EMAGE mouse gene expression spatial database

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EMAGE:10862

Aktip thymoma viral proto-oncogene 1 interacting protein ( MGI:3693832)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003309. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003309_01	euxassay_003309_02	euxassay_003309_03	euxassay_003309_04

euxassay_003309_05	euxassay_003309_06	euxassay_003309_07	euxassay_003309_08	euxassay_003309_09

euxassay_003309_10	euxassay_003309_11	euxassay_003309_12	euxassay_003309_13	euxassay_003309_14

euxassay_003309_15	euxassay_003309_16	euxassay_003309_17	euxassay_003309_18	euxassay_003309_19

euxassay_003309_20	euxassay_003309_21	euxassay_003309_22	euxassay_003309_23	euxassay_003309_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10862_wholemount_strong.wlz

10862_wholemount_moderate.wlz

10862_wholemount_weak.wlz

10862_wholemount_possible.wlz

10862_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10862_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 17 18 19 20

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

moderate

regional

moderate expression: see section 24 weak expression: see section 07 08 23

facial vii ganglion

moderate

regional

moderate expression: see section 03 20 weak expression: see section 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 weak expression: see section 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 08 weak expression: see section 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 18 19 weak expression: see section 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 16

cervical ganglion

moderate

regional

moderate expression: see section 09 17

thoracic ganglion

moderate

regional

moderate expression: see section 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 14 15 16 18 19 20 weak expression: see section 17

bladder

weak

regional

weak expression: see section 15 16

kidney calyx

moderate

regional

moderate expression: see section 09 10 11 12 19 20 21 22

urethra of male

moderate

regional

moderate expression: see section 15 16 weak expression: see section 17

left lung

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12

right lung

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 22 23 24 weak expression: see section 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6221
Entity Detected:	Aktip, thymoma viral proto-oncogene 1 interacting protein ( MGI:3693832)
Sequence:	sense strand is shown >T6221 CGGTGGCGCTCGGCCCCCAGCGCTCCGCACCGAGGGCATCCAGGTTAAGGCAGAGGCAGGAAGATTGGGA GTTTGAGACCAGCCTGGGGTGTATCCTGAATTCCTGACTAACCTGGACCACATAGCAGTGTGAATGCTGA GGACAGAAGAGTTTGATGACACAGCGCTGAAGTTCTTTGTGTGGAGCACACGTAATGAACCCTTTGTGGA GCATGTCTGCAGGCTCTGTGCGCAAACGAGCTGAAGGTGAAGAGAAGACATTAGCAGGAGATGTGAAAAC CAGTCCTCCACGAAGTGCACCCAAAAAACAGCTACCTTCTATTCCCAAAAATGCTCTACCCATAGCGAAG CCTACATCTCCTGCCCCAGCAGCGCAGTCAACAAACGGCACCCATGCCTCTTATGGACCCTTCTACCTGG AATATTCGCTTCTTGCAGAATTTACTCTGGTAGTGAAGCAGAAGCTGCCAGGAGTCTACGTACAGCCATC TTACCGCTCTGCATTAGTGTGGTTTGGAGTAATATTCATACGGCACGGGCTGTATCAGGATGGCGTATTC AAGTTTACGGTGTATATTCCTGATAACTATCCTGATGGCGACTGTCCGCGCTTGCTGTTTGATATTCCCG TCTTTCACCCACTAGTTGATCCCACCTCGGGTGAGCTGGATGTGAAAAGAGCGTTCGCAAAGTGGAGGCG GAACCATAACCATATATGGCAGGTGTTAATGTATGCGAGGAGAGTCTTCTACAAGATCGACACGACGAGC CCCCTGAACCCAGAGGCCGCAGTGCTGTATGAAAAAGACATTCAACTTTTTAAAAGTAAAGTGGTCGACA GTGTTAAAGTGTGTACTGCCCGTCTTTTTGACCAGCCCAAGATAGAAGACCCCTACGCCATTAGCTTTTC TCCGTGGAACCCTTCTGTACATGATGAGGCCAGAGAAAAGATGCTGACTCAGAAGAAGCCTGATGAACAG CACAATAAAAGTGTTCATGTTGCTGGCCTGTCATGGGTAAAGCCTGGCTCAGTACAACCTTTCAGTAAAG AAGAGAAAACAGTAGCAACCTAAGAGATGGTGGATCTGGTGCACCGTGCACTTTCCTGCTGGACTCTGGC CTAGTTAAAGCTGACTAATGGCAAAGGACTGCCTGAAGAGTAAACTTCGTGAACCGTGACTGGCTATCAG TGTTGTGTATGCGTAGGTTCTAACAGCAGGTTATGGAGACCTCTCATTAAGTAGAGTTACTTCTGTCGGA AGTGTCAGGGTGGATCTAGTTCAGTACTCCAAATTATTGGGGACCTTGAGGCTTAAGTATTTTTCTGAAT ATGTTAGAGGTAAGTTGTGTTTACTTAAACTAACAGAACAGAGAGACTGTAATGAGTACAATGAGTACCA TGTAATAGTTTGGAGTTGGTAGTTTCAGCTGTACATATGTTAGGCCATGAAGAAGGGTGTAGAGGACAGA CTCCGAGTGCAGCACTTTCAAGTAGAAGCCTGGCTTCCCAGCTTCAGCCTGCTGACTTGCTGTAAACATC TATCTCAGAAGCATTAGGAACGTAGGCAGAACCAGGATGCTTCATCATAGGGTTTTTTTTTTTTATTTTT TTTTTTAAGGCAAATTACTGTATTTTTATGGCAGGAAGAAGAAAAAAGTGTTCAAACGGTTTCTGATGAA GTCAGGTATTTAAATGGTGAATGACTGATGTGTTAGTGGAGATGAAATAAACCAATAAATGATTGATTGT CATTTATGCAGGAAAGTAATGCTCCTTTTCAATATAACTAAACAGAGACTAATTTATAAGTGCTTTATTG AAAAATACACATATTTTCATATAAAATTACAGTAGCGGTACTAAGGTTTTCTAAGTACTCTGCACAGCAC TACTCTGTGGTGGGGGGGGAGTGCCAGGCTGTAGGTCCTCAGCTGGGTGCTGTAGAGCAGCATGAAGTTA CCTAGTAACTTGGTTTATCATGATCCAATTTACAATTGAATTTTCTCCTTAGGATTATTAATCCAACAAT AATGATTAATAAAGATATGCGTAGATAATCAATAGCTGTTAAATAATTTTCTAATTTGTATAAATGGTAC TATGTACTAATAAAAACCTCAGTTCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:3498984 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3498984 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10863 same embryo
	EMAGE:10861 same embryo
	EMAGE:10860 same embryo
	EMAGE:10859 same embryo
	EurExpress:euxassay_003309 same experiment
	MGI:4823075 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source