EMAGE mouse gene expression spatial database

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EMAGE:10869

Ext1 exostoses (multiple) 1 ( MGI:894663)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003308. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003308_01	euxassay_003308_02	euxassay_003308_03	euxassay_003308_04

euxassay_003308_05	euxassay_003308_06	euxassay_003308_07	euxassay_003308_08	euxassay_003308_09

euxassay_003308_10	euxassay_003308_11	euxassay_003308_12	euxassay_003308_13	euxassay_003308_14

euxassay_003308_15	euxassay_003308_16	euxassay_003308_17	euxassay_003308_18	euxassay_003308_19

euxassay_003308_20	euxassay_003308_21	euxassay_003308_22	euxassay_003308_23	euxassay_003308_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10869_wholemount_strong.wlz

10869_wholemount_moderate.wlz

10869_wholemount_weak.wlz

10869_wholemount_possible.wlz

10869_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10869_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand mesenchyme

moderate

regional

moderate expression: see section 02

hand skin

moderate

regional

moderate expression: see section 01 02 22 23 weak expression: see section 24

foot mesenchyme

moderate

regional

moderate expression: see section 02 04 07 weak expression: see section 24

foot skin

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 22 23 weak expression: see section 24

submandibular gland primordium

moderate

regional

moderate expression: see section 05 06 07 08 17 18 19 weak expression: see section 16

hypothalamus ventricular layer

weak

regional

weak expression: see section 10 11 12

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 09 10 11 12

telencephalon ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 13 14 15 16 17 18

rest of cerebellum marginal layer

weak

regional

weak expression: see section 13

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 16 17 18 19 20 weak expression: see section 12 14 15

pons ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 16 17 weak expression: see section 13 15

midbrain ventricular layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 weak expression: see section 13 15

ventral grey horn

moderate

regional

moderate expression: see section 14 weak expression: see section 09 10 11 12 13 15 16

extrinsic ocular muscle

moderate

regional

moderate expression: see section 02 03 04 05 06 19 20 21 weak expression: see section 01 22 23 24

viscerocranium

weak

regional

Expression in the turbinate bone.

