EMAGE mouse gene expression spatial database

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EMAGE:10860

Copb2 coatomer protein complex, subunit beta 2 (beta prime) ( MGI:1354962)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003332. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003332_01	euxassay_003332_02	euxassay_003332_03	euxassay_003332_04

euxassay_003332_05	euxassay_003332_06	euxassay_003332_07	euxassay_003332_08	euxassay_003332_09

euxassay_003332_10	euxassay_003332_11	euxassay_003332_12	euxassay_003332_13	euxassay_003332_14

euxassay_003332_15	euxassay_003332_16	euxassay_003332_17	euxassay_003332_18	euxassay_003332_19

euxassay_003332_20	euxassay_003332_21	euxassay_003332_22	euxassay_003332_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10860_wholemount_strong.wlz

10860_wholemount_moderate.wlz

10860_wholemount_weak.wlz

10860_wholemount_possible.wlz

10860_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10860_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 11 12 13 14

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 17 18 weak expression: see section 19

vibrissa

weak

regional

weak expression: see section 07 08 09 22 23

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 11 16 17

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 moderate expression: see section 22

metencephalon rest of alar plate marginal layer

moderate

regional

moderate expression: see section 01 02 03 weak expression: see section 04 05 06 07 14 15 16 17 18

metencephalon rest of alar plate ventricular layer

moderate

regional

moderate expression: see section 01 02 03 weak expression: see section 04 05 06 07 14 15 16 17 18

midbrain ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 weak expression: see section 05

cervico-thoracic ganglion

weak

regional

weak expression: see section 09 10 15

cervical ganglion

weak

regional

weak expression: see section 09 15

thoracic ganglion

weak

regional

weak expression: see section 13 14

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 14 15 16 17 18 19 20 moderate expression: see section 13

