCCCAGATAGCACATAACCTTAGCAGTCAGACCTGCTCCTCCAATCTCTCCTC CTCAGAACACCCCATTACCGTGGAGATCAATGGAACCAATCCAGGTGACCGTGATGTCTGGATGAGCACT GGGCTCTACCTCCTGGAAGGACAAAGCACCGAGATCTCTGTCTCTGAACCTGCTGCCTCTGCAGGTCTGA AGGTGCAGATTGGATGTCACACTGATGACCTTACCTTTGCCATAAAGCTGTTTCGAGCCCCTGTGGTAAC TTACCAGTGCTGTATGAACAGAACCCAACGGTCAGTCTCCTGCCTCTGGGGTGGTCTCCTGTACATCATT GTGCCCAAAGGCTGTCAACTGGGCCCTGTGTCTGTTACCATCACGAATGCAGTGCCTGCCCCATACTACA AGCTAGGTAAGACATCACTGGAGGAGTGGAAGAGCTGCATCCAGAAGAACCTAGGCCCCTGGGGAGAGCT GGCCACAGACAATGTCATCCTGACAGTGCCAACTGCAAGCCTCAAGACTCTGGAGAATCCAGAGCCTCTG CTCCAACTCTGGGATGAGATGATGCAGGCTGTGGCCAGGCTGGCAAGCCAGCCCTTCCCTTTCCAAAGAC CTGAGAGAATTGTCGCTGATGTGCAGCTCTCAGCCGGCTGGATGCATTCAGGATACCCCATCATGTGCCA CATGGAGTCTGTGCAAGAGCTAGTCAGTTTGGCAAACATAAGAAGTAAGGGCCTGTGGGGACCCATCCAT GAGCTGGGCCACAATCAGCAGTGCAGGGGTTGGGAGTTCCCCCCACATACAACTGAGGCCACCTGCAATC TCTGGTCAGTCTATGTTCATGAGACCGTCCTGGGGATTCCCAGGGCTCAGGCCCACCCTCAACTGAAACC TGAAGAAAGAGAGAAGAGAATTAAAGAACACCTTCAGAAGGGAGCACCCCTGCAAAACTGGAATGTCTGG ACAGCACTGGAGACATATCTGCAGCTCCAAGAGGTCTTTGGGTGGGAACCGTTCATCACCCTCTTTGCTG AATACCAAACCATTTTTTACATCCCTGAAGACAATGAGTGCAAGATGAACATATGGCTGAAGTTGTTCTC TGAAAAAGTGCAGAAGAACCTGGTTCCTTTCTTTGAAGCCTGGGGCTGGCCTATCCAGAAGGATGTGGCT GAAGACCTGGCCTGCTATCCTTCCTGGGAGGATCACCCCCTGAGGATGTACATGGGGAGCGAGTAGTAGG GGCCATGGAGCCGGCTGGGGTGGGGAAAGGGCATTGGGGTGGGGTGAAATGGGTGAGATAAGGCATAGTA CTAGCTTCACGGCCTTAACTATTTTTCTCACTGTTTGACTTTAAAATATATTTTGAATGTCCCTTTCCAC ATCCCATCAGCACATCCCAATTTCAGGGACCATTTCCTCAGATACCACCAGTATCCCCTGGCAGATGCTT TGTACATGAAAAACATTAAGAAGATACTTGCTACATGAAAGGTCACTAAAGTCTCCTGACACAGAAGGAA CAGTCAGTGCATGTCCTAAGACCTGCATACAGCGTTTTCTCTGAGTTCCATGGATAATCTTCCACCAGCT AATATCTTCTTATCATAGAGGAGAAAATGGCATGGATGTTAAACAACTTCTTTAGTCAAGACTGAGAAGA GAAAGTGTCAGATTTCACAGATGTCCATAACAGCGATAGATTGTAGATCTTATCCTCAACAGATTGATAA TGTTAACCAAAATTTGCCCAAGAGATAGATGAAGGTTTGTGCTCTTTATTTGCTTTCCTATGTTCATTAC TGAATTTTGACTAAATATCAAATTGAATCTCTAAATCTTTTTCGCAATTAATCTTCCCTTTACCTCAAGA TTCATCTGCCTTGAGAGGAAACCAGCCTTGGAAAAATATTTAAACCAAGGAAGTTCATTTCCCAAACTGG TGGCATTCTTAATTATAATTGGAGGGGGTGACTAGGGAGCTAATTAAAGCTGGAATTAATGGGTTATCTA AGAGACCAAACCAAGTCCTAACCTTGTACACACCACTTCTCTGTAGGATGTAGAGCCCTGTGATGGTGTC TTCTCATTCCTGAGGAGAGACCCTGGCTCCGCCTCTTGTTTTTATGCTTTTGGTAAATTTTTCTGTGGCT GTAATACCCACAGATTATGCTTGTATACGTAAAAACATTTTTTTCTGCTTACAATTTTATAGTTCTGTAT TTACAAATCTTATTTAGGAGAAAATATTGAAGAATAGTGATCTCAGACAAAGTCTGAAAAAGCATGAGTG GGGGAGGAGTTATGAAGTCCCATCCCTAGTTAGAGATCTGTTAGCAGTCTATGGCTGCTGGGAGGAGAGT CGGCTTTCTTCAGCTTTGTGGTCCCTAAGAGGCTACCTATGCCGTACACCAGCACACATATAGGCAGCAC TATGGGCTTTGAAATGAACACATGAATTTAGGAGGGGATAGATATGTGGGGATAGGGGATGAATGAGGAC ATGGGAAATGGTTTTGATCAAAGCACCTTATATACATGTATAGAATTCTCAACTAATAAAAATGAAGAAA ATGCTAGATATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3499593 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3499593 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10858 same embryo
	EMAGE:10856 same embryo
	EMAGE:10855 same embryo
	EMAGE:10857 same embryo
	EMAGE:10854 same embryo
	EurExpress:euxassay_003455 same experiment
	MGI:4824695 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source