heart valve

moderate

regional

moderate expression: see section 09 12 weak expression: see section 10 11 13

tongue muscle

weak

regional

weak expression: see section 09 10 11 12 13 14 15

stomach

weak

regional

weak expression: see section 03 04 05 06 07 08 09

midgut

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 14 15 16

lower jaw molar

moderate

regional

moderate expression: see section 18 19 weak expression: see section 05 06

upper jaw incisor

moderate

regional

moderate expression: see section 10 11 15 16

upper jaw molar

moderate

regional

moderate expression: see section 18 19 weak expression: see section 05 06 07

genital tubercle of male

weak

regional

weak expression: see section 11 12 13 14 15

urethra of male

weak

regional

weak expression: see section 13 14

lung

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

trachea

moderate

regional

moderate expression: see section 13 weak expression: see section 12

sternum

weak

regional

weak expression: see section 10 11 12 13 14 15

pelvic girdle skeleton

moderate

regional

moderate expression: see section 09 18 weak expression: see section 19

tail mesenchyme

weak

regional

weak expression: see section 11 15 16

axial skeleton tail region

weak

regional

weak expression: see section 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6240
Entity Detected:	Ext1, exostoses (multiple) 1 ( MGI:894663)
Sequence:	sense strand is shown >T6240 CCCACGCGTCCGCCACGCGTCCGGATCAGTCCACGGCTTGGGGAAAGGCATCCAGAGAAGGTGGGAGCGG AGAGTTTGAAGTCTTTACAGGCGGGAAGATGGCGGACTGGAGCTGAAAGTGTTGATTGGGAAACTTGGGT GATTCTTGTGTTTATTTACAATCCTCTTGACCCAGGCAGGACACATGCAGGCCAAAAAACGCTATTTCAT CCTGCTCTCAGCTGGCTCTTGTCTCGCCCTTTTGTTTTATTTTGGAGGCGTGCAGTTTAGGGCATCGAGG AGCCACAGCCGGAGAGAAGAGCACAGTGGTCGGAATGGCTTGCACCAGCCCAGTCCGGATCATTTCTGGC CCCGCTTCCCGGACGCTCTGCGCCCTTTCTTTCCTTGGGATCAATTGGAAAACGAGGATTCCAGCGTGCA CATTTCCCCCCGGCAGAAGCGAGACGCCAACTCGAGCATCTACAAAGGCAAGAAGTGCCGCATGGAGTCC TGCTTCGATTTCACCCTTTGCAAGAAAAACGGCTTCAAAGTCTACGTGTACCCGCAGCAGAAAGGGGAGA AAATCGCCGAAAGTTACCAAAACATTCTAGCGGCCATCGAGGGCTCCAGGTTCTACACCTCGGACCCCAG CCAGGCGTGCCTCTTTGTCTTGAGTCTGGATACTTTAGACAGAGACCAGTTATCACCTCAGTATGTGCAC AATTTGAGATCCAAAGTGCAGAGTCTCCACTTGTGGAACAATGGTAGGAATCATTTAATTTTTAATTTAT ATTCTGGCACTTGGCCTGACTACACTGAGGACGTGGGGTTTGACATCGGCCAGGCGATGCTGGCCAAAGC CAGCATCAGTACTGAAAACTTCCGACCAAACTTTGATGTTTCTATTCCCCTCTTTTCTAAGGATCATCCC AGGACAGGAGGGGAGAGGGGGTTTTTGAAATTTAACACCATCCCTCCTCTCAGGAAGTACATGCTGGTAT TCAAGGGGAAGCGGTACCTGACAGGGATAGGGTCAGACACCAGGAATGCCTTATATCACGTCCATAACGG GGAGGACGTCTTGCTCCTCACCACCTGCAAGCATGGCAAAGACTGGCAAAAGCACAAGGATTCTCGCTGT GACAGAGACAACACCGAGTATGAGAAATATGATTATCGGGAAATGCTGCACAATGCCACTTTCTGTCTGG TTCCTCGTGGTCGCAGGCTTGGGTCCTTCAGATTCCTGGAGGCTTTGCAGGCTGCCTGTGTCCCTGTAAT GCTCAGCAACGGATGGGAGTTGCCATTCTCCGAAGTGATTAATTGGAACCAAGCTGCCGTCATAGGCGAT GAGAGATTGCTATTACAGATTCCTTCTACAATCAGGTCTATTCATCAGGATAAAATCCTAGCACTTAGAC AGCAGACACAGTTCTTGTGGGAGGCTTATTTTTCTTCAGTTGAGAAGATTGTATTAACTACACTAGAGAT TATTCAGGACAGAATATTCAAGCACATATCACGTAACAGTTTAATATGGAACAAACATCCTGGAGGATTG TTCGTCCTACCGCAGTATTCATCTTACCTGGGAGATTTCCCTTACTACTATGCTAATTTAGGTTTAAAGC CCCCCTCCAAATTCACTGCAGTCATCCATGCTGTGACTCCCCTGGTCTCTCAGTCCCAGCCAGTGTTGAA GCTTCTTGTGGCTGCAGCCAAATCCCAGTACTGTGCGCAGATCATAGTTCTGTGGAATTGTGACAAGCCT CTACCAGCCAAACATCGCTGGCCTGCCACTGCCGTGCCTGTCATCGTCATTGAAGGAGAAAGCAAGGTTA TGAGCAGCCGGTTTCTGCCCTATGACAACATCATCACTGATGCTGTGCTCAGCCTGGATGAGGACACTGT GCTTTCAACTACGGAAGTGGATTTTGCCTTCACCGTATGGCAGAGCTTCCCAGAGAGGATTGTGGGATAT CCTGCTCGCAGTCATTTCTGGGATAACTCTAAGGAGCGGTGGGGATATACATCCAAGTGGACGAATGACT ACTCCATGGTGTTGACAGGAGCTGCTATCTACCACAAATATTATCACTACCTGTATTCCCATTACCTGCC AGCCAGCCTGAAGAACATGGTAGACCAACTGGCCAACTGTGAGGACATTCTCATGAATTTCCTGGTGTCT GCTGTGACAAAATTGCCTCCAATCAAAGTGACCCAGAAGAAACAGTATAAGGAGACAATGATGGGACAGA CTTCCCGAGCATCCCGCTGGGCCGACCCTGACCACTTTGCCCAGCGACAGAGCTGCATGAATACATTTGC CAGCTGGTTTGGCTACATGCCGCTGATCCATTCTCAGATGAGGCTGGACCCGGTCCTCTTTAAAGACCAA GTCTCAATTCTGAGGAAGAAATACAGAGACATTGAACGACTTTGAGGAAGCCCACCGAGTGGGGGAGGGG AAGCAAGATGGGCGTCCAGCTGCTCTCTCCTCCTTCCCAATGCAGATCCGCTCACGCCCAGCAGTGGAGC CAGACTGTGCCAAGTATCAAAAAATCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3499743 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3499743 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10871 same embryo
	EMAGE:10873 same embryo
	EMAGE:10874 same embryo
	EMAGE:10870 same embryo
	EMAGE:10872 same embryo
	EurExpress:euxassay_003308 same experiment
	MGI:4824651 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source