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14

liver right lobe

moderate

regional

moderate expression: see section 15 16 17 18 19 20 21 22 23

maturing glomerular tuft

moderate

regional

moderate expression: see section 08 09 10 11 18 19 20 21

testis

strong

regional

strong expression: see section 06 07 08 09 21 22 moderate expression: see section 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6230
Entity Detected:	Copb2, coatomer protein complex, subunit beta 2 (beta prime) ( MGI:1354962)
Sequence:	sense strand is shown >T6230 GTCCACGGGTCGGTGGGTTCCTGAGTGTGTCGCCTTGAGCCTGTGACGGGCAGGGGCTCGCTCGGACAGT CGGAGCCATGCCTCTTCGACTTGACATCAAGAGAAAGCTGACTGCTAGGTCTGACCGAGTTAAGAGCGTG GATTTGCATCCCACAGAGCCATGGATGTTGGCTAGTCTTTACAATGGCAGTGTGTGTGTTTGGAATCATG AAACTCAGACACTGGTGAAGACGTTTGAAGTATGTGATCTTCCTGTTCGAGCTGCAAAGTTTGTCGCAAG GAAGAACTGGGTGGTGACAGGAGCAGACGATATGCAGATCAGAGTGTTCAATTATAACACCCTGGAGAGG GTGCATATGTTTGAAGCACACTCGGATTACATTCGTTGTATTGCTGTTCATCCCACCCAGCCGTTCATTC TCACCAGCAGTGATGACATGCTTATTAAGCTCTGGGACTGGGATAAAAAATGGTCTTGTTCACAAGTGTT TGAAGGACACACGCACTACGTTATGCAGATTGTGATAAACCCCAAAGATAACAATCAGTTTGCCAGTGCA TCTCTCGACAGGACCATCAAGGTGTGGCAGCTGGGCTCCTCATCACCAAACTTCACTCTGGAAGGACATG AAAAAGGCGTGAATTGCATTGACTACTACAGTGGTGGTGACAAGCCATACCTCATTTCAGGTGCAGATGA CCGGCTTGTTAAAATATGGGATTATCAGAACAAAACGTGTGTGCAGACCCTGGAGGGGCATGCCCAGAAT GTGTCGTGTGCGAGCTTCCATCCTGAGCTGCCCATCATCATCACAGGGTCAGAAGACGGAACTGTGCGAA TCTGGCATTCTAGCACCTACCGGCTTGAGAGCACGCTGAATTACGGAATGGAGAGGGTGTGGTGTGTGGC CAGTCTGAGAGGGTCCAACAATGTCGCTCTGGGCTATGATGAAGGGAGCATCATTGTTAAGCTTGGTCGG GAGGAACCTGCCATGTCCATGGATGCCAATGGAAAGATAATCTGGGCCAAGCATTCAGAAGTCCAGCAGG CCAACCTGAAAGCAATGGGTGACACTGAGATTAAAGATGGAGAAAGATTGCCACTGGCAGTAAAGGACAT GGGCAGTTGTGAAATATATCCACAGACCATTCAGCACAATCCTAATGGGAGGTTTGTTGTGGTATGTGGT GATGGGGAGTATATCATCTACACAGCAATGGCATTGAGGAACAAGAGCTTCGGGTCTGCCCAGGAGTTTG CATGGGCCCACGATTCTTCAGAGTATGCAATAAGAGAAAGCAACAGTATTGTAAAGATATTTAAAAACTT TAAGGAAAAAAAATCATTTAAACCCGATTTTGGAGCTGAAAGTATCTATGGGGGCTTCTTGCTGGGAGTC AGATCTGTAAATGGCTTAGCCTTCTATGACTGGGAGAATACTGAACTCATCCGCAGAATTGAAATTCAGC CCAAGCACATCTTCTGGTCTGACTCTGGAGAGCTGGTCTGTATCGCCACCGAGGAGTCATTTTTTATTCT TAAATATCTGTCAGAAAAAGTCTTGGCTGCACAGGAAACACATGAGGGAGTTACTGAAGATGGCATTGAA GACGCCTTTGAGGTTCTTGGTGAAATTCAGGAAATTGTGAAAACTGGGCTGTGGGTAGGTGACTGCTTCA TTTACACAAGTTCTGTGAACAGATTAAACTATTACGTGGGAGGAGAGATAGTCACCATCGCCCACCTGGA CAGGACAATGTATCTTCTGGGCTATATTCCAAAAGACAATAGACTTTATCTGGGGGATAAGGAATTGAAT ATTGTTAGCTACTCCCTGTTGGTTTCAGTCCTGGAATACCAGACTGCTGTCATGCGGAGGGATTTTAGCA TGGCGGATAAAGTCCTCCCTACCATCCCCAAAGAACAGAGGACCAGAGTTGCACACTTTTTGGAAAAACA GGGCTTCAAGCAGCAAGCACTTACAGTATCCACAGATCCTGAACATCGTTTTGAGCTTGCTCTTCAACTT GGAGAGTTGAAAATTGCTTACCAGTTAGCAGTGGAAGCAGAGTCGGAGCAGAAGTGGAAACAACTTGCTG AACTTGCCATTAGTAAATGTCAGTTCAGCCTAGCCCAGGAGTGTCTGCACCATGCTCAGGATTATGGTGG TCTGCTGCTCTTGGCCACTGCTTCTGGAAATGCTAGTATGGTGAACAAGCTGGCAGAAGGTGCGGAGAGA GACGGCAAAAATAACGTGGCATTCATGAGCTATTTTTTACAGGGCAAGCTTGATGCTTGCCTAGAGCTCT TGATCAGAACAGGAAGACTCCCAGAAGCTGCCTTCCTGGCCCGAACTTACTTACCCAGTCAGGTCTCAAG GGTGGTGAAACTGTGGAGGGAGAATCTCTCAAAAGTCAACCAGAAAGCAGCAGAATCCCTTGCTGATCCT ACAGAGTATGAAAACCTTTTCCCTGGATTAAAAGAAGCCTTTGTTGTTGAAGAATGGGTGAAGGAGACAC ATGCGGATTTGTGGCCAGCCAAGCAGTACCCTCTTGTCACGCCAAATGAAGAGAGAAATGTTATGGAAGA GGCAAAAGGCTTTCAGCCCTCAAGACCCACAGCCCAGCAGGAACCTGATGGAAAACCTGCTTCTAGCCCA GTTATCATGGCCTCCCAAACTACCCACAAAGAAGAAAAGAGTTTACTAGAACTAGAAGTTGATTTGGATA ACTTGGAATTAGAGGATATTGACACAACAGACATTAATCTGGATGAAGATATTCTGGATGATTAAAGTTT GCCATTTACTGCAGTAAGAGATTATTATGGCATTTTCAAATCAATCACCATCCTGACCAGAACTTTGGAA ATCCTTGATTTGCATATGTAAACAAGGGGGAACATAGCCACTTCATCTTCAGGAGTTGATGGGCAGCGTT TAAGTTACTATGTTCTCCTTGGTAACTGAAACAGAAACTTGCTTTTTTTCATATTGTTACTAGATCACCC GTCATAAAATCTTTTGAATTAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:3499585 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3499585 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10863 same embryo
	EMAGE:10861 same embryo
	EMAGE:10862 same embryo
	EMAGE:10859 same embryo
	EurExpress:euxassay_003332 same experiment
	MGI:4824004 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source